[논문]High Prevalence of Listeria monocytogenes in Smoked Duck: Antibiotic and Heat Resistance, Virulence, and Genetics of the Isolates

Park, Eunyoung; Ha, Jimyeong; Oh, Hyemin; Kim, Sejeong; Choi, Yukyung; Lee, Yewon; Kim, Yujin; Seo, Yeongeun; Kang, Joohyun; Yoon, Yohan

doi:10.5851/kosfa.2021.e2

[국내논문] High Prevalence of Listeria monocytogenes in Smoked Duck: Antibiotic and Heat Resistance, Virulence, and Genetics of the Isolates 원문보기

Food science of animal resources, v.41 no.2, 2021년, pp.324 - 334

Park, Eunyoung (Department of Food and Nutrition, Sookmyung Women's University) , Ha, Jimyeong (Risk Analysis Research Center, Sookmyung Women's University) , Oh, Hyemin (Department of Food and Nutrition, Sookmyung Women's University) , Kim, Sejeong (Risk Analysis Research Center, Sookmyung Women's University) , Choi, Yukyung (Risk Analysis Research Center, Sookmyung Women's University) , Lee, Yewon (Department of Food and Nutrition, Sookmyung Women's University) , Kim, Yujin (Department of Food and Nutrition, Sookmyung Women's University) , Seo, Yeongeun (Department of Food and Nutrition, Sookmyung Women's University) , Kang, Joohyun (Department of Food and Nutrition, Sookmyung Women's University) , Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University)

Abstract ▼ AI-Helper

This study aimed at determining the genetic and virulence characteristics of the Listeria monocytogenes from smoked ducks. L. monocytogenes was isolated by plating, and the isolated colonies were identified by PCR. All the obtained seven L. monocytogenes isolates possessed the virulence genes (inlA, inlB, plcB, and hlyA) and a 385 bp actA amplicon. The L. monocytogenes isolates (SMFM2018 SD 1-1, SMFM 2018 SD 4-1, SMFM 2018 SD 4-2, SMFM 2018 SD 5-2, SMFM 2018 SD 5-3, SMFM 2018 SD 6-2, and SMFM 2018 SD 7-1) were inoculated in tryptic soy broth (TSB) containing 0.6% yeast extract at 60℃, followed by cell counting on tryptic soy agar (TSA) containing 0.6% yeast extract at 0, 2, 5, 8, and 10 min. We identified five heat resistant isolates compared to the standard strain (L. monocytogenes ATCC13932), among which three exhibited the serotype 1/2b and D-values of 5.41, 6.48, and 6.71, respectively at 60℃. The optical densities of the cultures were regulated to a 0.5 McFarland standard to assess resistance against nine antibiotics after an incubation at 30℃ for 24 h. All isolates were penicillin G resistant, possessing the virulence genes (inlA, inlB, plcB, and hlyA) and the 385-bp actA amplicon, moreover, three isolates showed clindamycin resistance. In conclusion, this study allowed us to characterize L. monocytogenes isolates from smoked ducks, exhibiting clindamycin and penicillin G resistance, along with the 385-bp actA amplicon, representing higher invasion efficiency than the 268-bp actA, and the higher heat resistance serotype 1/2b.

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표/그림 (8)

표 Table 1. Primers used for the identification of the Listeria monocytogenes isolates
표 Table 2. Primers used for the PCR amplification of the virulence genes from the Listeria monocytogenes isolates
그림 Fig. 1. PCR for seven pathogenic Listeria monocytogenes isolates from smoked ducks using primers targeting hlyA genes.
그림 Fig. 2. PCR amplification of the actA sequence of Listeria monocytogenes isolates from smoked ducks. (A) annealing at 55℃ for actA amplification, (B) annealing at 57℃ for actA amplification, and (C) annealing at 60℃ for actA amplification.
표 Table 3. Serotypes and information of the Listeria monocytogenes isolates from smoked ducks
표 Table 4. Antibiotic sensitivity of Listeria monocytogenes isolates from smoked ducks (n=7)
그림 Fig. 3. Survival of seven Listeria monocytogenes isolates from smoked ducks after exposure to heat treatment at 60℃ for 10 min. * Statically significant, compared to the standard strain by pairwise t-test (p<0.05).
표 Table 5. D-values (mean±SD) of Listeria monocytogenes from smoked duck in TSBYE at 60℃

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성능/효과

In conclusion, this study showed a high smoked duck product-derived L. monocytogenes prevalence in Korea.

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