[논문]Cloning and characterization of a cDNA encoding a paired box protein, PAX7, from black sea bream, Acanthopagrus schlegelii

Choi, Jae Hoon; Han, Dan Hee; Gong, Seung Pyo

doi:10.12750/jarb.36.4.314

Cloning and characterization of a cDNA encoding a paired box protein, PAX7, from black sea bream, Acanthopagrus schlegelii 원문보기

Journal of animal reproduction and biotechnology = 한국동물생명공학회지, v.36 no.4, 2021년, pp.314 - 322

Choi, Jae Hoon (Department of Fisheries Biology, Pukyong National University) , Han, Dan Hee (Department of Fisheries Biology, Pukyong National University) , Gong, Seung Pyo (Department of Fisheries Biology, Pukyong National University)

Abstract ▼ AI-Helper

Paired box protein, PAX7, is a key molecule for the specification, maintenance and skeletal muscle regeneration of muscle satellite cells. In this study, we identified and characterized the cDNA and amino acid sequences of PAX7 from black sea bream (Acanthopagrus schlegelii) via molecular cloning and sequence analysis. A. schlegelii PAX7 cDNA was comprised of 1,524 bp encoding 507 amino acids and multiple sequence alignment analysis of the translated amino acids showed that it contained three domains including paired DNA-binding domain, homeobox domain and OAR domain which were well conserved across various animal species investigated. Pairwise Sequence Alignment indicated that A. schlegelii PAX7 had the same amino acid sequences with that of yellowfin seabream (A. latus) and 99.8% identity and similarity with that of gilt-head bream (Sparus aurata). Molecular phylogenetic analysis confirmed that A. schlegelii PAX7 formed a monophyletic group with those of teleost and most closely related with those of the fish that belong to Sparidae family including A. latus and S. aurata. In the investigation of its tissue specific mRNA expression, the expression was specifically identified in skeletal muscle tissue and a weak expression was also shown in gonad tissue. The cultured cells derived from skeletal muscle tissues expressed PAX7 mRNA at early passage but the expression was not observed after several times of subculture.

주제어

표/그림 (7)

표 Table 1. Information of primers used in this study
그림 Fig. 1. cDNA and deduced amino acid sequences of black sea bream (Acanthopagrus schlegelii ) PAX7. Numbers on left and right of figure indicate the number of nucleotides. One letter amino acid codes were presented beneath each of triple codons in nucleotide sequences. Asterisk indicates the stop codon.
그림 Fig. 2. Multiple sequence alignment analysis of PAX7 amino acid sequences of Acanthopagrus schlegelii with those of other teleost, avian, and mammalian species. The result was based on the following sequence data: A. latus (XP_036957057.1), Sparus aurata (XP_030276716.1), Oncorhynchus mykiss (NP_001245266.1), Danio rerio (NP_571400.1), Oryzias latipes (XP_011473129.1), O. melastigma (XP_024125911.1), Gallus gallus (NP_990396), Meleagris gallopavo (ADA61179), Homo sapiens (AAI21167.1), Mus musculus (NP_035169.1). Yellow line boxes (35-167 a.a.) indicate the predicted paired DNA-binding domain, green line box (219-279 a.a.) in- -dicates the predicted homeobox domain and blue line box (481-493 a.a.) indicates the predicted OAR domain in A. schlegelii PAX7 amino acid sequences. Black shading: identical sequences; gray shading: somewhat similar sequences (threshold 80%).
그림 Fig. 3. A phylogenetic tree based on amino acid sequences of PAX7 from black sea bream (Acanthopagrus schlegelii ), other teleost, avian, and mammalian species. Phylogenetic tree was constructed by neighbor-joining methods. Numbers on each node are bootstrap values from 1,000 replicates. Avian and mammalian species were used as the outgroups. The scale bar indicates a branch length of 0.02.
그림 Fig. 4. Evaluation of tissue-specific expression of PAX7 mRNA. cDNAs prepared from nine tissues including muscle, heart, gill, intestine, spleen, fin, liver, brain, and gonad were subjected to RT-PCR analysis using PAX7 primers. GAPDH was used as an internal control. The expression of PAX7 mRNA was observed in muscle and gonad tissues.
표 Table 2. Percentages of identities and similarities of PAX7 amino acid sequences with other animal species
그림 Fig. 5. Evaluation of PAX7 mRNA expression in the cultured cells derived from black sea bream (Acanthopagrus schlegelii) skeletal muscle tissues. (A) Morphology of muscle-derived cultured cells at passage 3 and 11. (B) PAX7 mRNA expression in the cultured cells. The cells at two different passages (passage 3 and 11) were subjected to RT-PCR analysis using PAX7 primers. GAPDH was used as an internal control. The cells expressed PAX7 at passage 3 but did not express at passage 11.

