[논문]유산균 유래 엑소좀 유사 나노베지클의 피부 장벽 개선 효과

왕혜수; 이광수; 강용원

doi:10.15230/scsk.2021.47.2.171

유산균 유래 엑소좀 유사 나노베지클의 피부 장벽 개선 효과
Skin Barrier Improvement Effect of Exosomal Nanovesicles Derived from Lactic Acid Bacteria 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.47 no.2, 2021년, pp.171 - 178

왕혜수 ((주)바이오솔루션) , 이광수 ((주)바이오솔루션) , 강용원 ((주)바이오솔루션)

초록
AI-Helper

본 연구에서는 프로바이오틱스 유래 엑소좀 유사 나노베지클을 분리하고, 피부에 대한 여러 가지 생리활성을 평가했다. 프로바이오틱스의 한 종인 Lactococcus lactis subsp. lactis (LL)를 배양하고 고압균질기와 한외여과를 통해 70 ~ 200 nm 크기를 갖는 LL 유래 엑소좀 유사 나노베지클(LVs)을 분리했다. 나노입자추적분석 결과 1.81 × 10¹¹ particles/mL로 나타났다. LVs를 섬유아세포와 피부각질세포에 처리하여 피부 주름과 장벽 개선과 관련된 효능을 확인했다. 우선 섬유아세포에서 fibrillin (FBN1) 유전자 발현량이 23%, 피부각질세포에서 fibronectin (FN1)과 filaggrin (FGN) 유전자 발현량이 각각 65%, 400% 증가했다. 그리고 각질형성능은 대조군 대비 30% 증가함을 확인할 수 있었다. 또한, UV 조사한 피부각질세포에 LVs를 처리했을 때 collagen type I alpha 1 (COL1A1)이 대조군 대비 약 83% 증가하는 결과를 보여주었다. 이로써 프로바이오틱스 유래 엑소좀 유사 나노베지클은 장벽 개선과 관련하여 화장품 및 의약품 소재로 이용할 수 있음을 확인했다.

Abstract ▼ AI-Helper

In this study, exosomal-like nano-vesicles derived from probiotics were isolated and various physiological activities were evaluated on the skin. This study show that Lactococcus lactis subsp. lactis (LL) are incubated, and then isolated LL derived exosomal nanovesicles (LVs) at the range of 70 ~ 200 nm by high-pressure homogenizer and ultrafiltration. The vesicle numbers were an average of 1.81 × 1011 particles/mL. This study finds out the bacterial nanovesicles' beneficial effect on the skin. Fibrillin (FBN1) gene expression increased by 23% in fibroblast cells. Fibronectin (FN1) and filaggrin (FLG) gene expression increased by 65% and 400% in keratinocytes. We could see that cornified envelope (CE) formation ability was increased by 30% compared to the control group. Furthermore, collagen type I alpha 1 (COL1A1) protein expression increased by 83% compared to the UV-irradiated control group. These results suggest that LVs could help skin barrier improvement and used as an ingredient for cosmetics or pharmaceuticals.

주제어

표/그림 (9)

표 Table 1. Forward and Reverse Primer PCR Sequence for qRT-PCR
그림 Figure 1. Characterization of exosomal nanovesicles isolated from Lactococcus lactis subsp. lactis ( L L) (A) Representative graph shows particle concentration and their size measurements. (B) The scattering distributions are presented from three consecutive 30 s runs for nanoparticles.
표 Table 2. Results of Nanoparticle Tracking Analysis
그림 Figure 2. E ffect o f L Vs o n the cell v iability. ( A) H aCaT, (B) HDF. (-); non-treated, lysis; lysis buffer treated, LVs; LVs treated. The results are expressed as the mean ± SD (N = 3). ***p < 0.001 compared with non-treated.
그림 Figure 3. Effect of LVs on the cell cytotoxicity. (A) HaCaT, (B) HDF. (-); non-treated, lysis; lysis buffer treated. LVs; LVs treated. The results are expressed as the mean ± SD (N = 3). ***p < 0.001 compared with non-treated.
그림 Figure 4. Effect of LVs on fibrillin (FBN1) gene expression. (A) mRNA expressions of FBN1 were measured by qRT-PCR, (B) RT-PCR f or FBN1 mRNA. ( -); non-treated, LVs; LVs t reated (0.001%). The results are expressed as the mean ± SD (N = 3). ***p < 0.001 compared with non-treated.
그림 Figure 5. Effect of LVs on fibronectin (FN1) and filaggrin (FLG) gene expression. (A) mRNA expressions of FN1 and FLG were measured by qRT-PCR, (B) RT-PCR for FBN1 and FLG mRNA. (-); non-treated, LVs; LVs treated (0.001%). The results are expressed as the mean ± SD (N = 3). ***p < 0.001 compared with non-treated.
그림 Figure 6. Effect of LVs on collagen type 1 (COL1A1) expression. (-); non-treated, LVs; LVs t reated ( 0.001%). The results a re e xpressed as the mean ± SD (N = 3). ***p < 0.001 compared with non-treated.
그림 Figure 7. Effect of LVs on cornified envelop (CE) formation. (-); non-treated, Ca²⁺; Ca²⁺ treated, LVs; LVs treated (0.001%). The results are expressed as the mean ± SD (N = 3). ***p < 0.001 compared with non-treated.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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