[논문]흑효모를 이용한 참도박 발효 추출물의 항염 효과

부반빈; 이경은; 강상구

doi:10.15230/scsk.2021.47.2.123

흑효모를 이용한 참도박 발효 추출물의 항염 효과
Anti-inflammatory effects of Grateloupia elliptica Fermenting Extracts Using Aureobasidium pullulans 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.47 no.2, 2021년, pp.123 - 131

부반빈 (영남대학교 생명응용과학대학 생명공학과) , 이경은 (영남대학교 생명응용과학대학 생명공학과) , 강상구 (영남대학교 생명응용과학대학 생명공학과)

초록
AI-Helper

해양 홍조류 참도박(Grateloupia elliptica, G. elliptica)을 흑효모(Aureobasidium pullulans, A. pullulans)로 발효시킨 추출물들의 인간 피부각질세포주(HaCaT)에 대한 항염증 효과를 조사하였다. 흑효모로 발효한 참도박 추출물은 참도박 추출물에 비하여 총 폴리페놀(total polyphenol content)은 최대 2.7 배로 증가되었으며 총 플라보노이드(total flavonoid conten)는 최대 2.4 배 증가되었다. 인간 피부각질세포를 lipopolysaccharide (LPS) 또는 H₂O₂로 염증을 유도 한 후 참도박 추출물과 참도박 발효 추출물을 100 ㎍/mL 농도로 처리 한 결과 LPS 또는 H₂O₂ 만 처리한 세포군에 비하여 약 3 ~ 10% 정도 세포생존율이 증가되었다. 또한 염증 유발과 관련된 단백질 발현 변화를 조사한 결과 참도박 발효추출물이cyclooxygenase-2 및 70 kDa heat shock protein 의 발현을 현저히 감소시켰다. 따라서 흑효모로 발효한 참도박은 항염효과를 가진 피부수렴용 화장품 소재로 유용하다.

Abstract ▼ AI-Helper

In this study, we investigated the biological functions of Grateloupia elliptica (G. elliptica) fermented with Aureobasidium pullulans (A. pullulans). Total phenolic contents (TPC) of the hot-water extract of the fermented G. elliptica increased 2.7 folds than that of the non-fermented G. elliptica. Furthermore, total flavonoid contents of both the hot-water extract and the ethanol extract increased maximum 2.4 folds amounts than non-fermented G. elliptica extracts.HaCaT cells were induced inflammation treated with LPS (1 ㎍/mL) or H2O2 (1mM) and examined with 100 ㎍/mL of G. elliptica extracts. The extraction of the fermented G. elliptica increased HaCaT cell proliferation in the maximum 10% than non-fermented G. elliptica extraction. Furthermore, investigating changes in protein expression associated with inflammation resulted in a significant reduction in the expression of cyclooxygenase-2 and 70 kDa heat shock proetin. Conclusively, the extracts of G. elliptica fermented with A. pullulans have bioactive functions both anti-oxidant to protect environmental stresses and anti-inflammation activity. Hence, G. elliptica fermented with A. pullulans would be a good natural resource as bioactive ingredients for cosmetics. Therefore, G. elliptica fermented with A. pullulans is useful as a astringent material with anti-inflammatory skin.

주제어

표/그림 (7)

