[논문]HaCaT cell에서 황련 추출물(Coptis chinensis)의 피부보습과 항염증 효과

김은희; 문영란; 장영아

doi:10.12925/jkocs.2021.38.3.870

HaCaT cell에서 황련 추출물(Coptis chinensis)의 피부보습과 항염증 효과
Skin Moisturizing Properties and Anti-Inflammatory effects of extracts from Coptis chinensis in HaCaT cells 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.38 no.3, 2021년, pp.870 - 882

김은희 (구월 화장품연구소) , 문영란 (비비솔루션(주)) , 장영아 (경성대학교 스마트헬스케어융복합연구센터)

초록
AI-Helper

황련은 한의학에서 진정, 소염, 항균 및 해열 등의 여러 질병의 치료에 이용되어져 왔다. 본 연구에서는 UVB와 TNF-α/IFN-γ로 유도된 HaCaT 세포에서 피부장벽과 염증관련 인자에 대한 황련 열수 추출물(CCW)의 효과를 조사하고, 보습 및 항염증 소재로서의 가능성을 알아보고자 하였다. 각 질형성세포인 HaCaT 세포에서 CCW의 보습 및 항염 효과를 확인하기 위해 MTT assay를 통한 시료의 독성 평가를 실시하였다. 이를 바탕으로, HA(Hyaluronic acid) 생성량과 filaggrin의 protein 및 mRNA 발현을 측정하였다. TNF-α/IFN-γ로 자극된 HaCaT 세포에서 CCW는 농도 의존적으로 HA의 생성량을 증가 시켰고, filaggrin의 protein 및 mRNA 발현 측정 결과 CCW의 농도 증가에 따라 발현율도 증가하였다. UVB로 유도된 HaCaT 세포에서 CCW는 ROS의 생성을 감소 시켰고, 양성대조군(positive control)인 EGCG( (-)-epigallocatechin-3-gallate)와 유의한 결과를 보여 주었다. 또한, CCW는 염증성 사이토카인 TNF-α, IL-1β, IL-6, IL-8의 발현을 억제 시켰고, TNF-α/IFN-γ로 자극된 HaCaT 세포에서 피부 염증에 따른 주요 인자인 COX-2의 protein 및 mRNA 발현이 농도 의존적으로 감소되는 것을 확인할 수 있었다. 이러한 결과는, 황련 열수 추출물의 보습 및 항염증 효과를 가지는 화장품 소재로서의 가치를 제안할 수 있다.

Abstract ▼ AI-Helper

Coptis chinensis has been used in the treatment of various diseases such as soothing, anti-inflammation, antimicrobial and antipyretic in oriental traditional medicine. In this study, we investigated the effect of hot water extract of Coptis chinensis(CCW) on skin barrier and inflammation-related factors in UVB and TNF-α/IFN-γ-induced HaCaT cells and evaluated its potential as a moisturizing and anti-inflammatory material. Based on result, the amount of HA (Hyaluronic acid) production and protein and mRNA expression of filaggrin were measured. In TNF-α/IFN-γ-induced HaCaT cells, CCW increased the amount of HA production in a concentration-dependent manner. In the measurement of protein and mRNA expression of filaggrin, the expression rate increased as the concentration of CCW increased. In UVB-induced HaCaT cells, CCW decreased the production of ROS and showed significant results with EGCG ((-)-epigallocatechin-3-gallate), a positive control. In addition, CCW inhibited the expression of inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-8 in TNF-α/IFN-γ-induced HaCaT cells. It was confirmed that the protein and mRNA expression of COX-2, a major factor in skin inflammation, was decreased in a concentration-dependent manner. These results suggest that hot water extract from Coptis chinensis can be used as a cosmetic material having a moisturizing and anti-inflammatory effect.

주제어

표/그림 (7)

