[논문]Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524

Yang, Jung-Mo; Moon, Gi-Seong

doi:10.5851/kosfa.2020.e98

Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524 원문보기

Food science of animal resources, v.41 no.1, 2021년, pp.164 - 171

Yang, Jung-Mo (Major of Biotechnology, Korea National University of Transportation) , Moon, Gi-Seong (Major of Biotechnology, Korea National University of Transportation)

Abstract ▼ AI-Helper

Listeria monocytogenes is a representative foodborne pathogen and causes listeriosis. Enterococcus faecium CJNU 2524 was confirmed to produce a bacteriocin with anti-listerial activity. To establish optimal culture conditions for the production of the bacteriocin from E. faecium CJNU 2524, different media (MRS and BHI broth) and temperatures (25℃, 30℃, and 37℃) were investigated. The results showed that the optimal culture conditions were MRS broth and 25℃ or 30℃ temperatures. The crude bacteriocin was stable in a broad range of pH conditions (2.0-10.0), temperatures (60℃-100℃), and organic solvents (methanol, ethanol, acetone, acetonitrile, and chloroform). The bacteriocin activity was abolished when treated with protease but not α-amylase or lipase, indicating the proteinaceous nature of the bacteriocin. Finally, the bacteriocin showed a bactericidal mode of action against L. monocytogenes. Therefore, it can be a biopreservative candidate for controlling L. monocytogenes in dairy and meat products.

주제어

표/그림 (3)

그림 Fig. 1. Optimal culture conditions for production of bacteriocin from Enterococcus faecium CJNU 2524. Culture medium panel: optical densities in BHI broth (A) and MRS broth (B), bacteriocin activities in BHI broth (C) and MRS broth (D); Culture temperature panel: optical densities at 37℃ (A), 30℃ (B), and 25℃ (C), bacteriocin activities at 25℃ (D), 30℃ (E), and 37℃ (F). The asterisk (*) indicates significance of mean values between two items (*** p<0.001) and the mean values with different letters (a-c) at times are significantly different (p<0.05).
표 Table 1. Stability of the crude bacteriocin of Enterococcus faecium CJNU 2524
그림 Fig. 2. Stability of bacteriocin from Enterococcus faecium CJNU 2524 after treatment of enzymes (A) and mode of action for the bacteriocin against Listeria monocytogenes (B). 1: control; 2: protease; 3: lipase; 4: α-amylase. The asterisk (*) indicates significance of mean values when compared with control (*** p<0.001).

AI 본문요약
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제안 방법

The crude bacteriocin was added into the inoculum with 10 and 100 AU/mL as final concentrations and the viable L. monocytogenes KCTC 3569 cell counts (CFU/mL) were measured at times (0, 1, 2, 3, 4, 5, and 6 h) to determine the mode of action, i.e., bacteriostatic or bactericidal.

데이터처리

25 (SPSS Inc., Chicago, IL, USA) with t-test or one-way ANOVA where post hoc analysis was done by Duncan’s multiple range test (p<0.05).

참고문헌 (29)

Aymerich T Artigas MG Garriga M Monfort JM Hugas M 2000 Effect of sausage ingredients and additives on the production of enterocin A and B by Enterococcus faecium CTC492. Optimization of in vitro production and anti-listerial effect in dry fermented sausages J Appl Microbiol 88 686 694 10.1046/j.1365-2672.2000.01012.x 10792528

상세보기
Chan YC Wiedmann M 2008 Physiology and genetics of Listeria monocytogenes survival and growth at cold temperatures Crit Rev Food Sci Nutr 49 237 253 10.1080/10408390701856272 19093268

상세보기
Chung DM Kim KE Jeong SY Park CS Ahn KH Kim DH Kang DO Chun HK Yoon BD Koh HB Kim HJ Choi NS 2011 Rapid concentration of some bacteriocin-like compounds using an organic solvent Food Sci Biotechnol 20 1457 1459 10.1007/s10068-011-0201-8

원문보기 상세보기
Cui Y Zhang C Wang Y Shi J Zhang L Ding Z Qu X Cui H 2012 Class IIa bacteriocins: Diversity and new developments Int J Mol Sci. 13 16668 16707 10.3390/ijms131216668 23222636

상세보기
Daeschel MA 1992 Procedures to detect antimicrobial activities of microorganisms In Food biopreservatives of microbial origin Ray B Daeschel M (ed) CRC Press Boca Raton, FL, USA 57 80
Farber JM Peterkin PI 1991 Listeria monocytogenes , a food-borne pathogen Microbiol Rev 55 476 511 10.1128/MR.55.3.476-511.1991 1943998

상세보기
Gabrielsen C Brede DA Nes IF Diep DB 2014 Circular bacteriocins: biosynthesis and mode of action Appl Environ Microbiol 80 6854 6862 10.1128/AEM.02284-14 25172850

상세보기
Henderson LO Erazo Flores BJ Skeens J Kent D Murphy SI Wiedmann M Guariglia-Oropeza V 2020 Nevertheless, she resisted - Role of the environment on Listeria monocytogenes sensitivity to nisin treatment in a laboratory cheese model Front Microbiol 11 635 10.3389/fmicb.2020.00635 32328054

상세보기
Heredia N García S 2018 Animals as sources of food-borne pathogens: A review Anim Nutr 4 250 255 10.1016/j.aninu.2018.04.006 30175252

