4,4'-Diaponeurosporene from Lactobacillus plantarum subsp. plantarum KCCP11226: Low Temperature Stress-Induced Production Enhancement and In Vitro Antioxidant Activity원문보기
Kim, Mibang
(Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University)
,
Jung, Dong-Hyun
(Bacteria Research Team, Nakdonggang National Institute of Biological Resources)
,
Seo, Dong-Ho
(Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University)
,
Park, Young-Seo
(Department of Food Science and Biotechnology, Gachon University)
,
Seo, Myung-Ji
(Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University)
Carotenoids, which have biologically beneficial effects and occur naturally in microorganisms and plants, are pigments widely applied in the food, cosmetics and pharmaceutical industries. The compound 4,4'-diaponeurosporene is a C30 carotenoid produced by some Lactobacillus species, and Lactobacillu...
Carotenoids, which have biologically beneficial effects and occur naturally in microorganisms and plants, are pigments widely applied in the food, cosmetics and pharmaceutical industries. The compound 4,4'-diaponeurosporene is a C30 carotenoid produced by some Lactobacillus species, and Lactobacillus plantarum is the main species producing it. In this study, the antioxidant activity of 4,4'-diaponeurosporene extracted from L. plantarum subsp. plantarum KCCP11226 was examined. Maximum carotenoid content (0.74 ± 0.2 at A470) was obtained at a relatively low temperature (20℃). The DPPH radical scavenging ability of 4,4'-diaponeurosporene (1 mM) was approximately 1.7-fold higher than that of butylated hydroxytoluene (BHT), a well-known antioxidant food additive. In addition, the ABTS radical scavenging ability was shown to be 2.3- to 7.5-fold higher than that of BHT at the range of concentration from 0.25 mM to 1 mM. The FRAP analysis confirmed that 4,4'-diaponeurosporene (0.25 mM) was able to reduce Fe3+ by 8.0-fold higher than that of BHT. Meanwhile, 4,4'-diaponeurosporene has been confirmed to be highly resistant to various external stresses (acid/bile, high temperature, and lysozyme conditions). In conclusion, L. plantarum subsp. plantarum KCCP11226, which produces 4,4'-diaponeurosporene as a functional antioxidant, may be a potentially useful strain for the development of functional probiotic industries.
Carotenoids, which have biologically beneficial effects and occur naturally in microorganisms and plants, are pigments widely applied in the food, cosmetics and pharmaceutical industries. The compound 4,4'-diaponeurosporene is a C30 carotenoid produced by some Lactobacillus species, and Lactobacillus plantarum is the main species producing it. In this study, the antioxidant activity of 4,4'-diaponeurosporene extracted from L. plantarum subsp. plantarum KCCP11226 was examined. Maximum carotenoid content (0.74 ± 0.2 at A470) was obtained at a relatively low temperature (20℃). The DPPH radical scavenging ability of 4,4'-diaponeurosporene (1 mM) was approximately 1.7-fold higher than that of butylated hydroxytoluene (BHT), a well-known antioxidant food additive. In addition, the ABTS radical scavenging ability was shown to be 2.3- to 7.5-fold higher than that of BHT at the range of concentration from 0.25 mM to 1 mM. The FRAP analysis confirmed that 4,4'-diaponeurosporene (0.25 mM) was able to reduce Fe3+ by 8.0-fold higher than that of BHT. Meanwhile, 4,4'-diaponeurosporene has been confirmed to be highly resistant to various external stresses (acid/bile, high temperature, and lysozyme conditions). In conclusion, L. plantarum subsp. plantarum KCCP11226, which produces 4,4'-diaponeurosporene as a functional antioxidant, may be a potentially useful strain for the development of functional probiotic industries.
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문제 정의
In addition, the availability of strain KCCP11226 for probiotic use was investigated through in vitro experiments on safety and functionalities such as antibiotic resistance, cell surface hydrophobicity, and stress tolerance, including acid/bile, high temperature, and lysozyme. To the best of our knowledge, this study is the first report on the antioxidant activity of 4, 4′-diaponeurosporene produced by Lactobacillus.
대상 데이터
plantarum KCCP11226 was previously isolated from a Korean fermented food [8]. L.rhamnosus GG (KCTC 5033), which was used as reference strain in this study, was purchased from the Korean Collection for Type Cultures (KCTC, Korea). De Man Rogosa and Sharpe (MRS) broth was purchased from MBcell (Korea) and oxgall was obtained from Difco Laboratories (USA).
plantarum subsp. plantarum KCCP11226 was tested. As a result, it was confirmed to have resistance to erythromycin, gentamicin, ciprofloxacin, lincomycin, tetracycline, and streptomycin, but not against ampicillin and novobiocin (Table S1).
데이터처리
The results from three independent tests are represented as means ± SD. Significant means were expressed after analysis of variance (ANOVA) with Tukey-Kramer multiple comparison tests (*p < 0.01).
The significance of differences in means was evaluated using analysis of variance (ANOVA) with Tukey-Kramer multiple comparison tests, unless mentioned otherwise. The FRAP data was analyzed using an unpaired t-test with Welch’s correction. Differences with p values of less than 0.
The significance of differences in means was evaluated using analysis of variance (ANOVA) with Tukey-Kramer multiple comparison tests, unless mentioned otherwise. The FRAP data was analyzed using an unpaired t-test with Welch’s correction.
성능/효과
plantarum KCCP11226 was tested. As a result, it was confirmed to have resistance to erythromycin, gentamicin, ciprofloxacin, lincomycin, tetracycline, and streptomycin, but not against ampicillin and novobiocin (Table S1). The ability to adhere to the intestinal mucosa is a prerequisite for bacterial colonization and is one of the most important properties for ideal probiotic strains.
4A). At 0.25, 0.5, and 1 mM, 4, 4′- diaponeurosporene had 65.3 ± 2.5%, 87.4 ± 0.7%, and 92.0 ± 4.9% DPPH radical scavenging activity, respectively, whereas BHT showed 23.1 ± 0.4%, 35.7 ± 1.3%, and 53.2 ± 1.0%, respectively. In previous studies, fucoxanthin showed 70% DPPH radical scavenging activity at a concentration of 300 mM [11].
후속연구
However, most of these studies have been limited to yeast producing C40 carotenoids [26], while carotenogenesis studies targeting lactic acid bacteria have not been actively conducted. Therefore, further studies are needed on the strategies for improving the production yield of microbial carotenoid, in particular carotenoid-biosynthesizing lactic acid bacteria as alternative sources of natural C30 carotenoids.
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