그림 Figure 1. Diagram for preparation of algae extracts and fermented algae extracts.
그림 Figure 2. The total phenolic content of G. elliptica water extract (GEWE), G. elliptica ethanol extract (GEEE), G. elliptica fermentation water extract (GEFWE), G. elliptica fermentation ethanol extract (GEFEE), Aureobasidium pullulans (A. pullulans).
그림 Figure 3. The total flavonoid content of G. elliptica water extract(GEWE), G. elliptica ethanol extract (GEEE), G. elliptica fermentationwater extract (GEFWE), G. elliptica fermentation ethanol extract(GEFEE), Aureobasidium pullulans (A. pullulans).
그림 Figure 4. Cell viability and proliferation in HaCaT keratinocyte cells treated with LPS (1 μg/mL) for 24 h and 100 μg/mL of G. elliptica water extract (GEWE), G. elliptica ethanol extract (GEEE), G. elliptica fermentation water extract (GEFWE), G. elliptica fermentation ethanol extract (GEFEE), Aureobasidium pullulans (A. pullulans) for 72 h. Statistical significance was determined with respect to the control. Control was not treated with any materials (*p < 0.05 vs control).
그림 Figure 5. Cell viability and proliferation in HaCaT keratinocyte cells treated with H₂O₂ (1 mM) for 24 h and 100 μg/mL of G. elliptica water extract (GEWE), G. elliptica ethanol extract (GEEE), G. elliptica fermentation water extract (GEFWE), G. elliptica fermentation ethanol extract (GEFEE), Aureobasidium pullulans (A. pullulans) for 72 h. Statistical significance was determined with respect to the control. Control was not treated with any materials (*p < 0.05 vs control).
그림 Figure 6. Western blot measurements of COX-2, Hsp70 expressionso in HaCaT keratinocyte cells treated with LPS (1 μg/mL) for 24 h and 100 μg/mL of G. elliptica water extract (GEWE), G. elliptica ethanol extract (GEEE), G. elliptica fermentation water extract (GEFWE), G. elliptica fermentation ethanol extract (GEFEE), Aureobasidium pullulans (A. pullulans) for 72 h. Statistical significance was determined with respect to the control. Control was not treated with any materials (*p < 0.05 vs control). The presented western blot figure was selected from three times repeated blotting experiments.
그림 Figure 7. Western blot measurements of COX-2, Hsp70 expressionso in HaCaT keratinocyte cells treated with H₂O₂ (1 mM) for 24 h and 100 μg/mL of G. elliptica water extract (GEWE), G. elliptica ethanol extract (GEEE), G. elliptica fermentation water extract (GEFWE), G. elliptica fermentation ethanol extract (GEFEE), Aureobasidium pullulans (A. pullulans) for 72 h. Statistical significance was determined with respect to the control. Control was not treated with any materials (*p < 0.05 vs control). The presented western blot figure was selected from three times repeated blotting experiments.

참고문헌 (26)

R. Lee, H. Kim, J. M. Yu, Y. H. Cho, D. I. Kim, Y. Shin, Y. Cho, O. J. Kwon, and B. An, Anti-inflammatory effect of Polygonum multiflorum extraction in activated RAW 264.7 cells with lipopolysaccharide, Korean J. Food Preserv., 21(5), 740 (2014).

원문보기 상세보기
K. E. Lee, J. J. Nam, S. M. Kim, H. K. Kim, S. J. Moon, and J. K. Youm, Anti-Inflammatory effects of the mixture of Sorbus commixta, Urtica dioica, Phyllostachys nigra, and Rhus semialata gall extracts on LPS-induced inflammation in HaCaT cells, J. Soc. Cosmet. Sci. Korea, 40(1), 45 (2014).

원문보기 상세보기
L. Ferrero-Miliani, O. H. Nielsen, P. S. Andersen, and S. E. Girardin, Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation, Clin Exp Immunol, 147(2), 227 (2007).

상세보기
N. Yahfoufi, N. Alsadi, M. Jambi, and C. Matar, The immunomodulatory and anti-inflammatory role of polyphenols, Nutrients, 10(11), 1618 (2018).

상세보기
J. N. Barker, R. S . Mitra, C. E. Griffiths, V. M. Dixit, and B. J. Nickoloff, Keratinocytes as initiators of inflammation, Lancet., 337(8735), 211 (1991).

상세보기
B. Zhao, R. Li, F. Yang, F. Yu, N. Xu, F. Zhang, X. Ge and J. Du, LPS -induced vitamin D receptor decrease in oral keratinocytes is associated with oral lichen planus, Sci Rep., 8(1), 763 (2018).
Y. He, B. G. Kim, H. E. Kim, Q. Chu, S. Shi, G. Ma, Y. Kim, O. S . Kim, and O. J. Kim, The protective role of feruloylserotonin in LPS-induced HaCaT cells, Molecules, 24(17), 3064 (2019).

상세보기
B. Bertani and N. Ruiz, Function and biogenesis of lipopolysaccharides, EcoSal Plus, 8(1), 10 (2018).
J. Radons, The human HSP70 family of chaperones: where do we stand?, Cell Stress Chaperones, 21(3), 379 (2016).

