[논문]Lactobacillus casei KCTC 3109에 의한 감국 추출물의 항산화능의 변화

이자복; 최재영

doi:10.22156/cs4smb.2021.11.05.223

[국내논문] Lactobacillus casei KCTC 3109에 의한 감국 추출물의 항산화능의 변화
Changes in antioxidant activity of Chrysanthemum indicum L. extract by Lactobacillus casei KCTC 3109 원문보기

융합정보논문지 = Journal of Convergence for Information Technology, v.11 no.5, 2021년, pp.223 - 231

이자복 ((주)엘파운더) , 최재영 (연성대학교 호텔외식조리과 호텔조리전공)

초록
AI-Helper

유산균에 의한 감국 추출물(CIL)의 항산화능의 변화를 알아보기 위해 64%, 80% ethanol 로 추출한 농도별 CIL를 발효시켜 total phenolic contents (TPC), flavonoid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power (RP), linoleic acid 자동산화 저해활성의 변화를 확인해 보았다. TPC의 경우 3109, 3237에서 증가하였으며, flavonoid에서는 모든 균주에서 감소를 나타냈다. DPPH 측정에서는 64% CIL 발효 후 3074, 3109에서 증가하였으며, 80% CIL 발효에서는 모든 균주가 증가를 나타냈다. RP측정에서는 64%, 80% CIL에서 모든 균주에서 증가를 보였으며, linoleic acid 자동산화 저해활성에서는 모든 균주에서 감소를 나타냈다. 특히 3109 항산화능을 평가하는 DPPH와 RP 측정에서 다른 균주에 비해 전반적으로 높게 측정이 되었다. 결과적으로 4종의 유산균 중에서 3109가 발효과정을 통해서 CIL의 항산화 효능을 효과적으로 증대하는 것을 확인할 수 있었다.

Abstract ▼ AI-Helper

The antioxidant activity of Chrysanthemum indicum L. extract (CIL) was investigated by fermenting lactic acid bacteria with the CIL from 64% and 80% ethanol extraction and measuring the total phenolic contents (TPC), flavonoid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power (RP), and linoleic acid auto-oxidation inhibitory activity. CIL was confirmed to inhibit bacterial auto-oxidation. TPC was increased in strains 3109 and 3237, while flavonoid decreased in all strains. DPPH was increased in strains 3074 and 3109 fermented with 64% CIL and all the strains with 80% CIL. RP was increased and linoleic acid auto-oxidation inhibitory activity decreased in all the strains fermented with 64% or 80% CIL. Among the 4 strains, strain 3109 had the highest DPPH and RP; thus, it was most effective in increasing CIL's antioxidant efficacy through the fermentation process.

주제어

표/그림 (7)

표 Table 1. List of lactic acid bacteria
표 Table 2. Fermentation characteristics of the strains of lactic acid bacteria
그림 Fig. 1. The total phenolic contents of CIL fermented with lactic acid bacteria CIL, Chrysanthemum indicum L. extract; 3074, Lactobacillus paracasei subsp. tolerans; 3109, Lactobacillus casei; 3115, Lactococcus lactis subsp. lactis; 3237, Lactobacillus rhamnosus; The data are expressed as mean±SD. The significance of the difference was analyzed using one-way ANOVA followed by Dunnett's test. **p<0.01, ***p<0.001, comparison between the 64% CIL group; #p<0.05, ##p<0.01, comparison between 80% CIL group.
그림 Fig. 2. Contents of flavonoid of CIL fermented with lactic acid bacteria CIL, Chrysanthemum indicum L. extract; 3074, Lactobacillus paracasei subsp. tolerans; 3109, Lactobacillus casei; 3115, Lactococcus lactis subsp. lactis; 3237, Lactobacillus rhamnosus; The data are expressed as mean±SD. The significance of the difference was analyzed using one-way ANOVA followed by Dunnett's test. ***p<0.001, comparison between the 64% CIL group; ###p<0.001, comparison between 80% CIL group.
그림 Fig. 3. DPPH radical rate of CIL fermented with lactic acid bacteria CIL, Chrysanthemum indicum L. extract; 3074, Lactobacillus paracasei subsp. tolerans; 3109, Lactobacillus casei; 3115, Lactococcus lactis subsp. lactis; 3237, Lactobacillus rhamnosus; The data are expressed as mean±SD. The significance of the difference was analyzed using one-way ANOVA followed by Dunnett's test. **p<0.01, ***p<0.001, comparison between the 64% CIL group; ###p<0.01, comparison between 80% CIL group.
그림 Fig. 4. Reducing power of CIL fermented with lactic acid bacteria CIL, Chrysanthemum indicum L. extract; 3074, Lactobacillus paracasei subsp. tolerans; 3109, Lactobacillus casei; 3115, Lactococcus lactis subsp. lactis; 3237, Lactobacillus rhamnosus; The data are expressed as mean±SD. The significance of the difference was analyzed using one-way ANOVA followed by Dunnett's test. **p<0.01, comparison between the 64% CIL group; ##p<0.01, comparison between 80% CIL group.
그림 Fig. 5. Inhibition of linoleic acid auto-oxidation of CIL fermented with lactic acid bacteria CIL, Chrysanthemum indicum L. extract; 3074, Lactobacillus paracasei subsp. tolerans; 3109, Lactobacillus casei; 3115, Lactococcus lactis subsp. lactis; 3237, Lactobacillus rhamnosus; The data are expressed as mean±SD. The significance of the difference was analyzed using one-way ANOVA followed by Dunnett's test. ***p<0.001, comparison between the 64% CIL group; ###p<0.001, comparison between 80% CIL group.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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