표 Table 1. The Sequence of Primers used for PCR
그림 Fig. 1. Cell viability of CCW on treated HaCaT cells. HaCaT cells were treated with various concentrations (12.5 – 200 μg/mL) of CCW. Cell viability was measured using the MTT assay. CCW : Coptis chinensis water extract. The results are presented as mean ± standard deviation of triplicate date. The results were expressed as the average of triplicate. *p<0.05.
그림 Fig. 2. Effect of CCW in the expression of hyaluronic acid in TNF-α/IFN-γ-induced HaCaT cells. Cells were treated for 24 h with CCW (12.5, 25, 50 μg/mL) during TNF-α/IFN- γ-indueced. Nor : Non treated TNF-α/IFN-γ. Cont : TNF-α/IFN-γ induced group. CCW : Coptis chinensis water extract. EGCG : (-)-epigallocatechin-3-gallate (positive control). The results are presented as mean ± standard deviation of triplicate date. The results were expressed as the average of triplicate. *p<0.05.
그림 Fig. 3. Expressions of filaggrin evaluated by protein level(A) and mRNA level(B) in TNF-α/IFN- γ-induced HaCaT cells after CCW treatment. (A)The expression levels of filaggrin in the protein was measured after treatment of TNF-α/IFN-γ for 24 h and the indicated concentration of CCW. (B)The expression levels of filaggrin in the mRNA was measured after treatment of TNF-α/IFN-γ for 4 h and the indicated concentration of CCW. Nor : Non treated TNF-α/IFN-γ. Cont : TNF-α/IFN-γ induced group. CCW : Coptis chinensis water extract. EGCG : (-)-epigallocatechin-3-gallate (positive control). The results are presented as mean ± standard deviation of triplicate date. The results were expressed as the average of triplicate. *p<0.05.
그림 Fig. 4. Effect of CCW in the expression of ROS in the UVB-induced HaCaT cells. Cells were treated for 24 h with CCW (12.5, 25, 50 μg/mL) during UVB (20 mJ/cm2) activation. Nor : Non treated UVB. Cont : UVB induced group. CCW : Coptis chinensis water extract. EGCG : (-)-epigallocatechin-3-gallate (positive control). The results are presented as mean ± standard deviation of triplicate date. The results were expressed as the average of triplicate. *p<0.05
그림 Fig. 5. Effects of CCW on productions of pro-inflammatory cytokines in TNF-α/IFN-γ-induced HaCaT cells. The expression levels of TNF-α (A), IL-1β (B), IL-6 (C), IL-8 (D) in the protein was measured after treatment of TNF-α/IFN-γ for 18 h and the indicated concentration of CCW. Nor : Non treated TNF-α/IFN-γ. Cont : TNF-α/IFN-γ induced group. CCW : Coptis chinensis water extract. EGCG : (-)-epigallocatechin-3- gallate (positive control). The results are presented as mean ± standard deviation of triplicate date. The results were expressed as the average of triplicate. *p<0.05
그림 Fig. 6. Expressions of COX-2 evaluated by protein level(A) and mRNA level(B) in TNF-α/IFN- γ-induced HaCaT cells after CCW treatment. (A)The expression levels of COX-2 in the protein was measured after treatment of TNF-α/IFN-γ for 24 h and the indicated concentration of CCW. (B)The expression levels of COX-2 in the mRNA was measured after treatment of TNF-α/IFN-γ for 4 h and the indicated concentration of CCW. Nor : Non treated TNF-α/IFN-γ. Cont : TNF-α/IFN-γ induced group. CCW : Coptis chinensis water extract. EGCG : (-)-epigallocatechin-3-gallate (positive control). The results are presented as mean ± standard deviation of triplicate date. The results were expressed as the average of triplicate. *p<0.05.

참고문헌 (37)

H. J. Kim, J. U. Shin and K. H. Lee. "Atopic dermatitis and skin barrier dysfunction.", Allergy Asthma & Respiratory Disease, Vol.1, No.1 pp. 20-28, (2013).

상세보기
O. K. Jacobi. "About the mechanism of moisture regulation in the horny layer of the skin", Proceedings of the Scientific Section of the Toilet Goods Association, Vol.31, No.5 pp. 22-24, (1959).
C. S. Park, "Skin barrier and moisturizer", The Korean Society for Skin Barrier Research, Vol.9, No.1 pp. 11-17, (2007).
L. Hruza and A. P. Pentland, "Mechanisms of UV-induced inflammation", Journal of Invastigative Dermatology, Vol.100, No.1 pp. 35-41, (1993).

상세보기
H. J. Kim, "Atopic dermatitis and skin barrier dysfuntion", Allergy Asthma & Respiratory Disease, Vol.1, No.1 pp. 20-28, (2013).