상세보기
Ibarra-Sánchez LA El-Haddad N Mahmoud D Miller MJ Karam L 2020 Invited review: Advances in nisin use for preservation of dairy products J Dairy Sci 103 2041 2052 10.3168/jds.2019-17498 31928749

상세보기
Indrawattana N Nibaddhasobon T Sookrung N Chongsa-Nguan M Tungtrongchitr A Makino S Tungyong W Chaicumpa W 2011 Prevalence of Listeria monocytogenes in raw meats marketed in Bangkok and characterization of the isolates by phenotypic and molecular methods J Health Popul Nutr 29 26 38 10.3329/jhpn.v29i1.7565 21528788

상세보기
Kale SB Kurkure NV Doijad SP Poharkar KV Garg S Rawool DB Barbuddhe SB 2017 Variations in stress tolerance abilities of diverse Listeria monocytogenes isolates Int J Curr Microbiol Appl Sci 6 2246 2258 10.20546/ijcmas.2017.605.250
Kang JH Lee MS 2005 Characterization of a bacteriocin produced by Enterococcus faecium GM-1 isolated from an infant J Appl Microbiol 98 1169 1176 10.1111/j.1365-2672.2005.02556.x 15836487

상세보기
Khelissa S Chihib NE Gharsallaoui A 2020 Conditions of nisin production by Lactococcus lactis subsp. lactis and its main uses as a food preservative Arch Microbiol (in press) 10.1007/s00203-020-02054-z 33001222

상세보기
Komora N Maciel C Pinto CA Ferreira V Brandão TRS Saraiva JMA Castro SM Teixeira P 2020 Non-thermal approach to Listeria monocytogenes inactivation in milk: The combined effect of high pressure, pediocin PA-1 and bacteriophage P100 Food Microbiol 86 103315 10.1016/j.fm.2019.103315 31703881

상세보기
Kubašová I Diep DB Ovchinnikov KV Lauková A Strompfová V 2020 Bacteriocin production and distribution of bacteriocin-encoding genes in enterococci from dogs Int J Antimicrob Agents 55 105859 10.1016/j.ijantimicag.2019.11.016 31794868

상세보기
Lecuit M 2007 Human listeriosis and animal models Microbes Infect 9 1216 1225 10.1016/j.micinf.2007.05.009 17720601

상세보기
Lee J Seo Y Ha J Kim S Choi Y Oh H Lee Y Kim Y Kang J Park E Yoon Y 2020 Influence of milk microbiota on Listeria monocytogenes survival during cheese ripening Food Sci Nutr 8 5071 5076 10.1002/fsn3.1806 32994967

상세보기
Leong D Alvarez-Ordóñez A Jordan K 2014 Monitoring occurrence and persistence of Listeria monocytogenes in foods and food processing environments in the Republic of Ireland Front Microbiol 5 436 10.3389/fmicb.2014.00436 25191314

상세보기
Lim JY Lee CL Kim GH Bang YJ Rhim JW Yoon KS 2020 Using lactic acid bacteria and packaging with grapefruit seed extract for controlling Listeria monocytogenes growth in fresh soft cheese J Dairy Sci 103 8761 8770 10.3168/jds.2020-18349 32713695

상세보기
Maertens de Noordhout C Devleesschauwer B Angulo FJ Verbeke G Haagsma J Kirk M Havelaar A Speybroeck N 2014 The global burden of listeriosis: A systematic review and meta-analysis Lancet Infect Dis 14 1073 1082 10.1016/S1473-3099(14)70870-9 25241232

상세보기
Nyarko EB Donnelly CW 2015 Listeria monocytogenes : Strain heterogeneity, methods, and challenges of subtyping J Food Sci 80 M2868 M2878 10.1111/1750-3841.13133 26588067

상세보기
O’Connor PM Kuniyoshi TM Oliveira RP Hill C Ross RP Cotter PD 2020 Antimicrobials for food and feed; a bacteriocin perspective Curr Opin Biotechnol 61 160 167 10.1016/j.copbio.2019.12.023 31968296

상세보기
Pandey P Hansmann UHE Wang F 2020 Altering the solubility of the antibiotic candidate nisin: A computational study ACS Omega 5 24854 24863 10.1021/acsomega.0c03594 33015504

상세보기
Qiao X Du R Wang Y Han Y Zhou Z 2020 Purification, characterization and mode of action of enterocin, a novel bacteriocin produced by Enterococcus faecium TJUQ1 Int J Biol Macromol 144 151 159 10.1016/j.ijbiomac.2019.12.090 31846663

상세보기
Todorov SD Kang HJ Ivanova IV Holzapfel WH 2020 Bacteriocins from LAB and other alternative approaches for the control of Clostridium and Clostridiodes related gastrointestinal colitis Front Bioeng Biotechnol 8 581778 10.3389/fbioe.2020.581778 33042979

상세보기
Vijayakumar PP Muriana PM 2017 Inhibition of Listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based Foods 6 1 13 10.3390/foods6030022 28335414

상세보기
Wieczorek K Dmowska K Osek J 2012 Prevalence, characterization, and antimicrobial resistance of Listeria monocytogenes isolates from bovine hides and carcasses Appl Environ Microbiol 78 2043 2045 10.1128/AEM.07156-11 22247138

상세보기
Yu HH Song MW Song YJ Lee NK Paik HD 2019 Antibacterial effect of a mixed natural preservative against Listeria monocytogenes on lettuce and raw pork loin J Food Prot 82 2001 2006 10.4315/0362-028X.JFP-19-026 31644336

상세보기

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