[논문]쌍별귀뚜라미(Gryllus bimaculatus)의 l(2)efl cDNA 클로닝과 발현분석

권기상; 이누리; 권오유

doi:10.5352/jls.2021.31.7.671

초록
AI-Helper

쌍별 귀뚜라미(Gryllus bimaculatus)에서 lethal (2) essential for life [l(2)efl]을 코드한 cDNA를 분리하여 GBl(2)efl이라 하였다. GBl(2)efl는 N-glycosylation 한곳과 phosphorylation site를 15곳 가진 189 aa로 구성되며6.2등전점과 21.19 kDa 분자량을 가진다. GBl(2)efl 단백질의 이차구조는 random coils (56.08%), alpha-helix (22.22%), extended strands (17.99%), beta turns (3.7%)로 이루어진다. GBl(2)efl 는 지금까지 보고된 l(2)efl들과는48-69%의 상동성을 보인다. GBl(2)efl은1일, 3일 starvation일때에 각각 dorsal longitudinal flight muscle과 Malpighian tubules에서 mRNA발현이 증가하였다. 한편, ER stress 조건에서는GBl(2)efl 발현은 fat body에서 증가하였다. 본 연구는 곤충의 생존에 기여하는 생리학적 메커니즘을 이해와 효과적인 해충 관리 통제를 수행할 수 있는 능력을 향상에 많은 힌트를 줄 수 있는 실마리를 제공할 수 있을 것이다.

Abstract ▼ AI-Helper

A cDNA encoding the protein lethal (2) essential for life [l(2)efl] was cloned from Gryllus bimaculatus and named GBl(2)efl. This protein is composed of 189 amino acids, including an N-glycosylation site and 15 phosphorylation sites. Its predicted molecular mass is 21.19 kDa, with a theoretical isoe...

A cDNA encoding the protein lethal (2) essential for life [l(2)efl] was cloned from Gryllus bimaculatus and named GBl(2)efl. This protein is composed of 189 amino acids, including an N-glycosylation site and 15 phosphorylation sites. Its predicted molecular mass is 21.19 kDa, with a theoretical isoelectric point of 6.2. The secondary structure of GBl(2)efl was predicted from the identification of random coils (56.08%), alpha helices (22.22%), extended strands (17.99%), and beta turns (3.7%) through sequence analyses. A homology analysis revealed that GBl(2)efl exhibited a high similarity with other species at the amino acid level, ranging from 52% to 69%. While GBl(2)efl mRNA expression was higher in the dorsal longitudinal flight muscle following a three-day starvation and in the Malpighian tubules following a one-day starvation, no changes in expression were detected in other tissues. Furthermore, tunicamycin-induced endoplasmic reticulum (ER) stress resulted in an approximately 1.8-fold higher expression in the fat body compared with the wild type.

Keyword

표/그림 (3)

그림 Fig. 1. The nucleotide and deduced amino acid sequences of GBl(2)efl. GBl(2)efl cDNA encodes a protein of 189 amino acids. The predicted amino acid sequence (single-letter abbreviation) is shown below the nucleotide sequence within the open reading frame. Fourteen phosphorylation sites are indicated by shading for the 8 serines (S), 5 threonines (T), and 2 tyrosines (Y), while an N-glycosylation site is circled (N). The amino acid sequence of the GBl(2)efl protein has been submitted to GenBank, and the accession number is MN205433.
그림 Fig. 2. Comparison of GBl(2)efl sequences. (A) Alignment of the GBl(2)efl with homologs from eight others species. Identical amino acid residues in this alignment are indicated by dark gray stars (*). Highly-conserved regions of amino acid residues are indicated by gray colons (:), and weakly-conserved regions of amino acid residues are indicated by light gray points (.). Deleted positions in the amino acid residues are indicated by dashes. (B) A molecular phylogenetic tree of GBl(2)efl. Phylogenetic tree based on homologs of GBl(2)efl from seven species constructed by the NCBI program. The length of the section indicates relative distances between the sequences. The species and gene accession numbers are as follows: Tribolium castaneum (XP_968760.1), Drosophila melanogaster (NP_523827.1), Aedes aegypti (XP_001663494.1), Bombyx mori (NP_001091767.1), Apis mellifera (XP_001120194.1), Cryptotermes secundus (XP_023705725.1), Blattella germanica (PSN45237.1.), Nilaparvata lugens (XP_022188126.1), and Gryllus bimaculatus (MN205433).
그림 Fig. 3. GBl(2)efl gene expression. (A) In the various tissues. (B) Under ER stress. Tunicamycin (5 μl) was injected intraperitoneally three times across 2 days. (C) Under starvation or refeeding conditions. S1–6, starvation for 1–6 days; S6+R1, a day of refeeding after 6 days of starvation; S6+R2; 2 days of refeeding after 6 days of starvation. DL, dorsal longitudinal flight muscle; DV, dorsal ventral flight muscle; DW, dorsal wing flight muscle; FB, fat body; FG, foregut; MG, midgut; HG, hindgut; MP, Malpighian tubules. Values are presented as mean ± SEM, n = 3, *p<0.05 **p<0.005 ***p<0.001. Statistical significance between multiple groups: one-way analysis of variance test. Graph Pad Prism 6 software (Graph Pad Software Inc.).

