[논문]Galactose에 순치한 Saccharomyces cerevisiae를 이용하여 꼬시래기(Gracilaria verrucosa)로부터 바이오에탄올 생산

양지원; 박유림; 정귀택; 김성구

doi:10.48022/mbl.2009.09005

Galactose에 순치한 Saccharomyces cerevisiae를 이용하여 꼬시래기(Gracilaria verrucosa)로부터 바이오에탄올 생산
Bioethanol Production from Gracilaria verrucosa Using Saccharomyces cerevisiae with Adaptive Evolution 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.1, 2021년, pp.88 - 94

양지원 (부경대학교 생물공학과) , 박유림 (부경대학교 생물공학과) , 정귀택 (부경대학교 생물공학과) , 김성구 (부경대학교 생물공학과)

초록
AI-Helper

해조류 중 홍조류인 꼬시래기(G. verrucosa)로부터 효모를 이용한 발효를 위해 열산가수분해, 효소당화 및 에탄올 발효수율 향상을 검토하고, 기존의 혼합당의 흡수효율을 높이기 위해 고농도 당 순치를 수행하였다. 열산가수분해는 200 mM 황산(H₂SO₄)을 이용하여 10% (w/v)의 꼬시래기(G. verrucosa)의 슬러리, 130℃의 온도에서 60분 동안 열산가수분해를 수행하였다. 또한 wild type 효모와 고농도 galactose에 순치(adaptive evolution)된 효모를 이용한 발효를 실시한 결과, wild type 효모의 경우 발효 144시간에 8.5 g/l 에탄올 발효로 에탄올수율계수 Y_EtOH = 0.19와 galactose에 순치된 효모의 경우 21.5 g/l 에탄올 발효로 에탄올수율계수 Y_EtOH = 0.50을 나타내었다. 이러한 연구결과는 해양 바이오매스인 해조류로부터 바이오 연료의 효율적인 생산방법을 제공할 수 있을 것으로 판단된다.

Abstract ▼ AI-Helper

The seaweed, Gracilaria verrucosa (red seaweed) was fermented to produce bioethanol. Optimal thermal acid hydrolysis conditions were determined as 200 mM H2SO4 and 10% (w/v) seaweed slurry at 130℃ for 60 min yielding 47.5% of pretreatment efficiency (Ep). After the thermal acid hydrolysis, enzymatic saccharification was carried out with 16 U/ml Viscozyme L, Cellic CTec2 or mixture of Viscozyme L and Cellic CTec2 to G. verrucosa hydrolysates. Enzymatic saccharifications with Viscozyme, Cellic CTec2 or mixture of those yielded 7.3 g/l glucose with efficiency of saccharification, Es = 34.9%, 11.6 g/l glucose with Es = 64.4% and the mixture of those 9.6 g/l glucose with Es = 56.6%, respectively. Therefore, based on the Es value, Cellic CTec2 was selected for the optimal enzyme for enzymatic saccharification of G. verrucosa hydrolysate. The ethanol productions with non-adapted S. cerevisiae CEN-PK2 (wild type) and S. cerevisiae CEN-PK2 with adaptive evolution to galactose produced 8.5 g/l ethanol with YEtOH = 0.19 and 21.5 g/l ethanol with YEtOH = 0.50 at 144 h, respectively. From these results, the ethanol production by S. cerevisiae with adaptive evolution showed high concentration of ethanol production using G. verrucosa as a substrate.

주제어

표/그림 (7)

그림 Fig. 1. Effect of thermal acid hydrolysis and efficiency of pretreatments. Various acids (H₂SO₄, HNO₃ and HCl) were used with 10% (w/v) G. verrucosa slurry content, 200 mM at 121℃ for 60 min. Values labeled with different letters (A, B, C, D for glucose; a, b, c, d, e for galactose) differ significantly based on one-way ANOVA and Duncan's multiple range test (p < 0.05).
표 Table 1. Composition of Gracilaria verrucosa.
그림 Fig. 2. Effect of thermal acid hydrolysis and efficiency of pretreatments. (A) sulfuric acid (H₂SO₄) concentrations, (B) hydrochloric acid (HCl) concentrations on the efficiency of pretreatment carried out with 10% (w/v) slurry content, at 121℃ for 60 min. Values labeled with different letters (A, B, C, D for glucose; a, b, c, d, e for galactose) differ significantly based on one-way ANOVA and Duncan's multiple range test (p < 0.05).
그림 Fig. 3. Evaluation of thermal acid hydrolysis conditions by changing the treatment parameters. (A) reaction temperature, (B) thermal acid hydrolysis time and (C) slurry content. Values labeled with different letters (A, B, C, D for glucose; a, b, c, d, e for galactose) differ significantly based on one-way ANOVA and Duncan's multiple range test (p < 0.05).
그림 Fig. 4. Saccharification of single and mixed enzyme treatments on glucose release of G. verrucosa hydrolysate from 10% (w/v) slurry after thermal acid hydrolysis at pH 5.0, 50℃ for 72 h.
그림 Fig. 5. Effect of activated carbon treatment with the adsorption time of 2 min on HMF removal and monosaccharide concentration at 150 rpm, 50℃.
그림 Fig. 6. Bioethanol production from G. verrucosa using thermal acid hydrolysis, enzymatic saccharification and fermentation using (A) wild type S. cerevisiae CEN-PK2, (B) S. cerevisiae CEN-PK2 with adaptive evolution.

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