[논문]저수온기 참돔(Pagrus major) EP사료 내 동·식물성단백질 혼합물의 어분 대체

임종호; 김민기; 임현운; 이봉주; 이승형; 허상우; 김강웅; 이경준

doi:10.5657/kfas.2021.0350

저수온기 참돔(Pagrus major) EP사료 내 동·식물성단백질 혼합물의 어분 대체
Fish Meal Replacement with a Mixture of Plant and Animal Protein Sources in Extruded Pellet (EP) Diet for Red Seabream Pagrus major at Low Water Temperature 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.54 no.3, 2021년, pp.350 - 357

임종호 (제주대학교 해양생명과학과) , 김민기 (제주대학교 해양생명과학과) , 임현운 (제주대학교 해양생명과학과) , 이봉주 (국립수산과학원 사료연구센터) , 이승형 (국립수산과학원 사료연구센터) , 허상우 (국립수산과학원 사료연구센터) , 김강웅 (국립수산과학원 사료연구센터) , 이경준 (제주대학교 해양과학연구소)

Abstract ▼ AI-Helper

This study aimed to evaluate how fish meal (FM) replacement in diets with a mixture of animal and plant protein sources affect growth performance, feed utilization, hematological parameters and innate immunity of red seabream Pagrus major. A control FM diet was formulated to contain 65% FM (Con). Two other diets were prepared replacing FM in the control diet with a mixture of protein sources (wheat gluten, soy-protein concentrate, tankage meal, and poultry by-product meal) by 30 and 40% (FM30 and FM40, respectively). Total 300 red seabream (body weight, 77.6±0.3g) were distributed to 12 tanks (300 L) in 4 replicates per diet. The fish were fed the diets to apparent satiation for 19 weeks. After the feeding trial, no significant differences could be observed in growth performance, feed utilization, hematological parameters, innate immunity, and survivals among all the dietary treatments. This long-term feeding trial at low water temperature (13.8-17.5℃) indicates that a proper mixture ratio of wheat gluten, soy protein concentrate, tankage meal, and poultry by-product meal can replace FM up to 40% in red seabream diets.

주제어

표/그림 (5)

표 Table 1. Dietary formulation and proximate composition of the three experimental diets for red seabream Pagrus major (%, dry matter). The fish meal in diets was replaced with animal-plant protein mixture by 30 and 40% (Con, FM30 and FM40)
표 Table 2. Essential and non-essential amino acid composition of the experimental diets for red seabream Pagrus major (% of protein)
표 Table 3. Fatty acid composition of the experimental diets for red seabream Pagrus major (% of lipid)
표 Table 4. Growth performance, feed utilization and whole body proximate composition of red seabream Pagrus major fed experi-mental diets for 19 weeks (% of wet basis). The fsh meal in diets was replaced with animal-plant protein mixture by 30 and 40% (Con, FM30 and FM40)
표 Table 5. Hematological parameters and non-specific immune responses of red seabream Pagrus major (initial body weight, 77.6±0.3g) fed the experimental diets for 19 weeks. The fish meal in diets was replaced with animal-plant protein mixture by 30 and 40% (Con, FM30 and FM40)

AI 본문요약
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문제 정의

이번 연구에서는 어분의 대체원료로써 동∙식물성 단백질 혼합물(가금 부산 물분, 수지박, 밀글루텐, SPC)의 이용가능성을 평가하고자 참돔을 대상으로 저수온기에 장기간의 사육실험을 하였다. 특히, 산업현장에서의 적용가능성을 높이고자 extruded pellet (EP) 사료로 실험사료를 제작하여 연구를 수행하였다.
이번 연구에서는 어분의 대체원료로써 동∙식물성 단백질 혼합물(가금 부산 물분, 수지박, 밀글루텐, SPC)의 이용가능성을 평가하고자 참돔을 대상으로 저수온기에 장기간의 사육실험을 하였다. 특히, 산업현장에서의 적용가능성을 높이고자 extruded pellet (EP) 사료로 실험사료를 제작하여 연구를 수행하였다.

제안 방법

실험사료 조성표는 Table 1에 나타내었다. 대조사료(control, Con) 내 어분(sardine, anchoby, 1:1)의 함량은 65%가 되도록 설정하였고, 2종의 저어분 사료(FM30, FM40)는 밀글루텐, 대두 농축 단백, 수지박 및 가금부산물분으로 대조사료 내 어분을 각각 30, 40% 대체하도록 조성하였다. 실험사료는 국립수산과학원 사료연구센터에서 압출성형기(ATX-2; Fesco, Pohang, Korea)로 EP 형태의 부상사료로 제작하여 사용하였다.
사육실험 중 실험 어의 무게는 3주마다 측정하였으며 스트레스를 최소화하기 위해 측정 전 24시간 동안 절식하였다. 사육실험 종료 후에는 성장률(weight gain, WG), 일간성장률(specific growth rate, SGR), 사료섭취량(feed intake, FI), 사료계수(feed conversion ratio, FCR), 단백질전환효율(protein efficiency ratio, PER), 생존율 (survival)을 계산하였다.
3 g)은 총 12개의 원형 polypropylene 수조(300 L)에 수조 당 25마리씩, 실험 구당 4반복으로 무작위 배치되었다. 실험사료는 1일 2회(09:00, 17:30) 만복 공급하였다. 사육수는 저수온기 자연해수(13.
예비사육 후, 참돔(77.6±0.3 g)은 총 12개의 원형 polypropylene 수조(300 L)에 수조 당 25마리씩, 실험 구당 4반복으로 무작위 배치되었다

대상 데이터

실험사료의 아미노산과 지방산 조성은 Table 2와 Table 3에 각각 나타내었다. 사육실험에 사용된 참돔은 전남 고흥군 두원면에 위치한 정석 수산에서 구입하였다. 실험어는 운송 후 실험환경 적응을 위해 2 주간 순치하였다.
대조사료(control, Con) 내 어분(sardine, anchoby, 1:1)의 함량은 65%가 되도록 설정하였고, 2종의 저어분 사료(FM30, FM40)는 밀글루텐, 대두 농축 단백, 수지박 및 가금부산물분으로 대조사료 내 어분을 각각 30, 40% 대체하도록 조성하였다. 실험사료는 국립수산과학원 사료연구센터에서 압출성형기(ATX-2; Fesco, Pohang, Korea)로 EP 형태의 부상사료로 제작하여 사용하였다. 사육실험은 제주대학교 동물생명윤리위원회의 윤리규정(2020-0035) 을 준수하며 총 19주간의 장기간 진행되었다.

성능/효과

하지만 이번 연구에서는 동·식물성 단백질원료의 적절한 혼합물을 사용함으로써 결핍되기 쉬운 영양소를 서로 보완해 주었기 때문에 어분을 40%까지 대체하여도 참돔의 성장, 사료효율, 생존율, 건강도, 비특이적 면역력에 문제가 없었던 것으로 판단된다

후속연구

이번 연구의 저수온기(13.8-17.5℃) 외에 적수온, 고수온기에서의 추가연구를 통해 참돔 사료에서 동·식물성 단백질원료 혼합물의 최대 이용율을 조사할 필요가 있을 것으로 사료된다.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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