[논문]수면 장애 및 아토피피부염에 효과적인 7 가지 한약 처방의 항염증 활성 연구

최민진; 응웬리; 정정화; 박민영; 윤주희; 이병욱; 양인준; 신흥묵

doi:10.15188/kjopp.2021.02.35.2.56

수면 장애 및 아토피피부염에 효과적인 7 가지 한약 처방의 항염증 활성 연구
Study of Anti-inflammatory Activity of 7 Herbal Prescription Effective for Sleep Disorders and Atopic Dermatitis 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.35 no.2, 2021년, pp.56 - 63

최민진 (동국대학교 한의과대학 생리학교실) , 응웬리 (동국대학교 한의과대학 생리학교실) , 정정화 ((주)동제메디칼) , 박민영 (동국대학교 한의과대학) , 윤주희 (동국대학교 한의과대학) , 이병욱 ((주)동제메디칼) , 양인준 (동국대학교 한의과대학 생리학교실) , 신흥묵 (동국대학교 한의과대학 생리학교실)

Abstract ▼ AI-Helper

This study was conducted to find a candidate prescription with anti-inflammatory efficacy of 7 herbal prescriptions known to be effective in atopic dermatitis and sleep disorders in Korean medicine. The anti-inflammatory of the 7 herbal prescriptions extracts were evaluated by ELISA assay and Western blot assay. 7 herbal prescriptions, Seongyutang(SU), Danseontoetang(DST), Sotosajahwan(ST), Jisiljagyagsan(JJ), Seokchangpo(SCP), Wiryeongseon(WLS), Gogojohwan(GGJ) 30 % EtoH extract was used for in vitro experiments. In TNF-α + IFN-γ(TI) stimulated HaCaT cells, all 7 herbal prescriptions reduced TARC and MDC production, and JJ strongly reduced TARC and MDC production at 100 ㎍/ml concentration. SCP strongly reduced MDC production at 10 ㎍/ml and 100 ㎍/ml concentration. In addition, in substance P(SP)/CRH stimulated HMC-1 cells, JJ strongly inhibited VEGF production at both 10 and 100 ㎍/ml concentrations. In LPS/CRH stimulated Raw264.7 cells, all 7 herbal prescriptions significantly inhibited TNF-α. PMA + Ionomycin(PI)/CRH stimulated EL4 cells, SU significantly reduced IL-4 production at both concentrations of 10 and 100 ㎍/ml. WLS significantly reduced IL-17 production at both concentrations of 10 and 100㎍/ml. This suggests that 7 herbal prescriptions have anti-inflammatory effects by reducing inflammatory cytokines in keratinocytes, mast cells and macrophages caused by inflammation. Therefore, it is expected that 7 herbal prescriptions that are effective for sleep disorders and atopic dermatitis can be used as therapeutic materials for sleep disorders and diseases associated with atopic dermatitis.

주제어

표/그림 (5)

표 Table 1. Most related Effects of Prescription with Atopic Dermatitis and Sleep problems from the Database
그림 Fig. 1. Effect of prescription on the MDC and TARC expressions in TI-stimulated HaCaT cells. (A) HaCaT cells were treated with 10, 100, 500, 1000 ㎍/ml of prescription extracts for 24h. Cell viability was assessed using XTT assay. (B,C) HaCaT cells were 1h pre-treated with prescription extracts (10, 100 ㎍/ml) and DEX 10㎛, then stimulated with TNF-α, INF-γ 10 ng/ml each for 24h. Levels of MDC and TARC in culture supernatants were measured by ELISA assay. (#p < 0.05 vs. NC *p <0.05 vs TI treated group)
그림 Fig. 2. Effect of prescription on the VEGF expressions in SP and CRH-stimulated HMC-1 cells. (A) HMC-1 cells were treated with 10, 100, 500, 1000 ㎍/ml of prescription extracts for 24h. Cell viability was assessed using XTT assay. (B) HMC-1 cells were 1h pre-treated with prescription extracts 10, 100 ㎍/ml and DEX 10㎛ then stimulated with SP 10㎛ for 48h. next day, stimulated with CRH 1nM for 24h. Levels of VEGF in culture supernatants were obtained by centrifuging the medium at 1,500 rpm for 10 minutes is used measured by ELISA assay. (#p < 0.05 vs. NC *p <0.05 vs SP and CRH treated group).
그림 Fig. 3. Effect of prescription on the TNF-α expressions in LPS and CRH-stimulated Raw264.7 cells. (A) Raw264.7 cells were treated with 10, 100, 500, 1000 ㎍/ml of prescription extracts for 24h. Cell viability was assessed using XTT assay. (B) Raw264.7 cells were 1h pre-treated with prescription extracts 10, 100 ㎍//ml and DEX 10㎛ then stimulated with LPS 1ng/ml for 24h. after 1h, stimulated with CRH 1nM for 24h. Levels of MDC and TARC in culture supernatants were measured by ELISA assay. (#p < 0.05 vs. NC *p <0.05 vs LPS and CRH treated group).
그림 Fig. 4. Effect of prescription on the IL-4 and IL-17 expressions in PI and CRH-stimulated EL4 cells. (A) EL4 cells were treated with 10, 100, 500, 1000 ㎍/ml of prescription extracts for 24h. Cell viability was assessed using XTT assay. (B,C) EL4 cells were 1h pre-treated with prescription extracts 10, 100 ㎍/ml and DEX 10㎛ then stimulated with PMA 10 ng/ml, Ionomycin 100 ng/ml each for 24h. after 1h, stimulated with CRH 10nM for 24h. Levels of IL-4 and IL-17 in culture supernatants were measured by ELISA assay. (#p < 0.05 vs. NC *p <0.05 vs PI and CRH treated group).

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