[논문]사과 캘러스로부터 분리된 엑소좀-유사 Nanovesicles 의 피부 장벽 및 피부 노화 방지 개선 연구

서유리; 이광수; 강용원

doi:10.15230/scsk.2021.47.2.139

사과 캘러스로부터 분리된 엑소좀-유사 Nanovesicles 의 피부 장벽 및 피부 노화 방지 개선 연구
Study on Reinforcing Skin Barrier and Anti-aging of Exosome-like Nanovesicles Isolated from Malus domestica Fruit Callus 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.47 no.2, 2021년, pp.139 - 145

서유리 ((주)바이오솔루션) , 이광수 ((주)바이오솔루션) , 강용원 ((주)바이오솔루션)

초록
AI-Helper

식물 유래 exosome-like nanovesicles (plant-derived exosome-like nanovesicles, PELNs)은 다양한 생물학적 활성을 포함하고 높은 생체 적합성을 가지고 있다. 인체 내에서 PELNs은 세포 분화 및 증식 조절에 영향을 미칠 수 있어 여러 산업 분야에서 응용이 가능하다. 하지만, PELNs의 피부 생리적 기능에 대한 연구는 포유류 nanovesicles에 비해 미미한 실정이다. 본 연구에서는 사과 열매로부터 캘러스를 유도하고 exosome-like nanovesicles (Exosome-like nanovesicles isolated from Malus domestica (apple) fruit callus, ACELNs)를 분리하여 피부 장벽 및 피부 노화 개선에 대한 연구를 수행하였다. ACELNs의 수율은 6.42 × 10⁹ particles/mL이였으며, 입자 사이즈는 100 ~ 200 nm 범위로 감지되었다. 인간 유래 피부세포인 HDF cells과 HaCaT cells에서 세포 증식을 유도하였으며, 세포 독성 억제 효과를 보였다. 각질형성능이 유의하게 증가했으며, mRNA levels에서 COL1A1과 FBN1 발현을 증가시켰다. 또한, UVA 조사된 HDF cells에 대한 collagen 합성을 촉진시켰다. 이러한 결과들은 ACELNs가 피부장벽 개선 및 피부노화를 방지할 수 있는 소재로서 활용하기 우수한 소재로 사료된다.

Abstract ▼ AI-Helper

Plant-derived exosome-like nanovesicles (PELNs) are known to include various biological activities and possess high biocompatibility. Because PELNs can influence immune responses, cell differentiation, and proliferation regulation, they can be applied in multiple industries. However, the studies on the skin physiological of exosome-like nanovesicles derived from plant callus are insignificant compared to nanovesicles derived from mammalian cells. In this study, callus was induced from apple fruit (Malus domestica), and exosome-like nanovesicles (ACELNs) were isolated for improving skin barrier and anti-aging. The yield of ACELNs was 6.42 × 109 particles/mL, and the particle size was ranged from 100 to 200 nm. HDF cells and HaCaT cells were concentration-dependent, increased in cell, and decreased in cytotoxicity. The cornified envelope formation was significantly increased compared to the control group. The COL1A1 expression and the FBN1 expression in HDF cells were increased. In addition, the ACELNs promoted collagen biosynthesis in UVA-irradiated HDF cells. These results might be considered as potential materials that could improve skin barrier and prevent skin aging.

주제어

표/그림 (7)

그림 Figure 1. Microscope images of cell lysis by microfluidizer: (A) for 0 cycle, (B) 1 cycle, (C) 2 cycles, and (D) 3 cycles at 1,000 bar.
그림 Figure 2. Nanoparticle tracking analysis of exosome-like nanovesicles isolated from Malus domestica (apple) fruit callus (ACELNs). (A) Representative graph shows particle concentration and their size measurements. (B) The scattering distributions are presented from three consecutive 30 s runs for nanoparticles.
표 Table 1. Forward and Reverse Primer PCR Sequence for qRT-PCR
그림 Figure 3. Effect of ACELNs on the cell proliferation and inhibition of cytotoxicity. Cell proliferation of (A) HaCaT cells, and (B) HDF cells measured by the CCK-8 assay after treatment with different concentrations (0.001, 0.01, 0.02% ACELNs) for 48 h. Inhibition of cytotoxicity of (C) HaCaT cells, and (D) HDF cells measured by LDH assay. ***p < 0.001 versus a control group.
그림 Figure 4. Cornified envelope (CE) formation increasing rate by ACELNs treatment in HaCaT cells. ***p < 0.001 versus a control group.
그림 Figure 5. The expression levels of collagen type 1 (COL1A1), and fibrillin (FBN1) in HDF cells by qRT-PCR. ***p < 0.001 versus a control group.
그림 Figure 6. The effect of ACELNs on collagen biosynthesis in UVA-irradiated HDF cells. (A) The concentrations of collagen were measured via ELISA assay. (B) Protective effects of ACELNs on cytotoxicity of HDF cells against UVA-irradiated cell damage. ***p < 0.001 versus a control group.

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