참고문헌 (32)

Aloisio GM, Nakada Y, Saatcioglu HD, Pena CG, Baker MD, Tarnawa ED, Mukherjee J, Manjunath H, Bugde A, Sengupta AL, Amatruda JF, Cuevas I, Castrillon DH. 2014. PAX7 expression defines germline stem cells in the adult testis. J. Clin. Invest. 124:3929-3944.

상세보기
Berberoglu MA, Gallagher TL, Morrow ZT, Talbot JC, Hromowyk KJ, Tenente IM, Amacher SL. 2017. Satellite-like cells contribute to pax7-dependent skeletal muscle repair in adult zebrafish. Dev. Biol. 424:162-180.

상세보기
Bischoff R and Heintz C. 1994. Enhancement of skeletal muscle regeneration. Dev. Dyn. 201:41-54.

상세보기
Brzoska E, Przewozniak M, Grabowska I, Moraczewski J. 2009. Pax3 and Pax7 expression during myoblast differentiation in vitro and fast and slow muscle regeneration in vivo. Cell Biol. Int. 33:483-492.

상세보기
Chapalamadugu KC, Murdoch BM, Robison BD, Murdoch GK. 2015. Oncorhynchus mykiss pax7 sequence variations with comparative analyses against other teleost species. Springerplus 4:263.

상세보기
Choi KH, Yoon JW, Kim M, Jeong J, Ryu M, Park S, Lee CK. 2020. Optimization of culture conditions for maintaining pig muscle stem cells in vitro. Food Sci. Anim. Resour. 40:659-667.

원문보기 상세보기
Ding S, Wang F, Liu Y, Li S, Hu P. 2017. Characterization and isolation of highly purified porcine satellite cells. Cell Death Discov. 3:17003.

상세보기
Du X, Wu S, Wei Y, Yu X, Ma F, Zhai Y, Yang D, Zhang M, Liu W, Zhu H, Wu J, Liao M, Li N, Bai C, Hua J. 2021. PAX7 promotes CD49f-positive dairy goat spermatogonial stem cells' self-renewal. J. Cell. Physiol. 236:1481-1493.

상세보기
Dumont NA, Rudnicki MA. 2015. Intrinsic and extrinsic mechanisms regulating satellite cell function. Development 142:1572-1581.

상세보기
Fan Q, Li D, Cai L, Qiu X, Zhao Z, Wu J, Lu Y. 2019. A novel mutation in the OAR domain of PITX3 associated with congenital posterior subcapsular cataract. BMC Med. Genet. 20:42.

상세보기
Codina M, Capilla E, Jimenez-Amilburu V, Navarro I, Du SJ, Gutierrez J; Garcia de la serrana D. 2014. Characterisation and expression of myogenesis regulatory factors during in vitro myoblast development and in vivo fasting in the gilthead sea bream (Sparus aurata). Comp. Biochem. Physiol. A Mol. Integr. Physiol. 167:90-99.

상세보기
Halevy O, Piestun Y, Allouh MZ, Rosser BW, Rinkevich Y, Reshef R, Rozenboim I, Yablonka-Reuveni Z. 2004. Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewal. Dev. Dyn. 231:489-502.

상세보기
Huang JD, Chang CF. 2002. The morphology of gonadal tissue and male germ cells in the protandrous black porgy, Acanthopagrus schlegeli. Zool. Stud. 41:216-227.

