[논문]둥근성게(Mesocentrotus nudus)를 이용한 과불화화합물의 생태독성평가

최훈; 이주욱; 이승민; 전형주; 허승; 황운기

doi:10.23005/ksmls.2021.6.2.80

[국내논문] 둥근성게(Mesocentrotus nudus)를 이용한 과불화화합물의 생태독성평가
Ecotoxicity Evaluation of PFCs using Marine Invertebrate, Sea Urchin (Mesocentrotus nudus) 원문보기

한국해양생명과학회지 = Journal of marine life science, v.6 no.2, 2021년, pp.80 - 87

최훈 (국립수산과학원 서해수산연구소 자원환경과) , 이주욱 (국립수산과학원 서해수산연구소 자원환경과) , 이승민 (국립수산과학원 서해수산연구소 자원환경과) , 전형주 (국립수산과학원 서해수산연구소 자원환경과) , 허승 (국립수산과학원 서해수산연구소 자원환경과) , 황운기 (국립수산과학원 어장환경과)

초록
AI-Helper

본 연구에서는 과불화합물 PFOA와 PFOS potassium salt가 Mesocentrotus nudus의 10 min-수정률과 48 h-정상유생발생률에 미치는 독성영향을 반수영향농도, 무영향농도, 최소영향농도 등의 독성값 계산을 통해 확인하였다. PFOA와 PFOS potassium salt에 대한 10 min-수정률의 EC₅₀ 값은 각각 1346.43 mg/l와 536.18 mg/l로 나타났으며, 48 h-정상유생발생률의 EC₅₀ 값은 각각 42.67 mg/l와 17.81 mg/l로 나타났다. 최근 연구에 의하면, 환경 내의 PFOA와 PFOS의 농도는 지속적으로 감소하였으며, 성게류에게 급성독성을 나타낼 정도는 아닌 것으로 나타났다. 하지만 생물체내에서는 여전히 높은 농도로 관측되고 있다. 결국, PFOA와 PFOS는 생물체의 생애전주기에 걸쳐 체내 축적이 가능하기 때문에, 연안환경에 서식하는 해양생물을 이용한 생애전주기적 만성독성 연구가 필요할 것이다.

Abstract ▼ AI-Helper

In this study, the toxic effects of PFOA and PFOS potassium salt on Mesocentrotus nudus using 10 min-fertilization rate and 48 h-normal embryogenesis were confirmed through the calculation of toxicity values such as Non-observed effective concentration, Low-observed effective concentration, and 50% of effective concentration. The case of 10 min-fertilization rate and 48 h-normal embryogenesis showed the concentration-dependent reduction pattern when exposed to PFOA and PFOS potassium salt, in tested concentration, respectively. The EC50 values of 10 min-fertilization rates for PFOA and PFOS potassium salt were 1346.43 mg/l and 536.18 mg/l, respectively, and the EC50 values of 48 h-normal embryogenesis were 42.67 mg/l and 17.81 mg/l, respectively. Both toxicity test methods showed high toxicity sensitivity to PFOS potassium salt. Recent studies have shown that the concentration of PFOA and PFOS in the marine environment has continuously decreased, and it is not enough to show acute toxicity to sea urchin. However, PFOA and PFOS have a very long half-life and can accumulate throughout the life of marine life, so it is still observed at a high concentration in shellfish. Therefore, a study on chronic toxicity through the whole-life cycle of marine organisms in coastal environments should be needed.

주제어

표/그림 (6)

표 Table 1. Information of the PFCs used in this study
그림 Fig. 1. Method for obtaining sperm (A) and egg (B) of Mesocentrotus nudus. Morphological method for visually measuring 10 min-fertilization rate and 48 h-normal embryogenesis. For 10 minfertilization rate, the presence or absence (C, D) of the fertilized membrane and for the normal embryogenesis, normal 4-arm development of the Pluteus larvae (E, F) were used morphologically classified.
그림 Fig. 2. Changes on 10 min-fertilization rate of Mesocentrotus nudus exposed to PFOA and PFOSK. Mean values represent the average concentrations (n = 4), and the vertical bar denotes ± 95% confidence interval (p < 0.05). Values with different superscripts are determined by Tukey's multiple range test (p < 0.05).
그림 Fig. 3. Changes on 48 h-normal embryogenesis rate of Mesocentrotus nudus exposed to PFOA and PFOSK. Mean values represent the average concentrations (n = 4), and the vertical bar denotes ± 95% confidence interval (p < 0.05). Values with different superscripts are determined by Tukey's multiple range test (p < 0.05).
표 Table 2. Information of experimental design used in this study
표 Table 3. Toxicity values, non-observed effect concentration (NOEC), Low-observed effective concentration (LOEC), and xx% effective concentration (EC_xx) for various endpoint of Sea-urchin species exposed to PFOA and PFOS

참고문헌 (52)

Ahrens L. 2011. Polyfluoroalkyl compounds in the aquatic environment: a reviewof their occurrence and fate. J Environ Monit 13: 20-31.