AI 본문요약
AI-Helper

성능/효과

The protein sequence of GBl(2)efl was compared with those of other known l(2)efl homologs in the GenBank database, which showed a 52% homology with that from D. melanogaster (NP_523827.1) and 69% with that from Cryptotermessecundus (XP_023705725.1) (Fig. 2A).
However, considering that the homology between GBl(2)efl and other identified l(2)efl’s were relatively high (52%-69%), the unique biological function of GBl(2)efl may be conserved across different species.
2A). GBl(2)efl exhibits an independent evolution pattern from the early stages of evolution with other l(2)efl homologs (Fig. 2B).

후속연구

Furthermore, sites for N-terminal acetylation, C-terminal mannosylation, and GalNAc O-glycosylation were not detected in GBl(2)efl. Information on these protein structures provide valuable insights into the unique functions of GBl(2)efl and should be explored in future studies. The secondary structure of GBl(2)efl was predicted from sequence analyses showing the following domains: random coils (56.

참고문헌 (17)

Ahn, M. Y., Han, J. W., Kim, S. J., Hwang, J. S. and Yun, E. Y. 2011. Thirteen-week oral dose toxicity study of G. bimaculatus in sprague-dawley rats. Toxicol. Res. 27, 231-240.
Ahn, M. Y., Hwang, J. S., Yun, E. Y., Kim, M. J. and Park, K. K. 2015. Anti-aging effect and gene expression profiling of aged rats treated with G. bimaculatus extract. Toxicol. Res. 31, 173-180.
Bai, J., Liu, X., Lu, M. and Du, Y. 2019. Characterization of genes encoding small heat shock proteins from Bemisia tabaci and expression under thermal stress. PeerJ 7, e6992.

상세보기
Kurzik-Dumke, U. and Gene, E. L. 1995. Sequence of the new Drosophila melanogaster small heat-shock-related gene, lethal (2) essential for life [l(2)efl], at locus 59F4, 5. Gene 154, 171-175.

상세보기
Kwon, K., Song, J. H., Kim, H. and Kwon, O. Y. 2020. New method for mass collection of hemolymph from the cricket Gryllus bimaculatus. J. Kans. Entomol. Soc. 93, 164-168.
Lee, N., Song, J. H., Ko, Y., Kwon, K., Lee, E. R. and Kwon, O. Y. 2020. Starvation induces endoplasmic reticulum stress and autophagy in Malpighian tubules of two-spotted field crickets Gryllus bimaculatus. J. Kansas Entomol. Soc. 93, 88-96.
Price, D. P., Schilkey, F. D., Ulanov, A. and Hansen, I. A. 2015. Small mosquitoes, large implications: Crowding and starvation affects gene expression and nutrient accumulation in Aedes aegypti. Parasit. Vectors 8, 252.

상세보기
Runtuwene, L. R., et al. 2020. The lethal(2)-essential-for-life [L(2)EFL] gene family modulates dengue virus infection in Aedes aegypti. Int. J. Mol. Sci. 21, 7520.
Shinmyo, Y., Mito, T., Matsushita, T., Sarashina, I., Miyawaki, K., Ohuchi, H. and Noji, S. 2004. piggyBac-mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus. Dev. Growth Differ. 46, 343-349.