상세보기
T. J. Borges, L. Wieten, M. J. C. V. Herwijnen, F. Broere, R. V. D. Zee, C. Bonorino, and W. V. Eden, The anti-inflammatory mechanisms of Hsp70, Front Immunol, 3, 95 (2012).
N. Y. Bae, M. J. Kim, K. B. W. R. Kim, N. K. Ahn, Y. U. Choi, J. H. Park, S. H. Park, and D. H. Ahn, Anti-inflammatory effect of ethanol extract from Grateloupia elliptica holmes on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells and mice ears, J. Korean Soc. Food Sci. Nutr., 44(8), 1128 (2015).

원문보기 상세보기
K. Y. Kim, K. A. Nam, H. Kurihara, and S. M. Kim, Potent α-glucosidase inhibitors purified from the red alga Grateloupia elliptica, Phytochemistry, 69(16), 2820 (2008).

상세보기
J. Kang, S. C. Kim, S. C. Han, H. J. Hong, Y. J. Jeon, B. Kim, Y. S. Koh, E. S. Yoo, and H. K. Kang, Hair-loss preventing effect of Grateloupia elliptica, Biomol. Ther (Seoul), 20(1), 118 (2012).

원문보기 상세보기
M. L. Cho, G. M. Park, S. N. Kim, T. Amna, S. Lee, and W. S. Shin, Glioblastoma-specific anticancer activity of pheophorbide a from the edible red seaweed Grateloupia elliptica, J. Microbiol. Biotechnol., 24(3), 346 (2014).

원문보기 상세보기
Z. Chi, F. Wang, Z. Chi, L. Yue, G. Liu, and T. Zang, Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast, Appl. Microbiol. Biotechnol., 82(5), 793 (2009).

상세보기
H. Y. Choi, J. D. Kim, and M. Y. Park, A 4 week Randomized, Double-blind human trial to compare the efficacy and safety of Aureobasidium pullulans cultured solution and placebo on improvement of immune in subjects, Korean Journal of Oriental Medicine., 15(3), 83 (2009).
E. K. Park, S . M. Kang, and M. H. Leem, A study on the variation of skin moisture, oil(sebum), melanin and erythema index after application of β-glucan, Kor J Aesthet Cosmetol., 1(3), 83 (2003)
Y. E. Kim, M. A. Yeo, J. H. Han, J. M. Lee, S. K. Jung, H. A. Jeong, S. H. Kim, and J. E. Lee, Aureobasidium pullulans ferment of black tea for anti-oxidation, whitening and anti-wrinkle effects, J. Soc. Cosmet. Sci. Korea, 43(3), 265 (2017).

원문보기 상세보기
Korea Patent 10-0138716 (2016).
C. Cheng, Y. Zhou, M. Lin, P. Wei, and S. T. Yang, Polymalic acid fermentation by Aureobasidium pullulans for malic acid production from soybean hull and soy molasses: Fermentation kinetics and economic analysis, Bioresour. Technol., 223, 166 (2017).

상세보기
C. An, S . J. Ma, F. Chang, and W. J. Xue, Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose, Braz. J. Microbiol., 48(1), 180 (2017).

상세보기
S. Suraiya, Y. B. Choi, H. D. Park, W. J. Jang, H. H. Lee, and I. S. Kong, Saccharina japonica fermented by Monascus spp. inhibit adipogenic differentiation and gene expression analyzed by real-time PCR (Q-PCR) in 3T3-L1 cell, J. Funct. Foods, 55, 371 (2019).

상세보기
Korea Patent 10-1647049 (2016).
Korea Patent 10-0893350 (2009).
A. Sushant, B. K. Manoj, D. Krisha, K. Puspa, G. Roshani, and K. Niranjan, Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western nepal, Plants (Basel), 8(4), 96 (2019).

상세보기
V. V. Vu, K. E. Lee, and S . G. Kang, Evaluation of antioxidant, tyrosinase and collagenase inhibitory of Grateloupia elliptica extracts after Aureobasidium pullulans fermentation, J. Soc. Cosmet. Sci. Korea, 46(1), 1 (2020).

원문보기 상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증