상세보기
Benford D, de Boer J, Carere A, di Domenico A, Johansson N, Schrenk D, Schoeters G, de Voogt P, Dellatte E. 2008. Opinion of the scientific panel on contaminants in the food chain on perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and their salts. EFSA J 653: 1-131.
Buck RC, Franklin J, Berger U, Conder JM, Cousins IT, de Voogt P, Jensen AA, Kannan K, Mabury SA, van Leeuwen SPJ. 2011. Perfluoroalkyl and polyfluoroalkyl substances in the environment: terminology, classification, and origins. Integr Environ Assess Manag 7: 513-541.

상세보기
Cai M, Yang H, Xie Z, Zhao Z, Wang F, Lu Z, Strum R, Ebinghaus R. 2012. Per-and polyfluoroalkyl substances in snow, lake, surface runoff water and coastal seawater in Fildes Peninsula, King George Island, Antarctica. J Hazard Mater 209: 335-342.

상세보기
Calafat AM, Wong LY, Kuklenyik Z, Reidy JA, Needham LL. 2007. Polyfluoroalkyl Chemicals in the U.S. Population: Data from the National Health and Nutrition Examination Survey (NHANES) 2003-2004 and Comparisons with NHANES 1999-2000. Environ Health Perspect 115: 1596-1602.

상세보기
Choi BI, Na SH, Son JH, Shin DS, Ryu BT, Chung SY. 2016. Biodegradation test of the alternatives of perfluorooctanesulfonate (PFOS) and PFOS salts. J Environ Health Sci 42: 112-117.
Choi H, Lee JW, Park YH, Lee SM, Choi YS, Heo S, Hwang UK. 2020a. Toxic effects of phenanthrene on fertilization and normal embryogenesis rates of Mesocentrotus nudus and Hemicentrotus pulcherrimus. Korean J Environ Biol 38: 333-342.
Choi H, Lee JW, Park YH, Lee SM, Choi YS, Heo S, Hwang UK. 2020b. Toxic effect of Zinc Undecylenate on the embryogenesis of Sea urchins Hemicentrotus pulcherrimus and Mesocentrotus nudus. J Mar Life Sci 5: 43-50.
Choi H, Park YH, Lee JW, Kwon KY, Hwang UK, 2020c. Toxic effects of new anti-fouling agents (diuron and irgarol) on the embryogenesis and developmental delay of sea urchin, Hemicentrotus pulcherrimus. Korean J Environ Biol 38: 518-527.
Dinglasan MJ, Ye Y, Edwards EA, Mabury SA. 2004. Fluorotelomer alcohol biodegradation yields poly- and perfluorinated acids. Environ Sci Technol 38: 2857-2864.

상세보기
Domingo JL. 2012. Health risks of dietary exposure to perfluorinated compounds. Environ Int 40: 187-195.

상세보기
Ellis DA, Mabury SA, Martin JW, Muir DCG. 2001. Thermolysis of fluoropolymers as a potential source of halogenated organic acids in the environment. Nature 412: 321-324.

상세보기
Giesy JP, Kannan K. 2001. Global distribution of perfluorooctane sulfonate in wildlife. Environ Sci Technol 35: 1339-1342.

상세보기
Giesy JP, Naile JE, Khim JS, Jones PD, Newsted JL. 2010. Aquatic toxicology of perfluorinated chemicals. Rev Environ Contam Toxicol 1-52.
Gilliland FD, Mandel JS. 1996. Serum perfluorooctanoic acid and hepatic enzymes, lipoproteins, and cholesterol: a study of occupationally exposed men. Am J Ind Med 29: 560-568.

상세보기
Hayman NT, Rosen G, Colvin MA, Conder J, Arblaster JA. 2021. Aquatic toxicity evaluations of PFOS and PFOA for five standard marine endpoints. Chemosphere 273: 129699.

