[논문]저항성 운동과 유산소 운동 훈련의 병행이 노화쥐 골격근 유형별 혈관신생 관련 단백질 발현에 미치는 영향

여효성

doi:10.12925/jkocs.2021.38.3.750

저항성 운동과 유산소 운동 훈련의 병행이 노화쥐 골격근 유형별 혈관신생 관련 단백질 발현에 미치는 영향
Effect of a combination of resistance and aerobic exercise training on angiogenesis-related protein expression in different type of skeletal muscle of aged rats 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.38 no.3, 2021년, pp.750 - 761

여효성 (분당서울대학교병원 재활의학과)

초록
AI-Helper

이 연구는 노화된 흰쥐를 대상으로 규칙적인 저항성 운동에 유산소 운동을 병행하는 훈련을 실시하여 골격근에서 나타나는 혈관신생 관련 단백질 발현의 반응을 관찰하기 위해 수행되었다. 연구의 목적을 위해 자연적으로 노화된 SD계열 흰쥐(20-24개월령, N=18)를 사용하여 통제(CON, n=6), 저항성 운동(RE, n=6), 저항성+유산소 운동(RE+AE, n=6) 집단으로 구분하였다. 저항성 운동 집단은 실험동물용 사다리를 이용하여 매회 3세트×4회의 운동을 실시하였고 저항성 운동+유산소 운동 집단은 매회 2세트×3회의 사다리 오르기와 추가적인 30분간의 트레드밀 달리기를 수행하였다. 총 8주간의 운동 훈련 종료 후 가자미 근과 장지신근을 적출하여 분석에 사용하였다. 골격근에서 혈관신생 관련 단백질들(HIF-1α, VEGF, FLK-1, Ang-1, Ang-2)의 발현 수준을 분석하기 위해 western blot을 실시하였다. 연구결과, 저항성+유산소 운동 집단에서 가자미근(type I 근육)의 HIF-1α, VEGF, FLK-1, Ang-1, Ang-2 단백질 발현이 통제집단에 비해 높았으며 저항성 운동만 수행할 경우 HIF-1α, VEGF, Ang-1, Ang-2 단백질 발현이 통제집단에 비해 높았다. 또한 가자미근에서 저항성+유산소 운동훈련 집단의 Ang-2 to Ang-1 ratio가 저항성 운동 집단에 비해 높아 운동훈련 유형별 차이를 보였다. 한편, 장지신근(type II 근육)에서 HIF-1α는 저항성 운동 훈련에 의해서만 증가된 반면 VEGF와 FLK-1 단백질 발현은 두 훈련 유형 모두에서 증가되었고 운동 훈련 유형별 차이는 관찰되지 않았다. 또한 장지신근의 angiopoieitin 단백질들의 발현은 운동 훈련에 의한 차이가 없었다. 그러므로 노화에서 규칙적인 운동 훈련은 운동 유형에 관계없이 골격근 혈관신생 반응을 유도하며, 특히 저항성 운동에 유산소 운동의 병행은 type I 근조직 유형에서 혈관신생에 대한 추가적인 긍정적 효과를 가질 수 있다.

Abstract ▼ AI-Helper

This study was performed to observe the responses of angiogenesis-related protein expression in skeletal muscle of aged rats by regular resistance exercise training with aerobic exercise. For the purpose of the study, naturally aged SD rats (20-24 months, N=18) were used and divided into control (CON, n=6), resistance exercise (RE, n=6), and resistance + aerobic exercise (RE + AE, n=6) groups. RE group performed 3 sets × 4 exercises each session using a ladder for laboratory animals, and RE +AE group performed 2 sets × 3 times of ladder climbing and additional treadmill running (30 min) each session. After 8 weeks of exercise training, soleus muscle and extensor digitorum longus muscle (EDL) were extracted and used for analysis. Western blot was performed to analyze the expression levels of angiogenesis-related proteins (HIF-1α, VEGF, FLK-1, Ang-1, Ang-2) in skeletal muscle. As a result of the study, the expression of HIF-1α, VEGF, FLK-1, Ang-1, and Ang-2 proteins in soleus muscle (type I muscle) was higher in RE +AE than in CON group, and HIF-1α, VEGF, Ang-1, Ang-2 protein expression of RE group was higher than that of CON group. Furthermore, Ang-2 to Ang-1 ratio of RE + AE group was higher than that of RE group, showing differences by exercise type. In EDL muscle (type II muscle), HIF-1α was increased only by RE group, whereas VEGF and FLK-1 protein expressions were increased in both training types, and no difference was observed between the types of exercise training. In addition, there was no difference in angiopoieitin protein expressions in EDL muscle by exercise training. Therefore, in aging, regular exercise training induces skeletal muscle angiogenic response regardless of exercise type, and in particular, the combination of aerobic exercise with resistance exercise may have an additional positive effect on angiogenesis in type I muscle.

