[논문]배초향이 RAW 264.7의 염증인자 생성에 미치는 영향

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doi:10.6116/kjh.2022.37.1.41.

배초향이 RAW 264.7의 염증인자 생성에 미치는 영향
Agastache rugosa modulates productions of inflammatory mediators in RAW 264.7 stimulated by lipopolysaccharide 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.1, 2022년, pp.41 - 50

Abstract ▼ AI-Helper

Objectives : The aim of this study was to investigate the effect of water extract of Agastache rugosa (AR) on productions of inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. Methods : Cell viabilities were measured with MTT assay. The production of nitric oxide (NO) from RAW 264.7 cells was measured with Griess reagent assay. The production of cytokines in RAW 264.7 cells was measured with multiplex cytokine assay. Results : AR showed no cytotoxicity on RAW 264.7 cells. AR at concentrations of 25, 50, 100, and 200 ㎍/mL significantly inhibited NO production in LPS-stimulated RAW 264.7 cells. AR at concentrations of 50, 100, and 200 ㎍/mL significantly inhibited productions of TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells; AR at concentrations of 50 and 200 ㎍/mL significantly inhibited productions of RANTES (CCL5) in LPS-stimulated RAW 264.7 cells; AR at concentrations of 100 ㎍/mL significantly inhibited productions of macrophage inflammatory protein-1β in LPS-stimulated RAW 264.7 cells; AR at concentrations of 50, 100, and 200 ㎍/mL significantly increased productions of IP-10 (CXCL10) in LPS-stimulated RAW 264.7 cells; AR at concentrations of 100 and 200 ㎍/mL significantly increased MCP-1 (CCL-2) in LPS-stimulated RAW 264.7 cells; AR at concentrations of 50 and 100 ㎍/mL significantly increased productions of IL-10 in LPS-stimulated RAW 264.7 cells. Conclusions : AR might have immunomodulatory effects on productions of NO, cytokines, and chemokines in LPS-stimulated RAW 264.7 mouse macrophages.

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표/그림 (10)

그림 Figure 1. Effects of AR on cell viability of RAW 264.7 cells for 24 h incubation. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. AR25, AR50, AR100, and AR200 mean 25 μg/mL of AR, 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. # p < 0.05 vs. Nor.
그림 Figure 2. Effects of AR on Nitric Oxide production in LPSstimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR25, AR50, AR100, and AR200 mean 25 μg/mL of AR, 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 3. Effects of AR on TNF-α production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 4. Effects of AR on IL-1β production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 5. Effects of AR on RANTES production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 6. Effects of AR on MIP-1β production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 7. Effects of AR on IL-10 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 8. Effects of AR on MCP-1 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 9. Effects of AR on IP-10 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Figure 10. Effects of AR on the production of G-CSF, LIX, MIP-1α, MIP-2, VEGF, and IL-6 in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. AR, water extract of Agastache rugosa. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. AR50, AR100, and AR200 mean 50 μg/mL of AR, 100 μg/mL of AR, and 200 μg/mL of AR, respectively. IN means indomethacin (0.5 μM). # p < 0.05 vs. Nor; * p < 0.05 vs. Con.

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