[논문]Taxus chinensis로부터 파클리탁셀 정제를 위한 개선된 아세톤-물 분별침전 공정 개발 및 그 동역학 및 열역학적 해석

강회종; 김진현

doi:10.9713/kcer.2021.59.3.379

Taxus chinensis로부터 파클리탁셀 정제를 위한 개선된 아세톤-물 분별침전 공정 개발 및 그 동역학 및 열역학적 해석
Development of An Improved Acetone-Water Fractional Precipitation Process for Purification of Paclitaxel from Taxus chinensis and Its Kinetic and Thermodynamic Analysis 원문보기

Korean chemical engineering research = 화학공학, v.59 no.3, 2021년, pp.379 - 392

초록
AI-Helper

본 연구에서는 초음파 캐비태이션 버블와 가스 버블를 이용한 파클리탁셀의 개선된 아세톤-물 분별침전 공정을 개발하였다. 전통적 방법에 비해 침전에 소요되는 시간이 20~25배 단축되었다. 또한 파클리탁셀의 침전물 크기는 3.5~5.5배 감소하였으며 파클리탁셀의 확산 계수는 3.5~6.7배 증가하였다. 초음파 캐비태이션 버블을 이용한 침전의 경우 초음파 파워는 증가할수록, 침전 온도는 감소할수록 침전 속도 상수는 증가하였다. 가스 버블을 이용한 침전의 경우 가스 유량은 증가할수록, 침전 온도는 감소할수록 침전 속도 상수는 증가하였다. 열역학적 해석을 통해 개선된 분별침전은 비자발적 발열 공정이었다.

Abstract ▼ AI-Helper

In this study, an improved acetone-water fractional precipitation process for paclitaxel using ultrasonic cavitation bubbles and gas bubbles was developed. Compared to the conventional method, the time required for precipitation has been reduced by 20~25 times. In addition, the particle size of paclitaxel decreased by 3.5~5.5 times and the diffusion coefficient of paclitaxel increased by 3.5~6.7 times. In the case of precipitation using ultrasonic cavitation bubbles, as the ultrasonic power increased and the temperature decreased, the precipitation rate constant increased. In the case of precipitation using gas bubbles, as the gas flow rate increased and the temperature decreased, the precipitation rate constant increased. Thermodynamic parameters revealed the exothermic, irreversible, and nonspontaneous nature of the improved fractional precipitation.

주제어

표/그림 (14)

$Fig. 1. Schematic diagram of conventional fractional precipitation (A), fractional precipitation with ultrasonic cavitation bubble (B), and fractional precipitation with gas bubble (C) for pre-purification of paclitaxel.$ 그림 Fig. 1. Schematic diagram of conventional fractional precipitation (A), fractional precipitation with ultrasonic cavitation bubble (B), and fractional precipitation with gas bubble (C) for pre-purification of paclitaxel.
$Fig. 2. Effect of precipitation time on the yield of paclitaxel obtained by conventional acetone-water fractional precipitation at 5 ºC. (A) Sample purity 20.4%; (B) Sample purity 63.6%.$ 그림 Fig. 2. Effect of precipitation time on the yield of paclitaxel obtained by conventional acetone-water fractional precipitation at 5 ºC. (A) Sample purity 20.4%; (B) Sample purity 63.6%.
$Fig. 3. Effect of ultrasonic power on the yield of paclitaxel obtained by ultrasound-assisted acetone-water fractional precipitation at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.$ 그림 Fig. 3. Effect of ultrasonic power on the yield of paclitaxel obtained by ultrasound-assisted acetone-water fractional precipitation at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.
$Fig. 4. Effect of gas flow rate on the yield of paclitaxel obtained by acetone-water fractional precipitation with gassing unit at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.$ 그림 Fig. 4. Effect of gas flow rate on the yield of paclitaxel obtained by acetone-water fractional precipitation with gassing unit at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.
$Fig. 5. Johnson-Mehl-Avrami-Kolmogorov (JMAK) plots at different sample purities for conventional acetone-water fractional precipitation at 5 ºC.$ 그림 Fig. 5. Johnson-Mehl-Avrami-Kolmogorov (JMAK) plots at different sample purities for conventional acetone-water fractional precipitation at 5 ºC.
$Table 1. Effect of ultrasonic cavitation bubbles and gas bubbles on mean particle size and diffusivity in acetone-water fractional precipitation$ 표 Table 1. Effect of ultrasonic cavitation bubbles and gas bubbles on mean particle size and diffusivity in acetone-water fractional precipitation
$Table 2. Kinetic parameters for the acetone-water fractional precipitation with ultrasonic cavitation bubble at different ultrasound powers$ 표 Table 2. Kinetic parameters for the acetone-water fractional precipitation with ultrasonic cavitation bubble at different ultrasound powers
$Fig. 6. Johnson-Mehl-Avrami-Kolmogorov (JMAK) plots for acetone-water fractional precipitation with ultrasonic cavitation bubble at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.$ 그림 Fig. 6. Johnson-Mehl-Avrami-Kolmogorov (JMAK) plots for acetone-water fractional precipitation with ultrasonic cavitation bubble at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.
$Fig. 7. Johnson-Mehl-Avrami-Kolmogorov (JMAK) plots for acetone-water fractional precipitation with gas bubble at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.$ 그림 Fig. 7. Johnson-Mehl-Avrami-Kolmogorov (JMAK) plots for acetone-water fractional precipitation with gas bubble at different sample purities and temperatures. (A) Sample purity 20.4% and 5 ºC; (B) Sample purity 63.6% and 5 ºC; (C) Sample purity 20.4% and 15 ºC; (D) Sample purity 63.6% and 15 ºC; (E) Sample purity 20.4% and 25 ºC; (F) Sample purity 63.6% and 25 ºC.
$Table 3. Kinetic parameters for the acetone-water fractional precipitation with gas bubble at different gas flow rates$ 표 Table 3. Kinetic parameters for the acetone-water fractional precipitation with gas bubble at different gas flow rates
$Table 4. Thermodynamic parameters for the acetone-water fractional precipitation with ultrasonic cavitation bubble at different ultrasound powers$ 표 Table 4. Thermodynamic parameters for the acetone-water fractional precipitation with ultrasonic cavitation bubble at different ultrasound powers
$Table 5. Thermodynamic parameters for the acetone-water fractional precipitation with gas bubble at different gas flow rates$ 표 Table 5. Thermodynamic parameters for the acetone-water fractional precipitation with gas bubble at different gas flow rates
$Table 6. Activation parameters for the acetone-water fractional precipitation with ultrasonic cavitation bubble at different ultrasound powers$ 표 Table 6. Activation parameters for the acetone-water fractional precipitation with ultrasonic cavitation bubble at different ultrasound powers
$Table 7. Activation parameters for the acetone-water fractional precipitation with gas bubble at different gas flow rates$ 표 Table 7. Activation parameters for the acetone-water fractional precipitation with gas bubble at different gas flow rates

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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