[논문]개똥쑥 추출물의 항여드름 효능확인

유지영; 노경백; 오세영; 정용택; 박덕훈; 정은선

doi:10.15230/scsk.2021.47.3.247

개똥쑥 추출물의 항여드름 효능확인
Anti-acne Properties of Artemisia annua Extract In Vitro 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.47 no.3, 2021년, pp.247 - 254

유지영 (바이오스펙트럼(주) 생명과학연구소) , 노경백 (바이오스펙트럼(주) 생명과학연구소) , 오세영 (바이오스펙트럼(주) 생명과학연구소) , 정용택 (바이오스펙트럼(주) 생명과학연구소) , 박덕훈 (바이오스펙트럼(주) 생명과학연구소) , 정은선 (바이오스펙트럼(주) 생명과학연구소)

초록
AI-Helper

여드름은 털피지샘단위(pilosebaceous unit)와 관련된 만성 염증 피부 질환으로, 여드름 병변에서는 피지 과다분비(hyperseborrhea)나 이상분비(dysseborrhea), 염증반응, 그리고 다른 피부상재균들에 비해 증가된 Cutibacterium acnes (C. acnes)로 인한 피부 미생물 균총의 불균형이 관찰된다. 이 연구는 개똥쑥 추출물(Artemisia annua extract: AAE)의 항여드름 효과를 확인한 것으로, 피부 미생물 균총의 균형유지와 관련된 항균효과와 염증반응의 억제, 과도한 피지 분비의 감소 측면에서 실험을 진행하였다. C. acnes와 Staphylococcus epidermidis(S. epidermidis)를 AAE와 공동배양 하였을 때, S. epidermidis의 성장률은 저해되지 않았지만 C. acnes의 성장률은 저해된 것을 확인하였다. 또한 AAE를 처리하여 배양한 C. acnes 배양배지를 세포에 처리하였을 때, 인터루킨-1β(IL-1β), 종양괴사인자-α(TNF-α)와 인터루킨-6(IL-6) 같은 사이토카인 분비의 감소와 TLR2 활성 억제도 확인하였다. 마지막으로 피지세포에 AAE를 처리한 결과, 팔미트산에 의해 유도된 피지형성을 감소시키는 것을 확인하였다. 이 결과들은 AAE가 다양한 타깃을 지닌 천연추출물로써 여드름의 주요 원인들인 C. acnes의 선택적 성장저해와 C. acnes로부터 유도되는 염증반응을 억제할 수 있으며, 과도한 피지형성을 감소시켜 결과적으로 여드름을 완화시키는 물질로 사용될 수 있다는 것을 제시한다.

Abstract ▼ AI-Helper

Acne vulgaris is a chronic inflammatory skin disease related to pilosebaceous unit. In acne lesions, hyperseborrhea, dysseborrhea, inflammatory event, and an imbalance in skin microflora, particularly an increase in Cutibacterium acnes (C. acnes) colonization comparing to other bacteria, have been observed. The objective of this study was to evaluate anti-acne effects of Artemisia annua extract (AAE) on antibacterial activity related to preservation of the balance in skin microbiome, inhibition of inflammation, and reduction of excessive sebum production. When C. acnes and Staphylococcus epidermidis (S. epidermidis) were co-cultured in the presence of AAE, the reduction of C. acnes growth by AAE was greater than that of S. epidermidis. In addition, when C. acnes was cultured in a medium containing AAE (C. acnes AAE), levels of cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-6 and toll-like receptors-2 activity were decreased in comparison with C. acnes cultured in a medium without AAE (C. acnes CM). Moreover, AAE significantly inhibited excessive sebum production induced by palmitic acid. These results suggest that AAE, as a natural extract with various targets, can inhibit selective growth of C. acnes and inflammatory reactions derived from C. acnes, which are the main causes of acne, and consequently can be used as a substance to alleviate acne by reducing excessive sebum formation.

주제어

표/그림 (4)

그림 Figure 1. A. annua extract inhibits the growth of C. acnes selectively. S. epidermidis (1 × 10⁴ CFU/mL) and C. acnes (1 × 10⁶ CFU/mL) were co-cultured in the presence or absence of AAE under anaerobic conditions for two days at 37 ℃. Data are expressed as mean ± SD, *p < 0.05 compared to the control.
그림 Figure 2. A. annua extract regulates C. acnes CM-induced TLR2 signaling pathways. (A) TLR2-HEK293 cells were cultured in the presence of C. acnes CM with or without AAE diluted to one hundredth in media. TLR2 signal activation was then measured using a Quanti-blue solution. (B) Cell viability of TLR2-HEK293 was detection by WST-1 assay. Data are expressed as mean ± SD, *p < 0.001 versus C. acnes CM untreated control; **p < 0.01 versus C. acnes CM treated control.
그림 Figure 3. A. annua extract inhibits the expression of proinflammatory cytokines in THP-1 cells induced by C. acnes culture medium. THP-1 cells were cultured in the presence of C. acnes CM with or without AAE diluted to one hundredth in media. Secreted proinflammatory cytokines such as (A) IL-1β, (B) TNF-α and (C) IL-6 from THP-1 cells were then measured by ELISA. (D) Cell viability of THP-1 was detection by MTT assay. Values are expressed as mean ± SD, *p < 0.001 versus C. acnes CM untreated control. **p < 0.01 versus C. acnes CM treated control.
그림 Figure 4. A. annua extract suppresses sebum accumulation induced by palmitic acid in sebocytes. Sebocytes were cultured in DMEM in the presence of palmitic acid with or without AAE. Sebum synthesized in sebocytes was detected by Nile red staining. Scale bars, 100 μm. Values are expressed as mean ± SD, *p < 0.001 versus palmitic acid untreated control, **p < 0.001 versus palmitic acid treated control.

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