상세보기
Hekster F, Laane R, De Voogt P. 2003. Environmental and toxicity effects of perfluoroalkylated substances. Rev Environ Contam Toxicol 179: 99-121.

상세보기
Higgins CP, Luthy RG. 2006. Sorption of perfluorinated surfactants on sediment. Environ Sci Technol 40: 7251-7256.
Hwang UK, Lee JW, Park YH, Heo S, Choi H. 2020. Toxic effects of antifouling agents (diuron and irgarol) on fertilization and normal embryogenesis rates in the sea urchin (Mesocentrotus nudus). Korean J Environ Biol 38: 207-215.
Kim HM. 2008. Environmental Risk Assessment of Perfluorinated Compounds (II). National Institute of Environmental.
Kim KY, Kim H, Choi S, Oh JE. 2020. Screening and validation of a pretreatment method for analyzing emerging fluorinated compounds in industrial wastewater samples. J Environ Anal Heal Toxicol 23: 90-100.
Krrman A, Harada KH, Inoue K, Takasuga T, Ohi E, Koizumi A. 2009. Relationship between dietary exposure and serum perfluorochemical (PFCS) levels-A case study. Environ Int 35: 712-717.
Kudo N, Kawashima Y. 2003. Toxicity and toxicokinetics of perfluorooctanoic acid in humans and animals. J Toxicol Sci 28: 49-57.

상세보기
Kwon BG, Lim CS, Lim HJ, Na SH, Kwon J, Chung SY. 2015. Monitoring of perfluorinated compounds (PFCs) in the Yeongsan river water system. J Korean Soc Environ Eng 37: 303-311.
Lau C, Anitole K, Hodes C, Lai D, Pfahles-Hutchens A, Seed J. 2007. Perfluoroalkyl acids: a review of monitoring and toxicological findings. Toxicol Sci 99: 366-394.

상세보기
Lee JW, Lee HK, Lim JE, Moon HB. 2020. Legacy and emerging per-and polyfluoroalkyl substances (PFASs) in the coastal environment of Korea: occurrence, spatial distribution, and bioaccumulation potential. Chemosphere 251: 126633.

상세보기
Lindstrom AB, Strynar MJ, Libelo EL. 2011. Polyfluorinated compounds: past, present and future. Environ Sci Technol 45: 7954-7961.

상세보기
Liu C, Yu K, Shi X, Wang J, Lam PKS, Wu RSS, Zhou B. 2007. Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Aquat Toxical 82: 135-143.
Liu Y, Shao M, Kuster WC, Goldan PD, Li X, Lu S, de Gouw JA. 2008. Source identification of reactive hydrocarbons and oxygenated VOCs in the summertime in Beijing. Environ Sci Technol 43: 75-81.
Logeshwaran P, Sivaram AK, Surapaneni A, Kannan K, Naidu R, Megharaj M. 2021. Exposure to perfluorooctanesulfonate (PFOS) but not perflurorooctanoic acid (PFOA) at ppb concentration induces chronic toxicity in Daphnia carinata. Sci Total Environ 769: 144577.

상세보기
Manzo S, Buono S, Cremisini C. 2006. Toxic effects of Irgarol and Diuron on sea urchin Paracentrotus lividus early development, fertilization, and offspring quality. Arch Environ Contam Toxicol 51: 61-68.

상세보기
Martin JW, Muir DCG, Moody CA, Ellis DA, Kwan WC, Solomon KR, Mabury SA. 2002. Collection of airborne fluorinated organics and analysis by Gas chromatography/chemical Ionization Mass spectrometry. Anal Chem 74: 584-590.

