[논문]속단(續斷) 추출물의 C57BL/6 마우스를 이용한 2주 경구투여 독성시험

허혜윤; 신동호; 이지혜; 서윤수; 김용범; 신인식; 강소희; 손미경; 김중선

doi:10.6116/kjh.2021.36.5.101.

속단(續斷) 추출물의 C57BL/6 마우스를 이용한 2주 경구투여 독성시험
Two-weeks Oral Dose Toxicity Study of Dipsacus asperoides Extracts in C57BL/6 Mice 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.36 no.5, 2021년, pp.101 - 108

허혜윤 (조선대학교 치과대학) , 신동호 (전남대학교 수의과대학) , 이지혜 (세명대학교 한의과대학) , 서윤수 (한국한의학연구원 한약자원연구센터) , 김용범 (안전성평가연구소) , 신인식 (전남대학교 수의과대학) , 강소희 (전남대학교 수의과대학) , 손미경 (조선대학교 치과대학) , 김중선 (전남대학교 수의과대학)

Abstract ▼ AI-Helper

Objectives : A root of Dipsacus asperoides C. Y. Cheng et T. M. Ai (D. asperoides) has been traditionally used as a medicinal resource in several Asian countries, including Korean and traditional Chinese medicine that has been traditionally used for treating several medical conditions including pain, arthritis, and bone fractures in Korea. In the present study, we investigated potential subacute toxicities of D. asperoides extract. Methods : C57BL/6 mice (male, 7weeks) were randomly divided into 4 groups of 5 mice. Except for the control group, the mice were orally administrated D. asperoides extract at doses of 50, 150, or 450 mg/kg/day for 2 weeks. At the end of the treatment period, all mice were euthanized, and the following parameters were examined: mortality, body weight, clinical signs, gross findings, hematology, serum biochemistry, organ weight, and histopathology. Results : There were no abnormalities in mortality, clinical signs, body weight, gross findings, or organ weight after repeated administration of D. asperoides extract for 2 weeks, compared with the control group. In addition, there were no significant changes in hematological, serum biochemical, and histopathological parameters between the control group and D. asperoides extract administrated groups with doses of up to 450 mg/kg/day. Conclusion : In this study, D. asperoides extract showed no significant toxicities at a dose of up to 450 mg/kg/day in mice. Although we could not confirm the toxic dose of D. asperoides extract, it can be considered safe for further pharmacological use.

주제어

표/그림 (7)

그림 Figure 1. Main target, efficacy and prescription in Traditional Korean Medicine correlation network of Dipsacus asperoides using Oriental Medicine Advanced Searching Integrated System (OASIS).
그림 Figure 2. Body weights of mice repeated administrated with D. asperoides extracts for 2 weeks. Values are expressed as means ± SD of five mice at sacrifice.
그림 Figure 3. Organ weights of mice orally administration with D. asperoides extracts for 2 weeks. (a) lung weight, (b) liver weight, (c) spleen weight, (d) kidney weight, (e) testis weight. Values are expressed as means ± SD of five mice at sacrifice.
표 Table 1. Mortality in mice after 2 weeks of repeated oral administration of D. asperoides
그림 Figure 4. Histopathological analysis of the liver in mice exposed to repeated D. asperoides extract doses of 0, 50, 150 and 450 mg/kg/day. Representative images (H&E stained) showing histological changes in the liver of 0, 50, 150 and 450 mg/kg/day-treated mice samples.
표 Table 2. Hematological analysis in mice after 2 weeks of repeated oral administration of D. asperoides
표 Table 3. Serum biochemical values in mice after 2 weeks of repeated oral administration of D. asperoides

참고문헌 (21)

Korea Institute of Oriental Medicine. Defining Dictionary for Medicinal Herbs[Korean, 'Hanyak Giwon Sajeon'](2021). Published on the Internet; https://oasis.kiom.re.kr/herblib/hminfo/hbmcod/hbmcodList.do (accessed 2021-05-31).
Textbook Compilation Committee of National Universitiy of Korean Medicine. Herbology. Seoul: Yeonglimsa. 2007 : 605-6.
Tan LH, Zhang D, Yu B, Zhao SP, Wang JW, Yao L, Cao WG. Antioxidant activity and optimization of extraction of polysaccharide from the roots of Dipsacus asperoides. Int. J. Biol. Macromol. 2015 ; 81 : 332-9.

상세보기
Min JY, Park YK. Effect of Dipsaci Radix Water Extract on LPS-induced Inflammatory Response in RAW264.7 Mouse Macrophages. Kor. J. Herbology. 2009 ; 24(4):189-95.
Cong G, Cui L, Zang M, Hao L. Attenuation of renal ischemia/reperfusion injury by a polysaccharide from the roots of Dipsacus asperoides. Int. J. Biol. Macromol. 2013 ; 56 : 14-9.

상세보기
Jung HW, Jung JK, Son KH, Lee DH, Kang TM, Kim YS, Park YK. Inhibitory effects of the root extract of Dipsacus asperoides C.Y. Cheng et al T.M.Ai on collagen-induced arthritis in mice. J. Ethnopharmacol. 2012 ; 139(1) : 98-103.

