Zhang, Chijian
(Hua An Tang Biotech Group Co., Ltd.)
,
Lu, Yongjie
(Hua An Tang Biotech Group Co., Ltd.)
,
Ai, Yong
(Hua An Tang Biotech Group Co., Ltd.)
,
Xu, Xian
(Hua An Tang Biotech Group Co., Ltd.)
,
Zhu, Siyang
(Hua An Tang Biotech Group Co., Ltd.)
,
Zhang, Bing
(Guangdong He Ji Biotech Co., Ltd.)
,
Tang, Minghui
(Hua An Tang Biotech Group Co., Ltd.)
,
Zhang, Lanyue
(School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology)
,
He, Tinggang
(Hua An Tang Biotech Group Co., Ltd.)
Glabridin, a compound of the flavonoid, has shown outstanding skin-whitening and anti-aging properties, but its water insolubility limits its wide application. Therefore, glabridin liposome (GL) has been developed to improve its poor bioavailability, while there are few studies to evaluate its ameli...
Glabridin, a compound of the flavonoid, has shown outstanding skin-whitening and anti-aging properties, but its water insolubility limits its wide application. Therefore, glabridin liposome (GL) has been developed to improve its poor bioavailability, while there are few studies to evaluate its amelioration of UVB- induced photoaging. This study is performed to investigate the amelioration of GL against UVB- induced cutaneous photoaging. The prepared GL has a spheroidal morphology with an average diameter of 200 nm. The GL shows lower cytotoxicity than glabridin, but it has a more effective role in inhibition of melanin. Moreover, the application of GL can effectively relieve UV radiation induced erythema and leathery skin, associated with the down-regulated expression of inflammatory cytokines (TNF-α, IL-6 and IL-10). Taken together, these results demonstrate that GL has potentials as topical therapeutic agents against UVB radiation induced skin damage through inhibiting inflammation.
Glabridin, a compound of the flavonoid, has shown outstanding skin-whitening and anti-aging properties, but its water insolubility limits its wide application. Therefore, glabridin liposome (GL) has been developed to improve its poor bioavailability, while there are few studies to evaluate its amelioration of UVB- induced photoaging. This study is performed to investigate the amelioration of GL against UVB- induced cutaneous photoaging. The prepared GL has a spheroidal morphology with an average diameter of 200 nm. The GL shows lower cytotoxicity than glabridin, but it has a more effective role in inhibition of melanin. Moreover, the application of GL can effectively relieve UV radiation induced erythema and leathery skin, associated with the down-regulated expression of inflammatory cytokines (TNF-α, IL-6 and IL-10). Taken together, these results demonstrate that GL has potentials as topical therapeutic agents against UVB radiation induced skin damage through inhibiting inflammation.
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제안 방법
There is a lack of systematic experiment to comparatively study the amelioration of glabridin (GB) and glabridin liposome (GL) on UVB radiation induced skin damage. In this study, GL is prepared by thin film dispersed method and an animal model of skin photoaging induced by UVB radiation is established to evaluate their amelioration performance. It is expected to provide a theoretical basis for the application of GB and GL in sunscreen and anti-aging products.
GB, GL, and BL were dissolved in ethanol at a concentration of 20, 40, or 60 mg/ml for the later use. The mice were randomly divided into nine groups with three mice, including model group (MC), solvent group (SC), control group (CON) and six sample groups (GB or GL). GB/GL-L, GB/GL-M and GB/GL-H represented as the concentration of 20, 40, and 60 mg/ml, respectively.
대상 데이터
The female nude mice (6-wk old, about 15 g body weight) bought from the Guangdong Provincial Laboratory Animal Center (approval number: SCXK [Guangzhou]-2018-0020). The mice were acclimated at 22℃ with a 12-h light-dark cycle for one week.
The sections were stained by hematoxylin and eosin (H&E) staining. The images were recorded by Olympus IX71 Digital Camera (Olympus, Japan) at 200× magnifications. The skin thickness was measured by digital analysis (ImageJ, USA), and the thickness referred to the distance from the basement membrane of the cell space to the bottom of cuticle.
데이터처리
All quantitative data were presented as mean value ± standard deviation. The value variance among groups were analyzed by One-way ANOVA. The p < 0.
이론/모형
Optical density (OD) of supernatant was measured at 475 nm by an ELISA reader. The protein content was determined by BCA method. Relative melanin content was calculated by the following equation:
성능/효과
The radiation dose result in erythema on mice dorsal skin was defined as one minimal erythema dose (1 MED), which was calculated to be approximately 100 mJ/cm2. The radiation dose was increased by 1 Med per week, from 1 Med to 3 Med, and remained at 3 Med until the end of experiment.
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