[논문]만성 역류성 식도염 모델에서 빈랑(檳榔)와 황련(黃連) 복합물의 보호 효과

이세희; 이진아; 신미래; 이지혜; 노성수

doi:10.22246/jikm.2021.42.1.11

만성 역류성 식도염 모델에서 빈랑(檳榔)와 황련(黃連) 복합물의 보호 효과
The Protective Effect of Arecae Semen and Coptidis Rhizoma in a Chronic Reflux Esophagitis Rat Model 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.42 no.1, 2021년, pp.11 - 24

이세희 (대구한의대학교 한의과대학 본초약리학교실) , 이진아 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 이지혜 (한국한의학연구원 한약자원연구센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective: Chronic reflux esophagitis (CRE), characterized by esophageal mucosa ulcer, is caused by continuous backflow of gastric acid and consequent inflammation due to unstable gastroesophageal sphincter. The aim of the present study was to clarify the effect of an Arecae Semen and Coptidis Rhizoma mixture (AC-mix) on CRE. Methods: CRE was surgically induced in SD rats with three experimental groups used: normal; CRE control; and CRE treatment (200 mg/kg AC-mix). Blood and esophageal tissue were collected after two weeks of drug administration. The anti-oxidant activity of the AC-mix was measured by total polyphenol and total flavonoid contents as well as by radical scavenging activity with protein levels evaluated using western blotting. Results: CRE damage to the esophageal mucosa was significantly reduced in the AC-mix group as compared with the controls, and administration of the AC-mix was seen to inhibit NF-κBp65 activity. Consequently, the inactivation of NF-κBp65 significantly inhibited inflammatory mediators such as COX-2 and iNOS. Moreover, the anti-oxidant enzyme HO-1 significantly increased through activation of the Nrf2-Keap1 pathway. Matrix metalloproteinase-2 (MMP-2), which can break down collagen from the basement membrane and extracellular matrix, was decreased following AC-mix treatment, and elevated levels of MMP-2 were regulated by its tissue inhibitor. Conclusions: These results show that AC-mix can alleviate esophageal mucosa ulcer though inhibition of the NF-κBp65 inflammatory pathway and enhancement of the anti-oxidant Nrf2-Keap1 pathway.

주제어

표/그림 (7)

표 Table 1. Total Polyphenol and Total Flavonoid Contents of AC-mix Extract
그림 Fig. 1. Scavenging activity (IC50) of AC-mix on DPPH and ABTS free radical. AC-mix : Areca Semen and Coptidis Rhizoma mixture extract All values are expressed mean±SEM of three replications.
그림 Fig. 2. The gross mucosal injury evaluation in esophagus tissue. A representative gross image after surgical induction; (A), the opened gross mucosal injury; (B), gross mucosal injury ratio; (C), Normal; normal rats, Control; chronic reflux esophagitis control rats, AC-mix; Areca Semen and Coptidis Rhizoma mixture extract 200 mg/kg body weight/day-treated chronic reflux esophagitis rats. Data are mean±SD(n=8). Significance: ^###p<0.001 vs. Normal and *p<0.05 vs. Control.
그림 Fig. 3. Effects of AC-mix on Nrf2, Keap1, and HO-1 antioxidant related protein expression. Normal; normal rats, Control; chronic reflux esophagitis control rats, AC-mix; Areca Semen and Coptidis Rhizoma mixture extract 200 mg/kg body weight/day-treated chronic reflux esophagitis rats. Data are mean±SD (n=8). Significance: ^#p<0.05, ^##p<0.01 vs. Normal and *p<0.05 vs. Control.
그림 Fig. 4. Effects of AC-mix on NF-κBp65 inflammation related protein expression. Normal; normal rats, Control; chronic reflux esophagitis control rats, AC-mix; Areca Semen and Coptidis Rhizoma mixture extract 200 mg/kg body weight/day-treated chronic reflux esophagitis rats. Data are mean±SD (n=8). Significance: ^###p<0.001 vs. Normal and **p<0.01 vs. Control.
그림 Fig. 5. Effects of AC-mix on iNOS and COX-2 inflammation related protein expressions. Normal; normal rats, Control; chronic reflux esophagitis control rats, AC-mix; Areca Semen and Coptidis Rhizoma mixture extract 200 mg/kg body weight/day-treated chronic reflux esophagitis rats. Data are mean±SD (n=8). Significance: ^##p<0.01 vs. Normal and *p<0.05, **p<0.01 vs. Control.
그림 Fig. 6. Effects of AC-mix on MMP-2, TIMP-1, and TIMP-2 protein expressions. Normal; normal rats, Control; chronic reflux esophagitis control rats, AC-mix; Areca Semen and Coptidis Rhizoma mixture extract 200 mg/kg body weight/day-treated chronic reflux esophagitis rats. Data are mean±SD (n=8). Significance: ^##p<0.01 vs. Normal and *p<0.05, **p<0.01 vs. Control.

