[논문]천연자(川練子)가 만성 역류성 식도염 흰쥐에 미치는 효과

이진아; 신미래; 최정원; 노성수

doi:10.6116/kjh.2021.36.3.1.

천연자(川練子)가 만성 역류성 식도염 흰쥐에 미치는 효과
Effect of Toosendan Fructus on Chronic Acid Reflux Esophagitis Rats 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.36 no.3, 2021년, pp.1 - 8

이진아 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 최정원 (대구한의대학교 한의과대학 본초약리학교실) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective : Reflux esophagitis (RE), one of gastroesophageal reflux disease (GERD), is a disease that causes inflammation due to reflux of stomach contents such as stomach acid and pepsin due to the unstable gastroesophageal sphincter, and is currently increasing worldwide. The currently used treatment for reflux esophagitis has various side effects. Therefore, in this study the effect of Toosendan Fructus extract on chronic acid reflux esophagitis in rats was evaluated in order to find a new treatment material for reflux treatment. Methods : After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks; Normal rats (Normal, n=8), chronic acid reflux esophagitis rats (Control, n=8), Toosendan Fructus 200 mg/kg body weight/day-treated chronic acid reflux esophagitis rats (TF, n=8). After, we were taken esophageal tissue and esophageal mucosa damage was identified, and analyzed the expression of NADPH oxidase, AP-1/MAPK-related proteins, and tight junction proteins by western blot in esophageal tissue. Results : Toosendan Fructus administration significantly protected the esophageal mucosal damage of reflux esophagitis. Also, Toosendan Fructus significantly reduced the expression of NADPH oxidases (NOX2 and p22phox) and AP-1/MAPK-related proteins (c-Fos, c-Jun, p-p38, p-ERK, and p-JNK). In addition, it significantly increased the expression of tight junction proteins (Occludin, Claudin-3, and Claudin-4). Conclusions : These results suggest that Toosendan Fructus reduced damage to the esophageal mucosa by protecting the esophageal mucosa by upregulating tight junctions proteins as well as inhibiting the AP-1/MAPK pathway through reducing NADPH oxidases expression.

주제어

표/그림 (7)

표 Table 1. Total Polyphenol and Total Flavonoid Contents of Toosendan Fructus
그림 Fig. 1. Surgical induction of chronic acid reflux esophagitis. A representative gross image; (A), esophageal ulcer ratio; (B). All data are expressed means±SD (n=8). Normal rats; Normal, chronic acid reflux esophagitis rats; Control, Toosendan Fructus 200 mg/kg body weight/day-treated chronic acid reflux esophagitis rats; TF. Significance: ###p < 0.001 vs. Normal group, **p < 0.01 vs. Control group.
그림 Fig. 2. Body weight change. All data are expressed means±SD (n=8). Normal rats; Normal, chronic acid reflux esophagitis rats; Control, Toosendan Fructus 200 mg/kg body weight/day-treated chronic acid reflux esophagitis rats; TF. Significance: ^##p < 0.01 vs. Normal group, ^¥¥p < 0.01,^¥¥¥p < 0.001 vs. 1 day.
표 Table 2. Changes in Body Weight and Food Intake
그림 Fig. 3. Expression of NADPH oxidase proteins in esophagus. All data are expressed means±SD (n=8). Normal rats; Normal, chronic acid reflux esophagitis rats; Control, Toosendan Fructus 200 mg/kg body weight/day-treated chronic acid reflux esophagitis rats; TF. Significance: ^##p < 0.01, ^###p < 0.001 vs. Normal group, ^**p < 0.01, ^***p < 0.001 vs. Control group.
그림 Fig. 4. Expression of AP-1/MAPK proteins in esophagus. All data are expressed means±SD (n=8). Normal rats; Normal, chronic acid reflux esophagitis rats; Control, Toosendan Fructus 200 mg/kg body weight/day-treated chronic acid reflux esophagitis rats; TF. Significance: ^##p < 0.01, ^###p < 0.001 vs. Normal group, ^**p < 0.01, ^***p < 0.001 vs. Control group.
그림 Fig. 5. Expression of tight junction proteins in esophagus. All data are expressed means±SD (n=8). Normal rats; Normal, chronic acid reflux esophagitis rats; Control, Toosendan Fructus 200 mg/kg body weight/day-treated chronic acid reflux esophagitis rats; TF. Significance: ^##p < 0.01, ^###p < 0.001 vs. Normal group, ^**p < 0.01, ^***p < 0.001 vs. Control group.

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