[논문]16S rRNA 유전자 염기서열 분석에 기반한 국내 재배 오이의 상재균총 분석

서동우; 김승민; 이현열; 염수진; 정희곤

doi:10.9721/kjfst.2021.53.3.334

16S rRNA 유전자 염기서열 분석에 기반한 국내 재배 오이의 상재균총 분석
16S rRNA gene-based sequencing of cucumber (Cucumis sativus L.) microbiota cultivated in South Korea 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.3, 2021년, pp.334 - 343

서동우 (충남대학교 식품공학과) , 김승민 (한국방송통신대학교 생활과학부) , 이현열 (충남대학교 식품공학과) , 염수진 (충남대학교 식품공학과) , 정희곤 (충남대학교 식품공학과)

초록
AI-Helper

본 연구에서는 16S rRNA 염기서열 분석을 통하여 시설재배 오이 내 상재균총 군집 특성을 분석하였으며, 수확 시기 및 지역에 따른 상재균총에 대한 정보를 제공하였다. 상재균총 다양성 분석(α-diversity)의 경우 5월 시료에서 더 높은 수치의 Observed OTUs와 Chao1 index가 나타났다. PCoA (β-diversity)분석을 통해서 수확 시기에 따른 상재균총의 차이가 존재함을 확인하였다. Phylum 수준에서는 Proteobacteria, Firmicutes, Actinobacteria가 우점하였고, class 수준에서는 Gammaproteobacteria, Bacilli, Alphaproteobacteria, Actinobacteria가 주로 존재하였다. Genus 수준에서는 시기적인 요인이 주로 상재균총에 영향을 끼치는 것을 확인할 수 있었으며, 일부 지역적 요인의 영향도 관찰 되었다. 5월 시료에서는 Aureimonas, Escherichia, Microbacterium이 11월 시료에서는 Enterococcus, Pseudomonas, Rhizobium이 더 높은 비율을 차지하였다. 이외에도, Acinetobacter, Aerococcus, Aureimonas, Enterobacter, Enterococcus, Escherichia, Pantoea, Pseudomonas, Staphylococcus와 같이 잠재적인 위험성을 가지는 genus가 존재함을 확인하였다.

Abstract ▼ AI-Helper

Various vegetables, including cucumbers, have a high probability of foodborne illness because they are usually eaten raw. In this study, we analyzed the 16S rRNA gene sequences of the cucumber (Cucumis sativus L.) microbiota. The diversity indices of cucumber cultivated in May were higher than in cucumber cultivated in November. At the phylum level, Proteobacteria, Firmicutes, and Actinobacteria were predominant. The classes generally comprised Gammaproteobacteria, Bacilli, Alphaproteobacteria, and Actinobacteria. At the genus level, the proportions of Aureimonas, Escherichia, and Microbacterium in samples from May were relatively high, whereas Enterococcus, Pseudomonas, and Rhizobium accounted for a higher proportion in samples from November. Moreover, it is noteworthy that potential pathogenic genera such as Acinetobacter, Aerococcus, Aureimonas, Enterobacter, Enterococcus, Escherichia, Pantoea, Pseudomonas, and Staphylococcus were detected. Although further studies on the characteristics of potential pathogens are required, our results can be used to improve the food safety of vegetables.

주제어

표/그림 (5)

그림 Fig. 1. Comparison of microbial diversity in the cucumber collected at each sampling time and site. The microbial diversity from each cucumber sample is estimated by Chao1 diversity and Shannon diversity indices. Shapes indicate each sampling region [Cheonan (red), Jincheon (green), Gongju (blue))]. The midline signifies mean and error bar indicates the standard deviation (SD). The x-axis indicates different sampling time and site. The y-axis indicates the number of OTUs (A), Chao1 index (B) and Shannon index (C). The asterisk signifies statistical difference between groups (*p<0.05, **p<0.01, ***p<0.001). N.S. means that there is not significant difference between groups.
그림 Fig. 2. Comparison of total bacterial loads in the cucumber according to sampling time and site. The quantity of total bacteria (log CFU/g) was measured using quantitative real-time PCR. The asterisk signifies statistical difference between groups (*p<0.05, **p<0.01, ***p<0.001). Error bar indicates the standard deviation (SD).
그림 Fig. 3. UniFrac-based principal coordinate analysis (PCoA) according to sampling times and sites. Sampling sites are represented as color in the box. Dot circles mean cluster according to sampling time. The percentage contributions to the variance of the data from principal components 1,2 and 3 (PCo 1, PCo 2, PCo 3) are listed along axes representing them.
그림 Fig. 4. Comparison of taxonomic composition at phylum and class level. The legend only showed the phyla (A) and classes (B) that found more than 1% in at least one sample.
그림 Fig. 5. Analysis of microbiota composition in cucumber at the genus level. (A) The tendency of relative abundance at the genus level was represented through Spearman's rank correlation coefficient-based Heat map (Ch-Cheonan, J-JIncheon, G-Gongju). Colors mean proportion of each genus (>0.5% at least in one sample). (B) The relative abundances of potential pathogenic genera were compared according to sampling time. The asterisk signifies statistical difference between each groups (*p<0.05, **p<0.01, ***p<0.001).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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