[논문]1, 2-Octanediol과 1, 2-Octanediol Galactoside의 항균력 및 세포독성 비교연구

김준섭; 진홍종; 정경환

doi:10.12925/jkocs.2021.38.3.629

1, 2-Octanediol과 1, 2-Octanediol Galactoside의 항균력 및 세포독성 비교연구
Comparative Study of Antimicrobial and Cytotoxic Effects of 1, 2-Octanediol and 1, 2-Octanediol Galactoside 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.38 no.3, 2021년, pp.629 - 637

김준섭 (한국교통대학교 생명공학전공) , 진홍종 ((주)케이피티 연구소) , 정경환 (한국교통대학교 생명공학전공)

초록
AI-Helper

안전성이 강화된 화장품용 방부제 소재를 개발하기 위하여, 대장균 β-gal을 이용하여 OD로부터 합성된 OD-gal의 성능을 기존의 OD와 비교하는 연구를 수행하였다. 먼저, mass spectrometry 분석을 통하여 OD-gal의 sodium adduct ion (m/z=331.1731)과 protonated ion (m/z=309.1926)으로 OD-gal 합성을 확인하였다. 새롭게 합성된 OD-gal과 OD의 항균력 비교를 위하여 E. coli, S. aureus, C. albicans, A. niger에 대한 MIC 값을 측정하였다. 그 결과, OD-gal과 OD의 MIC 값 사이에 그렇게 큰 차이를 관찰할 수 없었다. 또한, OD-gal과 OD의 세포독성을 비교하기 위하여, HaCaT 세포에 OD 또는 OD-gal을 처리한 후, 세포 생존율을 EZ-Cytox assay를 이용하여 정량 하였다. 1.5% OD의 경우에는 24 시간에서 세포생존율이 대조군과 비교하여 64%, 48 시간에서는 42%로 나타났고, 1.5% OD-gal을 처리한 세포의 세포생존율은 24 시간에서 유의적인 변화가 없었지만, 48 시간에서는 85%로 나타났다. 결국 OD-gal은 OD와 비교해서 약 40% 이상의 세포독성 감소효과가 있는 것으로 확인되었다. 이러한 결과로부터 OD에 한 분자의 galactose를 결합시킴으로 인하여 항균력은 어느 정도 유지하면서 피부 세포에 대한 독성이 감소된 OD-gal의 특성을 확인할 수 있었다. 앞으로 후속 연구를 진행하여 보다 안전한 화장품용 방부제 소재로서 OD-gal의 실용화 기반을 구축할 예정이다.

Abstract ▼ AI-Helper

To develop a safer cosmetic preservative, we carried out a comparative study on characteristics of OD and OD-gal, where OD-gal was synthesized from OD using E. coli β-gal. OD-gal synthesis was confirmed by mass spectrometry analysis as sodium adduct ion (m/z=331.1731) and protonated ion (m/z=309.1926) of OD-gal. To compare the antimicrobial activities of OD and newly synthesized OD-gal, MIC values were investigated using E. coli, S. aureus, C. albicans, and A. niger. As a result, it was observed that there was no remarkable difference between MIC values of OD and OD-gal. In addition, to compare the cytotoxicity of OD-gal and OD, HaCaT cells were treated with OD or OD-gal, and then cell viability was quantified using EZ-Cytox assay. In the case of 1.5% OD, the cell viability was 64% at 24 h and 42% at 48 h compared to the control, and cell viability of 1.5% OD-gal-treated cells showed no significant change at 24 h and was 85% at 48 h. Consequently, the cytotoxicity of OD-gal-treated cells was reduced by more than 40% when compared with that of OD-treated cells. Thus, the newly synthesized OD-gal in this study is safer than the existing OD used as a cosmetic additive. In the future, OD-gal will be applicable as a substitute for OD as a less toxic preservative for the cosmetic industry.

주제어

표/그림 (5)

그림 Fig. 1. Enzymatic synthesis of OD-gal using β-gal.
그림 Fig. 2. TLC analysis for OD (lane 1), the reaction mixture (lane 2), and purified OD-gal (lane 3). The reaction mixture was collected at the elapsed time of 48 h. Arrow a and b indicate OD and OD-gal, respectively.
그림 Fig. 3. High-resolution ESI mass spectrometry of purified OD-gal. [M+Na]⁺ and [M+H]⁺ indicate sodium adduct and protonated ion of OD-gal, respectively. The preferred structures of OD-gal are shown in the mass spectrum.
그림 Fig. 4. MICs of OD and OD-gal. 1, 2, 3 and 4 on X-axis indicate MICs of OD (ref2), OD (ref18), OD (ref19) and OD (ref20), respectively. OD (ref2), OD (ref18), OD (ref19) and OD (ref20) mean MIC data from previously published studies [2,18,19,20]. 5 and 6 on X-axis indicate MICs of OD and OD-gal, respectively, in this study. All measurements in this study were performed three times (n=3) using the same sample, and the average and standard deviation were calculated.
그림 Fig. 5. Cytotoxic effects of OD and OD-gal in HaCaT cells. Cells were treated with the indicated dose of OD or OD-gal for 24 h (A) or 48 h (B) and then cell viability was measured by using EZ-CYTOX assay. The statistical analysis of the data was carried out by use of a t-test. **p < 0.01, ***p < 0.001 compared to controls. ###p < 0.001 was compared to 1.5% OD and 1.5% OD-gal treated group. All measurements were performed three times (n=3) using the same sample, and the average and standard deviation were calculated.

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