[논문]Histology and lectin histochemistry in the vomeronasal organ of Korean native cattle, Bos taurus coreanae

Jang, Sungwoong; Kim, Bohye; Kim, Joong-Sun; Moon, Changjong

doi:10.12750/jarb.36.4.270

Histology and lectin histochemistry in the vomeronasal organ of Korean native cattle, Bos taurus coreanae 원문보기

Journal of animal reproduction and biotechnology = 한국동물생명공학회지, v.36 no.4, 2021년, pp.270 - 284

Jang, Sungwoong (Department of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR program, Chonnam National University) , Kim, Bohye (Department of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR program, Chonnam National University) , Kim, Joong-Sun (Department of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR program, Chonnam National University) , Moon, Changjong (Department of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR program, Chonnam National University)

Abstract ▼ AI-Helper

The vomeronasal organ (VNO) is critical for reproduction and social behavior in ruminants, including cattle. The present study examined the structure of the VNO and its epithelial cells in neonatal and adult Korean native cattle (Hanwoo), Bos taurus coreanae, using immunohistochemistry and lectin histochemistry. Histologically, the VNO comprised two types of epithelia: medial vomeronasal sensory (VSE) and lateral vomeronasal non-sensory epithelia (VNSE). Numerous blood vessels and nerve bundles were observed within the vomeronasal cartilage encased lamina propria. Immunohistochemistry revealed high expression level of protein gene product9.5 and moderate expression level of olfactory marker protein in the neuroreceptor cells of the VSE and occasionally in some ciliated cells of the VNSE in both neonates and adults. The properties of the glycoconjugates in the VNO were investigated using 21 lectins, most of which were expressed at varied intensities in the VSE and VNSE, as well as in the lamina propria. Several lectins exhibited variations in their intensities and localization between neonatal and adult VNOs. This study is the first descriptive lectin histochemical examination of the VNO of Korean native cattle with a focus on lectin histochemistry, confirming that the VNO of Korean native cattle is differentiated during postnatal development.

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표/그림 (11)