상세보기
He J, Li X, Wang Z, Bennett S, Chen K, Xiao Z, Zhan J, Chen S, Hou Y, Chen J, Wang S, Xu J, Lin D. Therapeutic Anabolic and Anticatabolic Benefits of Natural Chinese Medicines for the Treatment of Osteoporosis. Front. Pharmacol. 2019 ; 10 : 1344,

상세보기
Li H, Zhang D, Tan LH, Yu B, Zhao SP, Cao WG. Comparison of the antioxidant properties of various solvent extracts from Dipsacus asperoides and identification of phenolic compounds by LC-ESI-QTOF-MS-MS. S. Afr. J. Bot. 2017 ; 109 : 1-8.
Ling Y, Liu K, Zhang Q, Liao L, Lu Y. High performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight-mass spectrometry as a powerful analytical strategy for systematic analysis and improved characterization of the major bioactive constituents from Radix Dipsaci. J. Pharm. Biomed. Anal. 2014 ; 98 : 120-129.

상세보기
Naturalendo tech Corp. Compositions for Improving Skin Conditions Comprising Extract of Phlomis umbrosa. 10-2010-0006585 (Patent) 2010.
Brainon Corp. A composition for promoting exercise activity and muscle comprising a Dipsaci Radix and a Cynanchum wilfordii Radix extract. 10-2015-0098183 (Patent) 2015.
Shin IS, Lee MY, Kim Y, Seo CS, Kim JH, Shin HK. Subacute toxicity and stability of Sosiho-tang, a traditional herbal formula, in Sprague-Dawley rats. BMC Complement. Alter. Med. 2012 ; 12 : 266.

상세보기
Shin NR, Lee AY, Park G, Ko JW, Kim JC, Shin IS, Kim JS. Therapeutic effect of Dipsacus asperoides C. Y. Cheng et T. M. Ai in ovalbumin-induced murine model of asthma. Int. J. Mol. Sci. 2019 ; 20(8) : 1855.

상세보기
Park JY, Park SD, Koh YJ, Kim DI, Lee JH. Aqueous extract of Dipsacus asperoides suppresses lipopolysaccharide-stimulated inflammatory responses by inhibiting the ERK1/2 signaling pathway in RAW 264.7 macrophages. J. Ethnopharmacol. 2019 ; 231 : 253-61.

상세보기
Jung HW, Jung JK, Son KH, Lee DH, Kang TM, Kim YS, Park YK. Inhibitory effects of the root extract of Dipsacus asperoides C.Y. Cheng et al T.M.Ai on collagen-induced arthritis in mice. J. Ethnopharmacol. 2012 ; 139(1) : 98-103.

상세보기
Bae YH, Huh JI, Kwack SJ, Seok JH, Lee JK, Kang TS, Woo MH, Choi JS, Min BS. Analysis and Stability Test of the Extracts from Dipsaci Radix, Leonuri Herba and Cyperi Rhizoma for Toxicity Study. Kor. J. Pharmacogn. 2012 ; 43(1) : 79-84.
Kim JC, Shin DH, Kim SH, Kim JK, Park SC, Son WC, Lee HS, Suh JE, Kim CY, Ha CS, Chung MK. Subacute toxicity evaluation of a new camptothecin anticancer agent CKD-602 administered by intravenous injection to rats. Regul. Toxicol. Pharmacol. 2004 ; 40(3) : 356-69.

상세보기
Chun JM, Lee AY, Nam JY, Lim KS, Choe MS, Lee MY, Kim C, Kim JS. Effects of Dipsacus asperoides extract on monosodium lodoacetate-induced osteroarthritis in rats based on gene expression profiling. Front. Pharmacol. 2021 ; 12 : 615157.

상세보기
Lee MY, Shin IS, Seo CS, Kim JH, Han SR, Shin HK. Subchronic oral toxicity studies of the traditional herabl formula Bangpungtongseong-san in Crl: CD (SD) rats. J. Ethnopharmacol. 2012 ; 144 : 720-25.19.

상세보기
Niu Y, Li C, Pan Y, Li Y, Kong X, Wang S, Zhai Y, Wu X, Fan W, Mei Q. Treatment of radix dipsaci extract prevents bone loss induced by modeled microgravity in hindlimb unloading rats. Pharm. Biol. 2015 ; 53(1) : 110-6.

상세보기
Wozniak DM, Kirchoff N, Hansen-Kant K, Sogoba N, Safronetz D, Prescott J. Hematology and Clinical Chemistry Reference Ranges for Laboratory-Bred Natal Multimammate Mice (Mastomys natalensis). Viruses. 2021 ; 13(2) : 187.

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

속단(續斷) 추출물의 C57BL/6 마우스를 이용한 2주 경구투여 독성시험
Two-weeks Oral Dose Toxicity Study of Dipsacus asperoides Extracts in C57BL/6 Mice 원문보기

Abstract ▼ AI-Helper

주제어

표/그림 (7)

표/그림 (7)

참고문헌 (21)

이 논문을 인용한 문헌

저자의 다른 논문 :

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

속단(續斷) 추출물의 C57BL/6 마우스를 이용한 2주 경구투여 독성시험 Two-weeks Oral Dose Toxicity Study of Dipsacus asperoides Extracts in C57BL/6 Mice 원문보기

Abstract ▼ AI-Helper

주제어

표/그림 (7) 모든 표/그림 보기

표/그림 (7) 슬라이드로 보기

참고문헌 (21)

이 논문을 인용한 문헌

저자의 다른 논문 :

신동호 (46) 신인식 (10) 손미경 (37) 김중선 (12)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

속단(續斷) 추출물의 C57BL/6 마우스를 이용한 2주 경구투여 독성시험
Two-weeks Oral Dose Toxicity Study of Dipsacus asperoides Extracts in C57BL/6 Mice 원문보기

표/그림 (7)

표/그림 (7)