[논문]Apigenin Ameliorates Oxidative Stress-induced Neuronal Apoptosis in SH-SY5Y Cells

Kim, Yeo Jin; Cho, Eun Ju; Lee, Ah Young; Seo, Weon Taek

doi:10.48022/mbl.2009.09006

Apigenin Ameliorates Oxidative Stress-induced Neuronal Apoptosis in SH-SY5Y Cells 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.2, 2021년, pp.138 - 147

Kim, Yeo Jin (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) , Cho, Eun Ju (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) , Lee, Ah Young (Department of Food Science, Gyeongsang National University) , Seo, Weon Taek (Department of Food Science, Gyeongsang National University)

Abstract ▼ AI-Helper

The overproduction of reactive nitrogen species (RNS) and reactive oxygen species (ROS) causes oxidative damage to neuronal cells, leading to the progression of neurodegenerative diseases. In this study, we determined the nitric oxide radical (NO), hydroxyl radical (·OH), and superoxide anion radical (O2-) scavenging activities of apigenin. Our results showed that apigenin exhibited remarkable, concentration-dependent ·OH, O2-, and NO radical scavenging activities. Particularly, apigenin indicated the strongest ·OH radical scavenging activity with 93.38% in the concentration of 100 µM. Furthermore, we also investigated the protective effects of apigenin against hydrogen peroxide (H2O2)-induced oxidative stress in SH-SY5Y cells. The H2O2 treatment resulted in a significant decrease in cell viability, as well as an increase in lactate dehydrogenase (LDH) release and ROS production compared with the H2O2-nontreated SH-SY5Y cells. However, the cell viability significantly increased in the apigenin-treated group, as well as inhibited ROS generation and LDH release compared with the H2O2-induced control group. To elucidate the protective mechanisms of apigenin against oxidative stress in SH-SY5Y, we analyzed the apoptosis-related protein expression. The apigenin treatment resulted in the downregulated expression of apoptosis-related protein markers, such as cytochrome C, cleaved caspase-3, poly (ADP)-ribose polymerase (PARP), and B-cell lymphoma 2-associated X (Bax), as well as the upregulated expression of anti-apoptosis markers such as B-cell lymphoma 2 (Bcl-2). In this study, we report that apigenin exhibits a neuroprotective effect against oxidative stress in SH-SY5Y cells. These results suggest that apigenin may be considered as a potential agent for neurodegenerative disease prevention.

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표/그림 (5)

표 Table 1. ·OH, O₂ ^-, and NO radical scavenging activity of apigenin.
그림 Fig. 1. Effect of apigenin on cell viability in SH-SY5Y cells treated with H₂O₂. Values are mean ± SD. ^a-dMeans are significantly different (p < 0.05) by Duncan's multiple range test.
그림 Fig. 2. Effect of apigenin on ROS production in SH-SY5Y cells treated with H₂O₂. Time course of change in intensity of DCF fluorescence during 60 min (A). Intensity of ROS production at 60 min (B). Values are mean ± SD. ^a-dMeans are significantly different (p < 0.05) by Duncan's multiple range test.
그림 Fig. 3. Effect of apigenin on LDH release in SH-SY5Y cells treated with H₂O₂. Values are mean ± SD. ^a-dMeans are significantly different (p < 0.05) by Duncan's multiple range test.
그림 Fig. 4. Effects of apigenin on the levels of Bax/Bcl-2 (A) cytochrome C (B) cleaved caspase-3 (C) cleaved PARP/PARP (D) protein expression in SH-SY5Y cells treated with H₂O₂. Bax, B-cell lymphoma 2-associated X; Bcl-2, B-cell lymphoma 2; cytochrome C; cleaved caspase-3; PARP, poly (ADP)-ribose polymerase. Values are mean ± SD. ^a-eMeans are significantly different (p < 0.05) by Duncan’s multiple range test. β-actin was used as a loading control.

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