[논문]CRISPR/Cas 진단의 원리와 현황

박지웅; 강봉근; 신화희; 신준근

doi:10.9718/jber.2021.42.3.125

CRISPR/Cas 진단의 원리와 현황
The Principle and Trends of CRISPR/Cas Diagnosis 원문보기

Journal of biomedical engineering research : the official journal of the Korean Society of Medical & Biological Engineering, v.42 no.3, 2021년, pp.125 - 142

박지웅 (대구경북첨단의료산업진흥재단, 첨단의료기기개발지원센터, 진단의료기기팀) , 강봉근 (대구경북첨단의료산업진흥재단, 첨단의료기기개발지원센터, 진단의료기기팀) , 신화희 (대구경북첨단의료산업진흥재단, 첨단의료기기개발지원센터, 진단의료기기팀) , 신준근 (대구경북첨단의료산업진흥재단, 첨단의료기기개발지원센터, 진단의료기기팀)

Abstract ▼ AI-Helper

The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.

주제어

표/그림 (11)

그림 그림 1. 이중 가닥 DNA를 인식하는 리보핵단백질 Fig. 1. Schematic of Ribonucleoprotein during the recognition of dsDNA
그림 그림 2. CRISPR/Cas 시스템을 활용한 측방 유동 분석법(ACS Nano 2020;14:2497-2508에서 발췌 및 수정) Fig. 2. Scheme of representative lateral flow assay based on CRISPR/Cas system(Wang et al. ACS Nano 2020;14:2497-2508)
표 표 1. Cas 단백질의 특성 Table 1. Properties of Cas proteins
그림 그림 3. CRISPR/Cas 색도분석센서의 개념도(Pardee et al. Cell 2016;165:1255-1266에서 발췌 및 수정) Fig. 3. Schematic representation of CRISPR Cleavage sensor(Pardee et al. Cell 2016;165:1255-1266)
그림 그림 4. CRISPR/Cas 금 나노 입자 센서 개략도(Yuan et al. Anal. Chem. 2020;92:4029-4037 발췌 및 수정) Fig. 4. Schematic presentation of colorimetric detection based on gold nanoparticle and CRISPR/Cas system(Yuan et al. Anal. Chem. 2020;92:4029-4037)
그림 그림 5. CRISPR/Cas gFET 센서의 개략도(Hajian et al. Nat. Biomed. Eng. 2019;3:427-437 발췌 및 수정) Fig. 5. CRISPR/Cas gFET sensor(Hajian et al. Nat. Biomed. Eng. 2019;3:427-437)
그림 그림 6. 전기화학 CRISPR 센서의 개략도(Xu et al. Biosens. Bioelectron. 2020;155:112100 발췌 및 수정) Fig. 6. Schematic representation of electrochemical CRISPR sensor(Xu et al. Biosens. Bioelectron. 2020;155:112100)
그림 그림 7. CRISPR/Cas 형광검출법에 활용되는 부수적 절단의 개략도(East-Seletsky et al. Nature 2016;538:270-273 발췌 및 수정) Fig. 7. Schematic representation of trans cleavage of fluorescent RNA probe(East-Seletsky et al. Nature 2016; 538:270-273)
그림 그림 8. Luciferase를 융합한 Cas9 쌍을 활용한 발광 센서의 개략도(Zhang et al. ACS Synth. Biol. 2017;6:211-216 발췌 및 수정) Fig. 8. Schematic representation of in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase(Zhang et al. ACS Synth. Biol. 2017;6:211-216)
그림 그림 9. 백금입자를 활용한 미세유체 칩 상의 CRISPR/Cas 센서의 작동 원리(Shao et al. Anal. Chem. 2019;91:12384-12391. 발췌 및 수정)Fig. 9. Working principle of the platinum nanoreporter-based CRISPR-Cas12a detection system on the microfluidic chip(Shao et al. Anal. Chem. 2019;91:12384-12391)
표 표 2. CRISPR/Cas를 활용한 형광 검출법의 비교 Table 2. Comparison of CRISPR/Cas based fluorescent biosensing systems

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Wu H, Qian C, Wu C, et al. End-Point Dual Specific Detection of Nucleic Acids Using CRISPR/Cas12a Based Portable Biosensor. Biosens. Bioelectron. 2020;157:112153.

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Peng L, Zhou J, Liu G, et al. CRISPR-Cas12a Based Aptasensor for Sensitive and Selective ATP Detection. Sensors Actuators, B Chem. 2020;320:128164.

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Li H, Li M, Yang Y, et al. Aptamer-Linked CRISPR/Cas12a-Based Immunoassay. Anal. Chem. 2021;93:3209-3216.

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Xiong Y, Zhang J, Yang Z, et al. Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets. J. Am. Chem. Soc. 2020;142:207-213.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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