[논문]Effects of functional nutrients on chicken intestinal epithelial cells induced with oxidative stress

Hyun Woo Kim; Seung Yun Lee; Sun Jin Hur; Dong Yong Kil; Jong Hyuk Kim

doi:10.5187/jast.2023.e22

Effects of functional nutrients on chicken intestinal epithelial cells induced with oxidative stress 원문보기

Journal of animal science and technology : JAST, v.65 no.5, 2023년, pp.1040 - 1052

Hyun Woo Kim (Department of Animal Science and Technology, Chung-Ang University) , Seung Yun Lee (Department of Animal Science (BK21 Four), Institute of Agriculture Life Science, Gyeongsang National University) , Sun Jin Hur (Department of Animal Science and Technology, Chung-Ang University) , Dong Yong Kil (Department of Animal Science and Technology, Chung-Ang University) , Jong Hyuk Kim (Department of Animal Science, Chungbuk National University)

Abstract ▼ AI-Helper

The objective of this study was to investigate the protective effects of functional nutrients including various functional amino acids, vitamins, and minerals on chicken intestinal epithelial cells (cIECs) treated with oxidative stress. The cIECs were isolated from specific pathogen free eggs. Cells were exposed to 0 mM supplement (control), 20 mM threonine (Thr), 0.4 mM tryptophan (Trp), 1 mM glycine (Gly), 10 μM vitamin C (VC), 40 μM vitamin E (VE), 5 μM vitamin A (VA), 34 μM chromium (Cr), 0.42 μM selenium (Se), and 50 μM zinc (Zn) for 24 h with 6 replicates for each treatment. After 24 h, cells were further incubated with fresh culture medium (positive control, PC) or 1 mM H2O2 with different supplements (negative control, NC and each treatment). Oxidative stress was measured by cell proliferation, whereas tight junction barrier function was analyzed by fluorescein isothiocyanate (FITC)-dextran permeability and transepithelial electrical resistance (TEER). Results indicated that cell viability and TEER values were less (p < 0.05) in NC treatments with oxidative stress than in PC treatments. In addition, FITC-dextran values were greater (p < 0.05) in NC treatments with oxidative stress than in PC treatments. The supplementations of Thr, Trp, Gly, VC, and VE in cells treated with H2O2 showed greater (p < 0.05) cell viability than the supplementation of VA, Cr, Se, and Zn. The supplementations of Trp, Gly, VC, and Se in cells treated with H2O2 showed the least (p < 0.05) cellular permeability. In addition, the supplementation of Thr, VE, VA, Cr, and Zn in cells treated with H2O2 decreased (p < 0.05) cellular permeability. At 48 h, the supplementations of Thr, Trp, and Gly in cells treated with H2O2 showed the greatest (p < 0.05) TEER values among all treatments, and the supplementations of VC and VE in cells treated with H2O2 showed greater (p < 0.05) TEER values than the supplementations of VA, Cr, Se, and Zn in cells treated with H2O2. In conclusion, Thr, Trp, Gly, and VC supplements were effective in improving cell viability and intestinal barrier function of cIECs exposed to oxidative stress.

주제어

표/그림 (3)

그림 Fig. 1. Effects of functional feed additives on cell proliferation in chicken intestinal epithelial cells (cIECs) after oxidative stress. All data are presented as mean ± SE (n=6). ^a-eBars with different letters were significantly different (p < 0.05). PC, positive control; NC, negative control; Thr, threonine; Trp, tryptophan; Gly, glycine; VC, vitamin C; VE, vitamin E; VA, vitamin A; Cr, chromium; Se, selenium; Zn, zinc.
그림 Fig. 2. Effects of functional feed additives on fluorescein isothiocyanate (FITC)-dextran permeability of tight junction in chicken intestinal epithelial cells (cIECs) after oxidative stress. All data are presented as mean ± SE (n=6). ^a-eBars with different letters were significantly different (p < 0.05). PC, positive control; NC, negative control; Thr, threonine; Trp, tryptophan; Gly, glycine; VC, vitamin C; VE, vitamin E; VA, vitamin A; Cr, chromium; Se, selenium; Zn, zinc.
그림 Fig. 3. Effects of functional feed additives on trans-epithelial electrical resistance (TEER) of tight junction in chicken intestinal epithelial cells (cIECs) after oxidative stress. All data are presented as mean ± SE (n=6). ^a-fBars with different letters were significantly different (p < 0.05). PC, positive control; NC, negative control; Thr, threonine; Trp, tryptophan; Gly, glycine; VC, vitamin C; VE, vitamin E; VA, vitamin A; Cr, chromium; Se, selenium; Zn, zinc.

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성능/효과

In conclusion, all functional nutrients used in the current study improve cell viability and decrease intestinal permeability. Especially, Thr, Trp, Gly, and VC are more effective in improving cell viability and decreasing intestinal permeability of cIECs.

후속연구

Especially, Thr, Trp, Gly, and VC are more effective in improving cell viability and decreasing intestinal permeability of cIECs. The current experiment can provide the potential approach to screen various functional nutrients in vitro before conducting in vivo studies.
Therefore, the objective of the present experiment was to compare the protective effects of functional nutrients on cIECs treated with oxidative stress.

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표제어: PCR

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