주제어

표/그림 (2)

그림 Fig. 1. Differences of protein expression related with angiogenesis in type I muscle in different exercise training mode. Mean±SD. a: significane from CON (p<.05). b: significane from RE (p<.05).
그림 Fig. 2. Differences of protein expression related with angiogenesis in type II muscle in different exercise training mode. Mean±SD. a: significane from CON (p<.05). b: significane from RE (p<.05).

참고문헌 (43)

L. Hosseini, M. S. Vafaee, R. Badalzadeh, "Melatonin and nicotinamide mononucleotide attenuate myocardial ischemia/reperfusion injury via modulation of mitochondrial function and hemodynamic parameters in aged rats", Journal of cardiovascular pharmacology and therapeutics, Vol.25, No.3, pp. 240-250, (2020).

상세보기
R. Roubenoff, V. A. Hughes, "Sarcopenia: current concepts", The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, Vol.55, No.12, pp. M716-M724, (2000).

상세보기
M. J. Rennie, "Anabolic resistance: the effects of aging, sexual dimorphism, and immobilization on human muscle protein turnover", Applied Physiology, Nutrition, and Metabolism, Vol.34, No.3, pp. 377-381, (2009).

상세보기
C. P. Lambert, W. J. Evans, "Effects of aging and resistance exercise on determinants of muscle strength", Journal of the American Aging Association, Vol.25, No.2, pp. 73-78, (2002).

상세보기
S. Joanisse, J. P. Nederveen, T. Snijders, B. R. McKay, G. Parise, "Skeletal muscle regeneration, repair and remodelling in aging: the importance of muscle stem cells and vascularization", Gerontology, Vol.63, No.1, pp. 91-100, (2017).

상세보기
B. J. Behnke, M. W. Ramsey, J. N. Stabley, J. M. Dominguez 2nd, R. T. Davis 3rd, D. J. McCullough, J. M. Muller Delp, M. D. Delp, "Effects of aging and exercise training on skeletal muscle blood flow and resistance artery morphology", Journal of applied physiology, Vol.113, No.11, pp. 1699-1708, (2012).

상세보기
S. Egginton, I. Badr, J. Williams, D. Hauton, G. C. Baan, R. T. Jaspers, "Physiological angiogenesis is a graded, not threshold, response", The Journal of physiology, Vol.589, No.1, pp. 195-206, (2011).

상세보기
K. Tang, E. C. Breen, H. P. Gerber, N. M. Ferrara, P. D. Wagner, "Capillary regression in vascular endothelial growth factor-deficient skeletal muscle", Physiological genomics, Vol.18, No.1, 63-69, (2004).

상세보기
N. A. Ryan, K. A. Zwetsloot, L. M. Westerkamp, R. C. Hickner, W. E. Pofahl, T. P. Gavin, "Lower skeletal muscle capillarization and VEGF expression in aged vs. young men", Journal of applied physiology, Vol.100, No.1, pp. 178-185, (2006).

상세보기
A. N. Croley, K. A. Zwetsloot, L. M. Westerkamp, N. A. Ryan, A. M. Pendergast, R. C. Hickner, W. E. Pofahl, T. P. Gavin, T. P, "Lower capillarization, VEGF protein, and VEGF mRNA response to acute exercise in the vastus lateralis muscle of aged vs. young women", Journal of applied physiology, Vol.99, No.5, pp. 1872-1879, (2005).

상세보기
H. S. Yeo, J. Y. Lim, N. Y. Ahn, "Effects of Aging on Angiogenic and Muscle Growth-Related Factors in Naturally Aged Rat Skeletal Muscles", Annals of geriatric medicine and research, Vol.24, No.4, pp. 305, (2020).

상세보기
M. Ardakanizade, "The effects of mid-and long-term endurance exercise on heart angiogenesis and oxidative stress", Iranian journal of basic medical sciences, Vol.21, No.8, pp. 800, (2018).

상세보기
N. Charifi, F. Kadi, L. Feasson, F. Costes, A. Geyssant, C. Denis, "Enhancement of microvessel tortuosity in the vastus lateralis muscle of old men in response to endurance training", The Journal of physiology, Vol.554, No.2, pp. 559-569, (2004).

상세보기
X. W. Cheng, M. Kuzuya, W. Kim, H. Song, L. Hu, A. Inoue, K. Nakamura, Q. Di, T. Sasaki, M. Tsuzuki, G. Shi, K. Okumura, T. Murohara, "Exercise training stimulates ischemia-induced neovascularization via phosphatidylinositol 3-kinase/Akt-dependent hypoxia-induced factor-1 α reactivation in mice of advanced age", Circulation, Vol.122, No.7, pp. 707, (2010).