참고문헌 (37)

Jeon SG, Rhee PL. Prevalence, Clinical Manifestations, and Treatment of Gastroesophageal Reflux Disease in Korea. The Korean Journal of Gastroenterology 2002;39(6):393-401.
Lee SH. A Comparative Study on the Effects of Pinellia ternata, Zingiber officinale and Sobanhatang on Reflux Esophagit. Sangji University Graduate School 2017.
Kethman W, Hawn M. New Approaches to Gastroesophageal Reflux Disease. Journal of Gastrointestinal Surgery 2017;16(6):1544-52.

상세보기
Jang MW, Lim SW. Experimental Study for Effect of Banhasasim-tang on Mice with Reflux Esophagitis. The journal of internal Korean medicine 2013;34(4):362-74.
Sandhu DS, Fass R. Current Trends in the Management of Gastroesophageal Reflux Disease. Gut Liver 2017;12(1):7-16.

상세보기
Jang HJ. Clinical characteristics of gastroesophageal reflux disease and the risk factors for erosive esophagitis. Gyeongsang National University Graduate School 2009.
Castell DO, Murray JA, Tutuian R, Orlando RC, Arnold R. Review article: the pathophysiology of gastro-oesophageal reflux disease - oesophageal manifestations. Alimentary Pharmacology & Therapeutics 2004;20(9):14-25.
Colin-Jones DG. Histamine-2-receptor antagonists in gastroesophageal reflux. Gut 1989;30(10):1305-8.

상세보기
DeVault KR, Castell DO. Updated guidelines for the diagnosis and treatment of gastroesophageal reflux disease. American Journal Gastroenterol 1999;94(6):1434-42.
Klinkenberg-Knol EC, Jansen JMBJ, Festen HPM, Meuwissen SG, Lamers CB. Double-blind multicentre comparison of omeprazole and ranitidine in the treatment of reflux esophagitis. Lancet 1987;329(8529):349-51.

상세보기
Spechler SJ. Comparison of medical and surgical therapy for complicated gastroesophageal reflux disease in Veteran. The New England Journal of Medicine 1992;326:786-92.

상세보기
Lee KS, Kim SH, Chun SH, Park SS, Park CS, Shin YS. Antimicrobial Activity of Areca catachu L. Extract of against Intestinal Pathogens. The Korean Society of Food Science and Nutrition 1998;11(1):36-40.
Kim SH, Roh SS, Lee JA, Shin MR, Lee AR, Koo JS, et al. Improving Effects on Rats with Chronic Acid Reflux Esophagitis Treated of Coptidis Rhizoma Extract. The Korea Journal of Herbology 2019;34(1):117-24.

원문보기 상세보기
Kim BW. Effects of Hwangryeun Extract on the Recovery of Liver Function in CCl4-exposed Rat. Journal of Physiology & Pathology in Korea Medicine 2011;25(6):1020-5.
Go YJ, Kim YE, Kim HN, Lee EH, Cho EB, Akhmadjon S, et al. Inhibition effect against elastase, collagenase, hyaluronidase and anti-oxidant activity of thinning Green ball apple. The Korean Society for Applied Biological Chemistry 2020;63(1):43-50.
Lister CE, Lancaster JE, Sutton KH, Walker JR. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. Journal of the science of food and agriculture 1994;64(2):155-61.

상세보기
Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 1958;181:1199-200.

상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorizationassay. Free Radical Biology and Medicine 1999;26(9-10):1231-7.

상세보기
Lee JA, Park HJ, Kim SH, Kim MJ, Kim KJ, Shin MR, et al. Evaluation of Evodiae Fructus Extract on the Chronic Acid Reflux Esophagitis in Rats. Kor J Herbol 2019;34(2):15-23.

