[논문]괴화, 어성초, 익모초에서 효소 분해에 의한 항산화 활성 변화 연구

차배천

doi:10.15429/jkomor.2021.21.1.1

[국내논문] 괴화, 어성초, 익모초에서 효소 분해에 의한 항산화 활성 변화 연구
Study on the Change of Antioxidant Activity by Enzymatic Hydrolysis in Sophora japonica Linne, Houttuynia cordata Thunberg, Leonurus japonicus Houttuyn 원문보기

한방비만학회지 = Journal of Korean Medicine for Obesity Research, v.21 no.1, 2021년, pp.1 - 9

Abstract ▼ AI-Helper

Objectives: The enzymatic hydrolysis is one of the processing methods that improve its effectiveness on medicinal herbs. In this research, changes in ingredients and activity by enzymatic hydrolysis were studied. Methods: For this study, a carbohydrate hydrolase such as viscozyme, which converts glycosides to aglycone, was applied to induce constituent changes in Sophora japonica Linne, Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn. Changes in antioxidant activity were measured using the 1,1-diphenyl-2-picrylhydrazl (DPPH) method, and changes in ingredients were analyzed by high performance liquid chromatography. Results: As a result of enzymatic hydrolysis, the content of quercetin was increased from 1.26 mg/g to 29.66 mg/g in Sophora japonica Linne, from 0 mg/g to 0.66 mg/g in Houttuynia cordata Thunberg and from 0.43 mg/g to 0.71 mg/g in Leonurus japonicus Houttuyn. As a result of the antioxidant experimentation, the IC50 of Sophora japonica Linne decreased from 5 ug/ml (MeOH extract) and 9.1 ug/ml (EtOAc fraction) to 3.0 ug/ml, Houttuynia cordata Thunberg decreased from 15.6 ug/ml (MeOH extract) and 13.6 ug/ml (EtOAc fraction) to 11.2 ug/ml, and Leonurus japonicus Houttuyn decreased from 14.4 ug/ml (MeOH extract) and 12.6 ug/ml (EtOAc fraction) to 10.2 ug/ml. Conclusion: In conclusion, it was confirmed that glycoside rutin contained in the three medicinal herbs was changed to quercetin which is the aglycone, by the enzymatic hydrolysis using viscozyme. In terms of antioxidant activity, Sophora japonica Linne showed a significant antioxidant activity value that closes to the control group butylated hydroxyanisole. Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn showed a minor increase in antioxidant activity.

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표/그림 (7)

표 Table 1. HPLC Analysis Conditions
그림 Fig. 1. (A) HPLC chromatogram of rutin and quercetin, (B) HPLC chromatography of MeOH extract of Sophora japonica Linne, (C) HPLC chromatogram of viscozyme (2.5%, 24 hr) reaction product of Sophora japonica Linne, (D) HPLC chromatogram of viscozyme (5%, 72 hr) reaction product of Sophora japonica Linne. HPLC: high performance liquid chromatography.
그림 Fig. 2. (A) HPLC chromatogram of rutin and quercetin, (B) HPLC chromatography of MeOH extract of Houttuynia cordata Thunberg, (C) HPLC chromatogram of viscozyme (1%, 24 hr) reaction product of Houttuynia cordata Thunberg. HPLC: high performance liquid chromatography.
그림 Fig. 3. (A) HPLC chromatogram of rutin and quercetin, (B) HPLC chromatography of MeOH extract of Leonurus japonicus Houttuyn, (C) HPLC chromatogram of viscozyme (1%, 24 hr) reaction product of Leonurus japonicus Houttuyn. HPLC: high performance liquid chromatography.
표 Table 2. Calibration Curve Equations and R2 Values of Rutin and Quercetin
표 Table 3. The Change in Content of Rutin and Quercetin by Enzymatic Hydrolysis
표 Table 4. The Change of Radical Scavenging Activity by Enzymatic Hydrolysis

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