상세보기
T. M. Holloway, T. Snijders, V. L. LJC, L. B. Verdijk, "Temporal response of angiogenesis and hypertrophy to resistance training in young men", Medicine and science in sports and exercise, Vol.50, No.1, pp. 36-45, (2018).

상세보기
N. Ahn, K. Kim, "Effects of Aerobic and Resistance Exercise on Myokines in High Fat Diet-Induced Middle-Aged Obese Rats", International journal of environmental research and public health, Vol.17, No.8, pp. 2685, (2020).

상세보기
H. T. Roh, S. Y. Cho, W. Y. So, "A Cross-Sectional Study Evaluating the Effects of Resistance Exercise on Inflammation and Neurotrophic Factors in Elderly Women with Obesity", Journal of clinical medicine, Vol.9, No.3, pp. 842. (2020).

상세보기
P. G. Lloyd, B. M. Prior, H. T. Yang, R. L. Terjung, "Angiogenic growth factor expression in rat skeletal muscle in response to exercise training", American Journal of Physiology-Heart and Circulatory Physiology, Vol.284, No.5, pp. H1668-H1678, (2003).

상세보기
J. L. Williams, A. Weichert, A. Zakrzewicz, L. Da Silva-Azevedo, A. R. Pries, O. Baum, S. Egginton, "Differential gene and protein expression in abluminal sprouting and intraluminal splitting forms of angiogenesis", Clinical science, Vol.110, No.5, pp. 587-595, (2006).

상세보기
T. R. Lundberg, R. Fernandez-Gonzalo, T. Gustafsson, P. A. Tesch, "Aerobic exercise does not compromise muscle hypertrophy response to short-term resistance training", Journal of applied physiology, Vol.114, No.1, pp. 81-89, (2013).

상세보기
A. G. Philippe, G. Py, F. B. Favier, A. M. Sanchez, A. Bonnieu, T. Busso, R. Candau, "Modeling the responses to resistance training in an animal experiment study", BioMed research international, Vol. 2015. (2015).

상세보기
E. Koltai, Z. Zhao, Z. Lacza, A. Cselenyak, G. Vacz, C. Nyakas, I. Boldogh, N. Ichinoseki-Sekine, Z. Radak, "Combined exercise and insulin-like growth factor-1 supplementation induces neurogenesis in old rats, but do not attenuate age-associated DNA damage", Rejuvenation research, Vol.14, No.6, pp. 585-596, (2011).

상세보기
D. C. Lee, R. R. Pate, C. J. Lavie, X. Sui, T. S. Church, S. N. Blair, "Leisure-time running reduces all-cause and cardiovascular mortality risk", Journal of the American College of Cardiology, Vol.64, No.5, pp. 472-481, (2014).

상세보기
S. C. Moore, A. V. Patel, C. E. Matthews, A. B. de Gonzalez, Y. Park, H. A. Katki, M. S. Linet, E. Weiderpass, K. Visvanathan, K. J. Helzlsouer, M. Thun, S. M. Gapstur, P. Hartge, I. M. Lee, "Leisure time physical activity of moderate to vigorous intensity and mortality: a large pooled cohort analysis", PLoS Med, Vol.9, No.11, pp. e1001335. (2012).

상세보기
K. A. Larkin, R. G. MacNeil, M. Dirain, B. Sandesara, T. M. Manini, T. W. Buford, "Blood flow restriction enhances post-resistance exercise angiogenic gene expression", Medicine and science in sports and exercise, Vol.44, No.11, pp. 2077, (2012).

상세보기
L. N. Forti, E. Van Roie, R. Njemini, W. Coudyzer, I. Beyer, C. Delecluse, I. Bautmans, "Dose-and gender-specific effects of resistance training on circulating levels of brain derived neurotrophic factor (BDNF) in community-dwelling older adults", Experimental gerontology, Vol.70, pp. 144-149, (2015).

상세보기
D. R. Slivka, M. W. Heesch, C. L. Dumke, J. S. Cuddy, W. S. Hailes, B. C. Ruby, "Human skeletal muscle mRNA response to a single hypoxic exercise bout", Wilderness & environmental medicine, Vol.25, No.4, pp. 462-465, (2014).

상세보기
M. J. Drummond, S. Fujita, A. Takashi, H. C. Dreyer, E. Volpi, B. B. Rasmussen, "Human muscle gene expression following resistance exercise and blood flow restriction", Medicine and science in sports and exercise, Vol.40, No.4, pp. 691. (2008).