상세보기
Shinmyo, Y., Mito, T., Matsushita, T., Sarashina, I., Miyawaki, K., Ohuchi, H. and Noji, S. 2005. caudal is required for gnathal and thoracic patterning and for posterior elongation in the intermediate-germband cricket Gryllus bimaculatus. Mech. Dev. 122, 231-239.

상세보기
Song, J. H., Kwon, K., Lee, N., Shin, H., Kim, D. W., Kim, H. and Kwon, O. Y. 2019. cDNA cloning and expression analysis of troponin C from Gryllus bimaculatus (Orthoptera: Gryllidae). J. Kansas Entomol. Soc. 92, 536-548.
Song, J. H., Lee, N., Ko, Y., Lee, E. R., Kwon, K., Choi, J. Y. and Kwon, O. Y. 2018. Zinc regulates the expression of genes encoding ZIP and ZnT transporters in the Gryllus bimaculatus (Orthoptera: Gryllidae). J. Kansas Entomol. Soc. 91, 248-252.
Stucki, D., Freitak, D., Bos, N. and Sundstrom, L. 2019. Stress responses upon starvation and exposure to bacteria in the ant Formica exsecta. PeerJ 7, e6428.

상세보기
Watanabe, T., Noji, S. and Mito, T. 2017. Genome editing in the cricket, Gryllus bimaculatus. Methods Mol. Biol. 1630, 219-233.

상세보기
Yoshimura, A., Nakata, A., Mito, T. and Noji, S. 2006. The characteristics of karyotype and telomeric satellite DNA sequences in the cricket, Gryllus bimaculatus (Orthoptera, Gryllidae). Cytogenet. Genome Res. 112, 329-336.

상세보기
Zhang, D. W., Xiao, Z. J., Zeng, B. P., Li, K. and Tang, Y. L. 2019. Insect behavior and physiological adaptation mechanisms under starvation stress. Front. Physiol, 10, 163.

상세보기
Zhang, L., Luo, L. and Jiang, X. 2008. Starvation influences allatotropin gene expression and juvenile hormone titer in the female adult oriental armyworm, Mythimna separata. Arch. Insect Biochem. Physiol. 68, 63-70.

상세보기

이 논문을 인용한 문헌

저자의 다른 논문 :

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[국내논문] 쌍별귀뚜라미(Gryllus bimaculatus)의 l(2)efl cDNA 클로닝과 발현분석
Lethal (2) Essential for Life [l(2)efl] Gene in the Two-spotted Cricket, Gryllus bimaculatus (Orthoptera: Gryllidae) 원문보기

초록
AI-Helper

Abstract ▼ AI-Helper

Keyword

표/그림 (3)

표/그림 (3)

AI 본문요약
AI-Helper

성능/효과

후속연구

참고문헌 (17)

이 논문을 인용한 문헌

저자의 다른 논문 :

활용도 분석정보

활용도 Top5 논문

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

[국내논문] 쌍별귀뚜라미(Gryllus bimaculatus)의 l(2)efl cDNA 클로닝과 발현분석 Lethal (2) Essential for Life [l(2)efl] Gene in the Two-spotted Cricket, Gryllus bimaculatus (Orthoptera: Gryllidae) 원문보기

초록 용어보기논문에서 용어와 풀이말을 자동 추출한 결과로, 시범 서비스 중입니다. AI-Helper

Abstract ▼ AI-Helper

Keyword

표/그림 (3)

표/그림 (3)

AI 본문요약 엑셀 다운로드 AI-Helper

성능/효과

후속연구

참고문헌 (17)

이 논문을 인용한 문헌

저자의 다른 논문 :

권오유 (67)

활용도 분석정보

활용도 Top5 논문 더보기

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

[국내논문] 쌍별귀뚜라미(Gryllus bimaculatus)의 l(2)efl cDNA 클로닝과 발현분석
Lethal (2) Essential for Life [l(2)efl] Gene in the Two-spotted Cricket, Gryllus bimaculatus (Orthoptera: Gryllidae) 원문보기

초록
AI-Helper

AI 본문요약
AI-Helper

활용도 Top5 논문