원문보기 상세보기
Han CH, Lee JS, Lim HH, Oh YS, Yoon YK, Kim SG, et al. The Significance of Gastroesophageal Reflux Disease (GERD) Symptoms in Patients with Reflux Esophagitis. Journal of Neurogastroenterology and Motility 2000;6(2):196-205.
Giri AK, Rawat JK, Singh M, Gautam S, Kaithwas G. Effect of lycopene against gastroesophageal reflux disease in experimental animals. BioMed Central Complement Altern Med 2015;15:110.

상세보기
Choi SY, Kim JH, Lee JM, Lee SH, Cho EJ. Protective effect of Acer okamotoanum from oxidative stress in C6 glial cells. Journal of applied biological chemistry 2017;60(2):141-7.

원문보기 상세보기
Jaramillo MC, Zhang DD. The emerging role of the Nrf2-Keap1 signaling pathway in cancer. Genes & Development 2013;27(20):2179-91.

상세보기
Kobayashi M, Yamamoto M. Molecular mechanisms activating the Nrf2-Keap1 pathway of antioxidant gene regulation. Antioxidants & Redox Signaling 2005;7(3-4):385-94.

상세보기
Lim JS. Expression of Nrf2 Transcription Factor in Rat Kidney. Chonnam National University Graduate School 2009.
Li L, Grenard P, Nhieu JT, Julien B, Mallat A, Habib A, et al. Heme oxygenase-1 is an antifibrogenic protein in human hepatic myofibrobalsts. Gastroenterology 2003;125(2):460-9.

상세보기
Nakao A, Otterbein LE, Overhaus M, Sarady JK, Tsung A, Kimizuka K, et al. Biliverdin protects the functional integrity of a transplanted syngeneic small bowel. Gastroenterology 2004;127(2):595-606.

상세보기
Lee HS. Suppressive effect of chlorella on oxidative stress and NFkB activity. Inje University Graduate School 2003.
Funk CD. Prostaglandins and leukotrienes; Advances in eicosanoid biology. Science 2001;294(5548):1871-5.

상세보기
Shon DH, Choi DW, Kim MH. Improvement of anti-inflammation activity of Gardeniae fructus extract by the tratment of β-Glucisudase. Korean Society of Food Science and Technology 2012;44(3):331-6.

원문보기 상세보기
Hippeli S, Elstner EF. Inhibition of biochemical model reactions for inflammatory processes by plant extracts: a review on recent developments. Free Radical Research 1999;31(1):81-7.

상세보기
McCartney-Francis N, Allen JB, Mizel DE, Albina JE, Xie QW, Nathan CF, et al. 1993. Suppression of arthritis by an inhibitor of nitric oxide synthase. Journal of Experimental Medicine 1993;178(2):749-54.

상세보기
Kim JO, Lee CW, Kim EK, Lee SJ, Park NH, Kim HS, et al. Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. Journal Ethnopharmacol 2011;137(1):427-33.

상세보기
Hwang YP, Choi JH, Kim HG, Choi JM, Hwang SK, Chung YC, et al. Cultivated ginseng suppresses ultraviolet B-induced collagenase activation via mitogen-activated protein kinases and nuclear factor NFκB/activator protein-1-dependent signaling in human dermal fibroblasts. Nutrition Research 2012;32(6):428-38.

상세보기
Johnson MD, Kim HR, Chesler L, Tsao-Wu G, Bouck N, Polverini PJ. Inhibition of angiogenesis by tissue inhibitor of metalloproteinase. Journal of Cellular Physiology 1994;160(1):194-202.

상세보기
Kim SH, Roh SS, Lee JA, Shin MR, Lee AR, Koo JS, et al. Improving Effects on Rats with Chronic Acid Reflux Esophagitis Treated of Coptidis Rhizoma Extract. Kor J Herbol 2019;34(1):117-24.

원문보기 상세보기
Kim MY, Shin YO, Lee JY, Lee AR, Shin SH, Kwon OJ, et al. Improving Effect of a Combined Extract of Rhei Rhizoma and Glycyrrhizae Rhizoma through Anti-oxidative Stress in Reflux Esophagitis rats. Kor J Herbol 2015;30(4):37-44.

원문보기 상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증