상세보기
P. Rodriguez-Miguelez, E. Lima-Cabello, S. Martinez-Florez, M. Almar, M. J. Cuevas, J. Gonzalez-Gallego, "Hypoxia-inducible factor-1 modulates the expression of vascular endothelial growth factor and endothelial nitric oxide synthase induced by eccentric exercise", Journal of applied physiology, Vol.118, No.8, pp. 1075-1083, (2015).

상세보기
A. Ahmadi, D. Sheikholeslami-Vatani, S. Ghaeeni, M. Baazm, "The effects of different training modalities on monocarboxylate transporters MCT1 and MCT4, hypoxia inducible factor-1α (HIF-1α), and PGC-1α gene expression in rat skeletal muscles", Molecular Biology Reports, Vol.48, No.3, pp. 2153-2161, (2021).

상세보기
M. E. Lindholm, H. Rundqvist, "Skeletal muscle hypoxia-inducible factor-1 and exercise", Experimental physiology, Vol.101, No.1, pp. 28-32, (2016).

상세보기
O. Hudlicka, M. D. Brown, "Adaptation of skeletal muscle microvasculature to increased or decreased blood flow: role of shear stress, nitric oxide and vascular endothelial growth factor", Journal of vascular research, Vol.46, No.5, pp. 504-512, (2009).

상세보기
L. Jensen, J. Bangsbo, Y. Hellsten, "Effect of high intensity training on capillarization and presence of angiogenic factors in human skeletal muscle", The Journal of physiology, Vol. 557, No.2, pp. 571-582, (2004).

상세보기
I. M. Olfert, R. A. Howlett, K. Tang, N. D. Dalton, Y. Gu, K. L. Peterson, P. P. Wagner, E. C. Breen, "Muscle-specific VEGF deficiency greatly reduces exercise endurance in mice", The Journal of physiology, Vol.15, No.587(Pt 8), pp. 1755-1767, (2009).
T. P. Gavin, R. M. Kraus, J. A. Carrithers, J. P. Garry, R. C. Hickner, "Aging and the skeletal muscle angiogenic response to exercise in women", Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, Vol.70, No.10, pp. 1189-1197, (2015).

상세보기
M. Ratajczak, D. Skrypnik, P. Bogdanski, E. Madry, J. Walkowiak, M. Szulinska, J. Meciaszek, M. Kregielska-Narozna, J. Karolkiewicz, Effects of Endurance and "Endurance-Strength Training on Endothelial Function in Women with Obesity: A Randomized Trial", International journal of environmental research and public health, Vol.16, No.21, pp. 4291, (2019).

상세보기
R. Fernandez-Gonzalo, T. R. Lundberg, P. A. Tesch, "Acute molecular responses in untrained and trained muscle subjected to aerobic and resistance exercise training versus resistance training alone", Acta Physiologica, Vol.209, No.4, pp. 283-294, (2013).

상세보기
B. Hoier, K. Olsen, D. J. Hanskov, M. Jorgensen, L. R. Norup, Y. Hellsten, "Early time course of change in angiogenic proteins in human skeletal muscle and vascular cells with endurance training", Scandinavian Journal of Medicine & Science in Sports, Vol.30, No.7, pp. 1117-1131, (2020).

상세보기
S. D. Mason, H. Rundqvist, I. Papandreou, R. Duh, W. J. McNulty, R. A. Howlett, I. M. Olfert, C. J. Sundberg, N. C. Denko, L. Poellinger, R. S. Johnson, "HIF-1α in endurance training: suppression of oxidative metabolism", American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol.293, No.5, pp. R2059-R2069, (2007).

상세보기
M. Bellafiore, G. Battaglia, A. Bianco, A. Palma, "Expression pattern of angiogenic factors in healthy heart in response to physical exercise intensity", Frontiers in physiology, Vol.10, pp. 238, (2019).

상세보기
C. Suri, J. McClain, G. Thurston, D. M. McDonald, H. Zhou, E. H. Oldmixon, T. N. Sato, G. D. Yancopoulos, "Increased vascularization in mice overexpressing angiopoietin-1", Science, Vol.282, No.5388, pp. 468-471, (1998).

상세보기
A. I. Nykanen, K. Pajusola, R. Krebs, M. A. Keranen, O. Raisky, P. K. Koskinen, K. Alitalo, K. B. Lemstrom, "Common protective and diverse smooth muscle cell effects of AAV-mediated angiopoietin-1 and-2 expression in rat cardiac allograft vasculopathy", Circulation research, Vol.98, No.11, pp. 1373-1380, (2006).

상세보기
T. P. Gavin, J. L. Drew, C. J. Kubik, W. E. Pofahl, R. C. Hickner, "Acute resistance exercise increases skeletal muscle angiogenic growth factor expression", Acta physiologica, Vol.191, No.2, pp. 139-146, (2007).

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format