상세보기
Jun S and Desplan C. 1996. Cooperative interactions between paired domain and homeodomain. Development 122:2639-2650.

상세보기
Kwon HC, Zhang CI, Shin YJ, Kim KH, Seo YI. 2009. Maturation and spawning of black seabream Acanthopagrus schlegeli in the southern sea of Korea. Korean J. Ichthyol. 21:93-99.
Mayran A, Drouin J. 2015. Pax factors in transcription and epigenetic remodelling. Semin. Cell Dev. Biol. 44:135-144.

상세보기
Montarras D, Buckingham M. 2013. Lying low but ready for action: the quiescent muscle satellite cell. FEBS J. 280:4036-4050.

상세보기
Nicklas S, Otto A, Wu X, Miller P, Stelzer S, Wen Y, Kuang S, Wrogemann K, Patel K, Schwamborn JC. 2012. TRIM32 regulates skeletal muscle stem cell differentiation and is necessary for normal adult muscle regeneration. PLoS One 7:e30445.

상세보기
Pawlikowski B, Lee L, Kramer RH. 2009. Analysis of human muscle stem cells reveals a differentiation-resistant progenitor cell population expressing Pax7 capable of self-renewal. Dev. Dyn. 238:138-149.

상세보기
Pelletier A, Mayran A, Gouhier A, Omichinski JG, Drouin J. 2021. Pax7 pioneer factor action requires both paired and homeo DNA binding domains. Nucleic Acids Res. 49:7424-7436.

상세보기
Ramirez-Espinosa JJ, Gonzalez-Davalos L, Shimada A, Pina E, Mora O. 2016. Bovine (Bos taurus) bone marrow mesenchymal cell differentiation to adipogenic and myogenic lineages. Cells Tissues Organs 201:51-64.

상세보기
Rudnicki MA and Jaenisch R. 1995. The MyoD family of transcription factors and skeletal myogenesis. Bioessays 17:203-209.

상세보기
Seger C, Hargrave M, Wang X, Chai RJ, Ingham PW. 2011. Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease. Dev. Dyn. 240:2440-2451.

상세보기
Shi X and Garry DJ. 2006. Muscle stem cells in development, regeneration, and disease. Genes Dev. 20:1692-1708.

상세보기
Underhill DA and Gros P. 1997. The paired-domain regulates DNA binding by the homeodomain within the intact Pax-3 protein. J. Biol. Chem. 272:14175-14182.

상세보기
von Maltzahn J, Jones AE, Rudnicki MA. 2013. Pax7 is critical for the normal function of satellite cells in adult skeletal muscle. Proc. Natl. Acad. Sci. U. S. A. 110:16474-16479.

상세보기
Wang L, Zhang W, Gladstone S, Ng WK, Shao Q. 2019. Effects of isoenergetic diets with varying protein and lipid levels on the growth, feed utilization, metabolic enzymes activities, antioxidative status and serum biochemical parameters of black sea bream (Acanthopagrus schlegelii). Aquaculture 513:734397.

상세보기
Weber CM, Martindale MQ, Unguez GA. 2012. Activation of Pax7-positive cells in a non-contractile tissue contributes to regeneration of myogenic tissues in the electric fish S. macrurus. PLoS One 7:e36819.

상세보기
Wu GC, Chang CF. 2021. Molecular and cellular regulation on sex change in hermaphroditic fish, with a special focus on protandrous black porgy, Acanthopagrus schlegelii. Mol. Cell. Endocrinol. 520:111069.

상세보기
Zammit P and Beauchamp J. 2001. The skeletal muscle satellite cell: stem cell or son of stem cell? Differentiation 68:193-204.

상세보기
Zaret KS and Carroll JS. 2011. Pioneer transcription factors: establishing competence for gene expression. Genes Dev. 25:2227-2241.

상세보기
Zhou F, Shao Q, Xiao J, Peng X, Ngandzali BO, Ng WK. 2011. Effects of dietary arginine and lysine levels on growth performance, nutrient utilization and tissue biochemical profile of black sea bream, Acanthopagrus schlegelii, fingerlings. Aquaculture 319:72-80.

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증