[논문]파장별 레이저 자극과 전침 결합 시술이 신경병리성 동통에 미치는 영향

조명래; 김명훈; 문영민; 정성호; 나창수

doi:10.14406/acu.2021.027

[국내논문] 파장별 레이저 자극과 전침 결합 시술이 신경병리성 동통에 미치는 영향
Combined Effects of Different Wavelength Laser Stimulations and Electroacupuncture on Neuropathic Pain 원문보기

Korean journal of acupuncture, v.38 no.3, 2021년, pp.151 - 161

조명래 (동신대학교 한의과대학 침구의학교실) , 김명훈 (동신대학교 한의과대학 경혈학교실) , 문영민 (포항산업과학연구원) , 정성호 (광주과학기술원) , 나창수 (동신대학교 한의과대학 경혈학교실)

Abstract ▼ AI-Helper

Objectives : The effects of a combined stimulation of 658 nm, 830 nm, 904 nm, and 1064 nm laser acupuncture treatment (LAT) and electroacupuncture treatment (EAT) on GB39 and GB34 on neuropathic pain in rats induced by tibial and sural nerve transection were studied in this paper. Methods : To express a neuropathic pain model, surgery was performed to transection rats' tibial and sural nerves. The rats were divided into normal group, control group, and experimental groups. In addition, the experimental groups were divided into 658 nm laser and electroacupuncture (LAT658+EAT), 830 nm laser and electroacupuncture (LAT830+EAT), 904 nm laser and electroacupuncture (LAT904+EAT), and 1064 nm laser and electroacupuncture (LAT830+EAT). For the treatment of the experimental groups, electroacupuncture and different laser wavelengths were alternately applied to GB34 and GB39 twice a week for 3 weeks for 1 minute 30 seconds. The withdrawal response of neuropathic rats' legs by acetone stimulation was observed, as well as the c-Fos in the central gray region in the midbrain of neuropathic rats together with Bax, Bcl2, and mGluR5 expressions associated with apoptosis. Results : Compared with the control group, a significant decrease in the frequency of paw withdrawal in response to acetone allodynia was observed in LAT658+EAT and LAT830+EAT groups in 6th times, LAT904+EAT group in 2nd, 3rd, and 6th times, and LAT1064+EAT group in 2nd and 6th times, respectively. For c-fos positive cells in the central gray region, a significant decrease was observed in LAT830+EAT, LAT904+EAT, and LAT1064+EAT groups in comparison with the control group. In Bax expression, LAT1064+EAT group showed a significant decrease compared to the control group. In Bcl-2 expression, the LAT658+EAT,the LAT904+EAT, and the LAT1064+EAT groups increased significantly compared to the control group. LAT830+EAT, LAT904+EAT, and LAT1064+EAT groups showed significantly increased mGlu5 expression compared to the control group. Conclusions : The combination of laser for each wavelength and electroacupuncture alternately performed in this study is thought to be effective in improving neuropathic pain and apoptosis.

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표/그림 (9)

그림 Fig. 1. The procedure of experimental processing for the researching laser for each wavelength band and electroacupuncture treatment.
표 Table 1. The specifications of laser for each wavelength and electroacupuncture system
그림 Fig. 2. Effects of laser for each wavelength and electroacupuncture at acupoint GB34․GB39 on withdrawal response to cold allodynia of neuropathic pain rats induced by tibial and sural nerve transection. The data are expressed as mean±SE. LAT658+EAT : laser acupuncture therapy (658 nm) and electro-acupuncture therapy group alternately at GB34·GB37 after neuropathic pain induced by tibial and sural nerve transection. LAT830+EAT : laser acupuncture therapy (830 nm) and electro-acupuncture therapy group alternately at GB34·GB37 after neuropathic pain induced by tibial and sural nerve transection. LAT904+EAT : laser acupuncture therapy (904 nm) and electro-acupuncture therapy group alternately at GB34·GB39 after neuropathic pain induced by tibial and sural nerve transection. LAT1064+EAT : laser acupuncture therapy (1064 nm) and electro-acupuncture therapy group alternately at GB34·GB39 after neuropathic pain induced by tibial and sural nerve transection. #p<0.05 as compared with the normal group. *p<0.05, **p<0.01 as compared with the control group.
그림 Fig. 3. Effects of laser for each wavelength and electroacupuncture at acupoint GB34․GB39 on the number of c-Fos positive cells in the central gray of brain of neuropathic pain rats induced by tibial and sural nerve transection. The data are expressed as mean±SE. For groups, refer to Fig. 2. ##p<0.01 as compared with the normal group. *p<0.05, **p< 0.01 as compared with the control group.
그림 Fig. 4. Effects of laser for each wavelength and electroacupuncture at acupoint GB34․GB39 on the c- Fos-positive cells in the central gray of brain of neuropathic pain rats induced by tibial and sural nerve transection. For groups, refer to Fig. 2.
그림 Fig. 5. Effects of laser for each wavelength and electroacupuncture at acupoint GB34․GB39 on the intensity of Bax in the central gray of brain of neuropathic pain rats induced by tibial and sural nerve transection. The data are expressed as mean±SE. For groups, refer to Fig. 2. #p<0.05 as compared with the normal group. *p<0.05 as compared with the control group.
그림 Fig. 6. Effects of laser for each wavelength and electroacupuncture at acupoint GB34․GB39 on the intensity of Bcl-2 in the central gran of brain of neuropathic pain rats induced by tibial and sural nerve transection. The data are expressed as mean±SE. For groups, refer to Fig. 2. ##p<0.01 as compared with the normal group. *p<0.05, **p< 0.01 as compared with the control group.
그림 Fig. 7. Effects of laser for each wavelength and electroacupuncture at acupoint GB34․GB39 on the Bax/Bcl-2 ratio in the central gray of brain of neuropathic pain rats induced by tibial and sural nerve transection. The data are expressed as mean±SE. For groups, refer to Fig. 2. ##p<0.01 as compared with the normal group. *p<0.05, **p< 0.01 as compared with the control group.
그림 Fig. 8. Effects of laser for each wavelength and electroacupuncture at acupoint GB34․GB39 on the intensity of mGlu5 in the central gray of brain of neuropathic pain rats induced by tibial and sural nerve transection. The data are expressed as mean±SE. For groups, refer to Fig. 2. ##p<0.01 as compared with the normal group. *p<0.05 as compared with the control group.

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