[논문]한약처방 JSB가 구금스트레스로 우울증을 유발한 생쥐의 행동과 생체지표에 미치는 영향

신윤민; 임형택; 황은영; 김가나; 이원길; 김연경; 장희진; 최정준

doi:10.7231/jon.2021.32.1.025

한약처방 JSB가 구금스트레스로 우울증을 유발한 생쥐의 행동과 생체지표에 미치는 영향
Effects of JSB, a Korean Medicine, on Behaviors and Biological Factors in a Mouse Model of Depression Induced by Restraint Stress 원문보기

동의신경정신과학회지 = Journal of oriental neuropsychiatry, v.32 no.1, 2021년, pp.25 - 37

신윤민 (대전대학교 한의과대학 분자의학실험실) , 임형택 (자하연한의원) , 황은영 (자하연한의원) , 김가나 (자하연한의원) , 이원길 (대전대학교 한의과대학 분자의학실험실) , 김연경 (대전대학교 한의과대학 분자의학실험실) , 장희진 (대전대학교 한의과대학 분자의학실험실) , 최정준 (대전대학교 한의과대학 분자의학실험실)

Abstract ▼ AI-Helper

Objectives: Depression is a mood disorder showing low energy, loss of interest, anhedonia, and anxiety. It affects a patients' daily life. This disease is considered a social problem because the patient may commit suicide in extreme cases. JSB is composed of 12 Korean medicinal herbs. It has been prescribed to patients with depression. The objective of this study is to investigate anti-depressive effects of JSB on restraint stress-induced depression in a mouse model. Methods: Depression was induced by restraint stress. Mice were orally administered JSB at 10, 20, or 40 mg/kg for 14 days. Forced swimming test (FST) and open field test (OFT) were performed. Brain-derived neurotrophic factor (BDNF) mRNA level was measured by real time-PCR. Plasma levels of corticosterone and serotonin were measured by ELISA. Blood levels of AST and ALS were measured using a biochemical analyzer. Results: JSB treatment significantly reduced the immobility time in FST. BDNF mRNA level was increased by JSB treatment in the hippocampi of mice. Although the expression of TNF-α was also increased by JSB, such increase was not statistically significant. The increase of corticosterone level in plasma induced by restraint stress was significantly down-regulated by JSB. JSB reduced blood level of AST, but not ALT. Conclusions: JSB has a potential to manage depression, setting a foundation for clinical application of Korean medicine with safety.

주제어

표/그림 (8)

표 Table 1. The Herbal Composition of JSB
표 Table 2. Nucleotide Sequence of Primers
그림 Fig. 1. Schematic diagram of experiments. OFT: open field test, FST: forced swimming test.
그림 Fig. 2. Effects of JSB on depressive-like behaviors. Mice were orally administered with PBS, fluoxetine, or JSB (10, 20, 40 mg/kg) prior to restraint stress. Depression was induced by chronic restraint stress in C57BL/6 mice (n=6/group) for 14 consecutive days. (A) FST was performed and immobility duration time for 4 min was analyzed by video tracking system. (B) OFT was performed and the number of transition across asigned quadrant zones for 15 min was analyzed. Data are mean±SEM values. Nor: without restraint stress, Con: restraint stress with vehicle, PC: positive control (fluoxetine). ^##p＜0.01 vs Nor, *p＜0.05, **p＜0.05 vs Con.
그림 Fig. 3. Effects of JSB on expressions of BDNF and TNF-α in brain. Mice were orally administered with PBS, fluoxetine, or JSB (10, 20, 40 mg/kg) prior to restraint stress. Depression was induced by chronic restraint stress in C57BL/6 mice (n=6/group) for 14 consecutive days. Total mRNAs were isolated from hippocampus of mice and real-time PCR was performed using (A) BDNF and (B) TNF-α specific primer sets. Data are mean±SD values. Nor: without restraint stress, Con: restraint stress with vehicle, PC: positive control (fluoxetine). **p＜0.01 vs Con.
그림 Fig. 4. Effects of JSB on the levels of corticosterone and serotonin in plasma. Mice were orally administered with PBS, fluoxetine, or JSB (10, 20, 40 mg/kg) prior to restraint stress. Depression was induced by chronic restraint stress in C57BL/6 mice (n=6/group) for 14 consecutive days. The levels of (A) corticosterone and (B) serotonin in blood plasma were measured by ELISA kits. Data are mean±SEM values. Nor: without restraint stress, Con: restraint stress with vehicle, PC: positive control (fluoxetine). ^#p＜0.05 vs Nor, *p＜0.05 vs Con.
그림 Fig. 5. Effects of JSB on the level of blood glucose. Mice were orally administered with PBS, fluoxetine, or JSB (10, 20, 40 mg/kg) prior to restraint stress. Depression was induced by chronic restraint stress in C57BL/6 mice (n=6/group) for 14 consecutive days. The level of blood glucose was measured on (A) 7 days and (B) 14 days. Data are mean±SEM values. Nor: without restraint stress, Con: restraint stress with vehicle, PC: positive control (fluoxetine). *p＜0.05 vs Con.
그림 Fig. 6. Effects of JSB on the levels of ALT and AST in plasma. Mice were orally administered with PBS, fluoxetine, or JSB (10, 20, 40 mg/kg) prior to restraint stress. Depression was induced by chronic restraint stress in C57BL/6 mice (n=6/group) for 14 consecutive days. The levels of (A) ALT and (B) AST in blood plasma were measured by automatic biochemical analyzer. Data are mean±SEM values. Nor: without restraint stress, Con: restraint stress with vehicle, PC: positive control (fluoxetine). *p＜0.05 vs Con.

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표제어: PCR

동의어: Packet Collision Rate

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