상세보기
P. M. Steinert, J. S. Cantieri, D. C. Teller, J. D. Lonsdale-Eccles and B. A. Dale. "Characterization of a class of cationic proteins that specifically interact with intermediate filaments", Proceedings of the National Academy of Sciences of the United State of America, Vol.78, No.40 pp. 97-101, (1981).
C. R. Harding, A. Watkinson, A. V. Rawlings and I. R. Scott, "Dry skin, moisturization and corneodesmolysis." International Journal of Cosmetic Science, Vol.22, No.1 pp. 21-52, (2000)

상세보기
C. F. Nathan, "Secretory products of macrophages", The Journal of Clinical Invastigation, Vol.79, No.2 pp. 319-326, (1987).

상세보기
C. Maziere, M. A. Conte, J. Degonville, D. Ali, and J. C. Maziere, "Cellular enrichment with polyunsaturated fatty acids induces an oxidative stressand activates the transcription factors AP-1 and NF-kappaB.", Biochemical and Biophysical Research Communications, Vol.265, No.1 pp. 116-122, (1999).

상세보기
K. T. Kim, Y. H. Kim, J. G. Kim, C. S. Han, S. H. Park, and B. Y. Lee, "Preparation of oligo hyaluronic acid by hydrolysis and its application as a cosmetic ingredient.", Journal of the Society of Cosmetic Scientists of Korea, Vol.33, No.3 pp. 189-196, (2007).
J. Necas, L. Bartosikova, P. Braunder, and J. Kolar, "Hyaluronic acid (hyaluronan): a review", Veterinarni Medicina, Vol.53, No.8 pp. 397-411, (2008).

상세보기
P. J. Lee, H. T. Kim, K. S. Yoon, H. C. Park, and H. Y. Ha, "The effecct of Astragalus membranceus methanol extract on hyaluronic acid procudtion in HaCaT cells.", The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology, Vol.26, No.1 pp.75-81, (2013).

원문보기 상세보기
H. J. Song, H. J. Mu, and S. H. Lee, "Effect of Ferulic acid isolated from Cnidium officinaale on the synthesis of hyaluronic acid.", Journal of the Society of Cosmetic Scientists of Korea, Vol.39, No.4 pp. 281-288, (2013).

원문보기 상세보기
R. Briante, F. Febbraio and R. Nucci, "Antioxidant properties of low molecular weight phenols present in the mediterranean diet.", Journal of Agricultural and Food Chemistry, Vol.51, No.24, pp. 6975-6981, (2003).

상세보기
V. Morrisette-Thomas, A. A. Cohen, T. Fulop, E. Riesco, V. Legault, Q. Li, E. Milot, F. Dusseault-Belanger and L. Ferrucci, "Inflamm-aging does not simply reflect increases in pro-inflammatory markers.", Mechanisms of Ageing and Development, Vol.Jul, No.139 pp. 49-57, (2014).
M. Helenius, L. Makelainen and A. Salminen, "Attenuation of NF-kappa B signaling response to UV B light during cellular senescence.", Experimental Cell Research, Vol.248, No.1 pp. 194-202, (1999).

상세보기
D. Walker and H. Jacobe, "Phototherapy in the age of biologics.", Seminars in Cutaneous Medicine and Surgery, Vol.30, No.4 pp. 190-198, (2011).

상세보기
T. E. Strandberg and R. S. Tilvis, "C-reactive protein, cardiovascular risk factors, and mortality in a prospective study in the elderly.", Arteriosclerosis, Thrombosis, and Vascular Biology, Vol.20, No.4 pp. 1057-1060, (2000).

상세보기
H. Bruunsgaard, "Effects of tumor necrosis factor-alpha and interleukin-6 in elderly populations.", European Cytokine Network, Vol.13, No.4 pp. 389-391, (2002).

상세보기
C. D. Funk, "Prostaglandins and leukotrienes: advances in eicosanoid biology.", Science, Vol.294, No.5548 pp. 1871-1875. (2001).

상세보기
Y. J. Ji, J. W. Lee and I. S. Lee, "Antimicrobial effect of medicinal plants against methicllin-resistant Staphylococcus aureus(MRSA).", Korean Society of Life Science, Vol.17, No.3 pp. 412-419, (2007).
B. S. Kim, H. S. Jang, C. S. Choi, J. S. Kim, G. S. Kwon, I. S. Kwun, K. H. Son and H. Y. Sohn, "Antifungal activity of Zanthaxylum schinifolium against Fusarium graminearum, a Barley Powdery Mildew Fungus.", Journal of Life Science, Vol.18, No.7 pp. 974-979, (2008).