표 Table 1. Summary of the lectins used, including source, sugar specificity, concentration, and inhibitor
그림 Fig. 1. Histological examination of VNOs from Korean native cattle. (A, B) Low-magnification views of the VNO in neonates (A) and adults (B). (C, D) High-magnification views of the VSE (C) and VNSE (D) in neonates. (E, F) High-magnification views of the VSE (E) and VNSE (F) in adults. bv, blood vessels; VBC, vomeronasal basal cell; VNc, vomeronasal cartilage; VNg, vomeronasal glands; VNn, vomeronasal nerves; VNO, vomeronasal organ; VSC, vomeronasal supporting cell; VSE, vomeronasal sensory epithelium; VNSE, vomeronasal non-sensory epithelium; VRC, vomeronasal receptor cell. Hematoxylin and eosin staining. Scale bars = 500 μm (A, B), 50 μm (C-F).
그림 Fig. 2. Histological features of vomeronasal glands in Korean native cattle. (A, B) Vomeronasal glands stained with periodic acid-Schiff (PAS) in neonates (A) and adults (B). (C, D) Vomeronasal glands stained with Alcian blue (pH 2.5) in neonates (C) and adults (D). Scale bars = 25 μm.
표 Table 2. Expression patterns of PGP9.5 and OMP in the vomeronasal organ (VNO) of Korean native cattle
그림 Fig. 3. Immunohistochemical staining of VNOs from Korean native cattle. (A–D) PGP9.5 is intensely labeled in receptor cells in the VSE of both neonates (A) and adults (C), and some nerve fibers and ciliated cells also showed reactions in the VNSE of neonates (B) and adults (D). PGP9.5 is also expressed in the vomeronasal nerve bundle (A, C; asterisks). (E–H) OMP is weakly labeled in the VSE of neonates (C) and adults (G), and some ciliated cells showed reactions in VNSE of neonates (D) and adults (H). Counterstained with hematoxylin. OMP, olfactory marker protein; PGP9.5, protein gene product9.5; VNSE, vomeronasal non-sensory epithelium; VSE, vomeronasal sensory epithelium. Scale bars = 50 μm.
표 Table 3. Lectin-binding pattern in the vomeronasal sensory epithelium (VSE) of Korean native cattle
그림 Fig. 4. Lectin histochemistry with N-acetylglucosamine and mannose-binding lectins in the VSE of Korean native cattle. (A-F) The reactivities of N-acetylglucosamine-binding lectins, including s-WGA (A, B), DSL (C, D), and STL (E, F) in both neonatal (A, C, E) and adult VSE (B, D, F). (G-J) The reactivities of mannose-binding lectins, including LCA (G, H) and PSA (I, J) in both neonatal (G, I) and adult VSE (I, J). Counterstained with hematoxylin. DSL, Datura stramonium lectin; LCA, Lens culinaris agglutinin; PSA, Pisum sativum agglutinin; STL, Solanum tuberosum lectin; s-WGA, succinylated-wheat germ agglutinin. Scale bars = 50 μm.
그림 Fig. 5. Lectin histochemistry with galactose/N-acetylgalactosamine, complex type N-glycans (complex oligosaccharides), and fucosebinding lectins in the VSE of Korean native cattle. (A-F) The reactivities of galactose/N-acetylgalactosamine-binding lectins, including VVA (A, B), DBA (C, D), and PNA (E, F) in both neonatal (A, C, E) and adult VSE (B, D, F). (G, H) The reactivity of complex type N-glycans- binding lectin PHA-L in both neonatal (G) and adult VSE (H). I, J: The reactivity of fucose-binding lectin UEA-I in both neonatal (I) and adult VSE (J). Counterstained with hematoxylin. DBA, Dolichos biflorus agglutinin; PHL-L, Phaseolus vulgaris L; PNA, Arachis hypogaea (peanut) agglutinin; UEA-I, Ulex europaeus I; VVA, Vicia villosa agglutinin. Scale bars = 50 μm.
표 Table 4. Lectin-binding pattern in the vomeronasal non-sensory epithelium (VNSE) of Korean native cattle
그림 Fig. 6. Lectin histochemistry with N-acetylglucosamine and mannose-binding lectins in the VNSE of Korean native cattle. (A-F) The reactivities of N-acetylglucosamine-binding lectins, including s-WGA (A, B), DSL (C, D), and STL (E, F) in both neonatal (A, C, E) and adult VNSE (B, D, F). G-J: The reactivities of mannose-binding lectins, including LCA (G, H) and PSA (I, J) in both neonatal (G, I) and adult VNSE (I, J). Counterstained with hematoxylin. DSL, Datura stramonium lectin; LCA, Lens culinaris agglutinin; PSA, Pisum sativum agglutinin; STL, Solanum tuberosum lectin; s-WGA, succinylated-wheat germ agglutinin. Scale bars = 50 μm.
그림 Fig. 7. Lectin histochemistry with galactose/N-acetylgalactosamine, complex type N-glycans (complex oligosaccharides), and fucosebinding lectins in the VNSE of Korean native cattle. (A-F) The reactivities of galactose/N-acetylgalactosamine-binding lectins, including BSL-I (A, B), VVA (C, D), and ECL (E, F) in both neonatal (A, C, E) and adult VNSE (B, D, F). (G, H) The reactivity of complex type N-glycans-binding lectin PHA-E in both neonatal (G) and adult VNSE (H). I, J: The reactivity of fucose-binding lectin UEA-I in both neonatal (I) and adult VNSE (J). Counterstained with hematoxylin. BSL-I, Bandeiraea simplicifolia lectin I; ECL, Erythrina cristagalli lectin; PHA-E, Phaseolus vulgaris E, UEA-I, Ulex europaeus I; VVA, Vicia villosa agglutinin. Scale bars = 50 μm.

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