상세보기
Mhadhbi L, Rial D, Perez S, Beiras R. 2012. Ecological risk assessment of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) in marine environment using Isochrysis galbana, Paracentrotus lividus, Siriella armata and Psetta maxima. J Environ Monit 14: 1375-1382.
Ostertag SK, Chan HM, Moisey J, Dabeka R, Tittlemier SA. 2009. Historic dietary exposure to perfluorooctane sulfonate, perfluorinated carboxylates, and fluorotelomer unsaturated carboxylates from the consumption of store-bought and restaurant foods for the Canadian population. J Agr Food Chem 57: 8534-8544.
Paik BC, Kim TR, Kam SK. 2014. Distribution of Perfluorinated compounds (PFCs) in Sumjin river and southern coastal area of Korea. J of the Korean Society of Urban Environment 14: 87-99.
Paik BC, Kam SK. 2015. Annual detection characteristics of Perfluorinated compounds (PFCs) in the southern coastal water of south Korea. J of the Korean Society of Urban Environment 15: 75-83.
Paik BC, Kam SK. 2017. Detection characteristics of Perfluorinated compounds (PFCs) in the southern coastal sediment of south Korea and annual concentration in sea water. J of the Korean Society of Urban Environment 17: 231-241.
Parsons JR, Saez M, Dolfing J, de Voogt P. 2008. Biodegradation of perfluorinated compounds. Rev Environ Contam Toxicol 196: 53-71.

상세보기
Peden-Adams MM, EuDaly JG, Dabra S, EuDaly A, Heesemann L, Smythe J, Keil DE. 2007. Suppression of humoral immunity following exposure to the perfluoinated insecticide sulfluramid. J Toxicol Environ Health A 70: 1130-1141.
Peden-Adams MM, Keller JM, EuDaly JG, Berger J, Gilkeson GS, Keil DE. 2008. Suppression of humoral immunity in mice following exposure to perfluorooctane sulfonate. Toxicol Sci 104: 144-154.

상세보기
Prevedouros K, Cousins IT, Buck RC, Korzeniowski SH. 2006. Source, fate and transport of perfluorocarboxylates. Environ Sci Technol 40: 32-44.
Qazi MR, Xia Z, Bogdanska J, Chang SC, Ehresman DJ, Butenhoff JL, Nelson BD, DePierre JW, Abedi-Valugerdi M. 2009. The atrophy and changes in the cellular compositions of the thymus and spleen observed in mice subjected to short-term exposure to perfluorooctane sulfonate are high-dose phenomena mediated in part by peroxisome proliferatoractivated receptor-alpha (PPAR α). Toxicol 260: 68-76.
Shin MY, Im JK, Kho YL, Choi KS, Zoh KD. 2009. Quantitative determination of PFOA and PFOS in the effluent of sewage treatment plants and in Han river. Korean J Environ Health Sci 35: 334-342.
Shoeib M, Harner T, Vlahos P. 2006. Perfluorinated chemicals in the arctic atmosphere. Environ Sci Technol 40: 7577-7583.

상세보기
Son B, Lee L, Yang M, Park S, Pyo H, Lee W, Park J. 2017. Distribution and risk assessment of Perfluorinated compounds (PFCs) in major drinking water treatment plants, Korea. J Korean Soc Water and Wastewater 31: 491-499.
Stock NL, Lau FK, Ellis DA, Martin JW, Muir DC, Mabury SA. 2004. Polyfluorinated telomer alcohols and sulfonamides in the North American troposphere. Environ Sci technol 38: 991-996.

상세보기
Suja F, Pramanik BK, Zain SM. 2009. Contamination, bioaccumulation and toxic effects of perfluorinated chemicals (PFCs) in water environment: A review paper. Water Sci Technol 60: 1533-1544.
UNEP. 2009. Listing of perfluorooctane sulfonic acid, its salts and perfluorooctane sulfonyl fluoride in Annex B of the Stockholm Convention (UNEP/POPS/COP.4/SC-4/17). The Fourth Meeting of the Conference of the Parties to the Stockholm Convention on Persistent Organic Pollutants, Geneva, Switzerland.
UNEP. 2019. Listing of perfluorooctanoic acid (PFOA), its salts and PFOA-related compounds (UNEP/POPS/COP.9/SC-9/12). The Ninth Meeting of the Conference of the Parties to the Stockholm Convention on Persistent Organic Pollutants, Geneva, Switzerland.
Van Asselt ED, Rietra RPJJ, Romkens PFAM, van Der Fels-Klerx HJ. 2011. Perfluorooctane sulphonate (PFOS) throughout the food production chain. Food chem 128: 1-6.

상세보기
Yeo MK, Hwang EH, Jeong GH. 2012. Distribution characteristics of perfluorinated compounds in major river water and sediment. Anal Sci Technol 25: 313-323.
Yoon S, Lee BM, Heo W, Lee IS, Choi MK. 2018. Distribution of Perfluorinated compounds (PFCs) in seawater coastal areas in Korea. Symposium and conference of Environmental Health and Toxicology 271.

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