원문보기 상세보기
D. U. Lee, Y. J. Kang, M. K. Park, Y. S. Lee, H. G. Seo, T. S. Kim, C. H. Kim and K. C. Chang, "Effects of 13-alkylsubstituted berberine alkaloids on the expression of COX-II, TNF-α, iNOS, and IL-12 production in LPS-stimulated macrophages.", Journal of Life Science, Vol.73, No.11 pp. 1401-1412, (2003).

상세보기
E. K. Kim, K. B. Kwon, M. J. Han, M. Y. Song, J. H. Lee, N. Lv , S. O. Ka, S. R. Yeom, Y. D. Kwon, D. G. Ryu, K. S. Kim, J. W. Park, R. Park and B. H. Park, "Coptidis rhizoma extract protects against cytokine-induced death of pancreatic beta-cells through suppression of NF-κB activation.", Experimental & Molecular Medicine. Vol.39, No.2 pp. 149-159, (2007).

상세보기
R. Enk, R. Ehehalt, J. E. Graham, A. Bierhaus, A. Remppis and H. J. Greten, "Differential effect of Rhizoma coptidis and its main alkaloid compound berberine on TNF-α induced NF-κB translocation in human keratinocytes.", Journal of Ethnopharmacology, Vol.109, No.1 pp. 170-175, (2007).

상세보기
T. Mosmann, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", Journal of Immunological Methods, Vol.65, No.1-2 pp. 55-63, (1983).

상세보기
B. Dube, H. J. Luke, M. Aumailley, and P. Prehm, "Hyaluronan reduces migration and proliferation in CHO cells.", Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol.1538, No.2-3 pp. 283-289, (2001).

상세보기
H. J. Song, M. H. Jin, and S. H. Lee, "Effect of ferulic acid isolated from Cnidium Officinale on the synthesis of hyaluronic acid.", Journal of the Society of Cosmetic Scientists of Korea, Vol.39, No.4 pp. 281-288, (2013).

원문보기 상세보기
P. Dahiya and R. Kamal, "Hyaluronic Acid: a boon in periodontal therapy.", North American Journal of Medical Sciences, Vol.5, No.5 pp. 309-315, (2013).

상세보기
B. Debasis, "Green tea: Antioxidant Power to Fight Disease." pp.25-48, Keats Publishing, (1999)
G. M. O'Regan and A. D. Irvine, "The role of filaggrin in the atopic diathesis.", Clinical & Experimental Allergy, Vol.40, No.7 pp. 965-972, (2010).

상세보기
H. J. Kim, J. U. Shin and K. H. Lee, "Atopic dermatitis and skin barrier dysfunction.", Allergy, Asthma & Respiratory Disease, Vol.1, No.1 pp. 20-28, (2013).

상세보기
A. M. Cameron, A. Castoldi, D. E. Sanin, L. J. Flachsmann, C. S. Field, D. J. Puleston, R. L. Kyle, A. E. Patterson, F. Hassler, J. M. Buescher, B. Kelly and E. L. Pearce, "Inflammatory macrophage dependence on NAD+ salvage is a consequence of reactive oxygen speciesmediated DNA damage.", Nature Immunology, Vol.20, No.4 pp. 420-432, (2019).

상세보기
L. Kong, J. Liu, J. Wang, Q. Luo, H. Zhang, B. Liu, F. Xu, Q. Pang, Y. Liu and J. Dong, "Icariin inhibits TNF-α/IFN-γ induced inflammatory response via inhibition of th substance P and p38-MAPK signaling pathway in human keratinocytes." International Immunopharmacology, Vol.29, No.2 pp. 401-407, (2015).

상세보기
J. H. Yang, Y. H. Hwang, M. J. Gu, W. K. Cho and J. Y. Ma, "Ethanol extracts of Sanguisorba officinalis L. suppress TNF-α/IFN-γ-induced pro-inflammatory chemokine production in HaCaT cells.", Phytomedicine, Vol.22, No.14 pp. 1262- 1268, (2015).

상세보기
Y. Y. Sung, Y. S. Kim and H. K. Kim, "Illicium verum extract inhibits TNF-α- and IFN-γ-induced expression of chemokines and cytokines in human keratinocytes." Journal of Ethnopharmacology, Vol.144, No.31 pp. 182-189, (2012).

상세보기
C. D. Funk, "Prostaglandins and leukotrienes: advances in Eicosanoid Biology.", Journal of Science, Vol.294, No.5548 pp. 1871-1875, (2001).

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증