[논문]쌀겨오일의 안티폴루션 화장품 소재로써의 응용

강해란; 정소영; 허효진; 차병선; 이소민; 여혜림; 유경완; 곽준수; 곽병문; 이미기; 빈범호

doi:10.15230/scsk.2021.47.3.237

쌀겨오일의 안티폴루션 화장품 소재로써의 응용
Application of Oryza sativa (Rice) Bran Oil as an Anti-pollution Cosmetic Material 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.47 no.3, 2021년, pp.237 - 245

강해란 (아주대학교 응용생명공학과) , 정소영 (아주대학교 응용생명공학과) , 허효진 (아주대학교 응용생명공학과) , 차병선 (아주대학교 응용생명공학과) , (아주대학교 응용생명공학과) , 이소민 (아주대학교 응용생명공학과) , 여혜림 (아주대학교 응용생명공학과) , 유경완 (아주대학교 응용생명공학과) , 곽준수 (아주대학교 응용생명공학과) , 곽병문 (세명대학교 화장품뷰티생명공학부) , 이미기 (경기도경제과학진흥원) , 빈범호 (아주대학교 응용생명공학과)

초록
AI-Helper

미세먼지와 초미세먼지는 일반적으로 사람 머리카락의 두께에 비하여 각각 1/6 ~ 1/7, 1/20 ~ 1/30 정도로 매우 작은 부유성 먼지를 뜻하며, 다양한 종류의 중금속 이온이 내포되어 있다. 호흡뿐 아니라 피부의 모공 틈새를 통해서 유입된 미세먼지는 체내 조직과 피부 건강에 문제를 유발할 수 있기 때문에 유입을 막거나 깨끗이 씻어내어 제거해야 한다. 본 연구를 통해 Oryza sativa (Rice) bran oil (OSBO, 쌀겨오일)을 이용하면 중금속 이온을 흡착하여 제거할 수 있을 것이라는 가능성을 확인하였다. 또한, 세포독성 실험을 통해 세포 생존율이 곡물 유래 성분과 비교했을 때에도 월등히 높아 외부 자극원에 대한 세포 보호 효과를 기대할 수 있다. COL1A1 mRNA의 발현량이 높아짐을 확인하였고, 이에 따라 미세먼지 속 중금속 이온에 의해 손실된 수분으로 유발될 수 있는 주름을 완화할 수 있을 것으로 사료된다. 이러한 실험 결과를 바탕으로 최종적으로 중금속 제거를 위한 워시-오프 제형의 OSBO 함유 화장품을 제시하고자 하였다.

Abstract ▼ AI-Helper

Particulate matter and ultra-particle matters generally refer to very small floating dust, such as 1/6 to 1/7 and 1/20 to 1/30, respectively, compared to the thickness of human hair, and contain various types of heavy metal ions. In addition to breathing, particle matters (PM) that flows in through the gaps in the pores of the skin can induce health problems in the body's tissues and skin, so it must be removed by blocking the inflow or by washing. Through this study, we confirmed the possibility that heavy metal ions can be adsorbed and removed by using Oryza sativa (Rice) bran oil (OSBO). In addition, the cell viability is much higher than that of grain-derived components through cytotoxicity experiments, and the cytoprotective effect of an external stimulus source can be expected. It was confirmed that the expression amount of COL1A1 mRNA increased, and accordingly, it was believed that wrinkles that might be caused by moisture lost by heavy metal ions in fine dust could be alleviated. Based on the results of these experiments, we tried to present a cosmetics containing OSBO, which is a wash-off formulation, in order to finally remove heavy metals.

주제어

표/그림 (9)

표 Table 1. Information of Test Samples
표 Table 2. Sequence of Primers
표 Table 3. Composition of Cosmetic Product Containing OSBO
그림 Figure 1. Heavy metal adsorption capacity test results. As a result of comparing the color intensity using the heavy metal solution, it can be known that OSBO adsorbs heavy metal ions. (A) Comparison results by concentration of heavy metal solution. (B) Comparison of Image J calculated values by type and concentration of heavy metal solution. CTL means control, it was not treated with OSBO. The results are presented as the mean ± SD of the experiment (N = 3), *p < 0.05, **p < 0.01, ***p < 0.005, compared to CTL.
그림 Figure 2. MTT Assay results by OSBO concentration in skin fibroblast cell. Cells were treated with OSBO (Oryza sativa (Rice) bran oil) for 24 h, 37 ℃, 5% CO₂. (A) Treated concentration of OSBO is 0, 25, 50, 100 ng/mL. (B) Treated concentration of OSBO is 0, 10, 100, 1,000 ng/mL. CTL means control, it was not treated with OSBO. The results are presented as the mean ± SD of the experiment (N = 3), *p < 0.05, **p < 0.01, ***p < 0.005, compared to CTL.
그림 Figure 3. MTT assay results by 100 ng/mL of OSBO and grain-derived components in skin fibroblast cell. Cells were treated with OSBO (Oryza sativa (Rice) bran oil), SBEE (Sorghum bicolor ethanol extract), CMEE (Coix lacryma-jobi Ma-yuen shell ethanol extract), PSREE (Polygonatum sibiricum rhizome ethanol extract) for 24 h, 37 ℃, 5% CO₂. CTL means control, it was not treated with any samples. The results are presented as the mean ± SD of the experiment (N = 3), *p < 0.05, **p < 0.01, ***p < 0.005, compared to CTL.
그림 Figure 4. Comparion results for mRNA expression level through qPCR in skin fibroblast cell. (A) The result of relative expression amount of COL1A1 to GAPDH. (B) The result of relative expression amount of IL-6 to GAPDH. Cells were treated with OSBO (Oryza sativa (Rice) bran oil), SBEE (Sorghum bicolor ethanol extract), CMEE (Coix lacryma-jobi Ma-yuen shell ethanol extract), PSREE (Polygonatum sibiricum rhizome ethanol extract) for 24 h, 37 ℃, 5% CO₂. CTL means control, it was not treated with any samples. OSBO induces COL1A1 expression and reduces IL-6 expression in skin fibroblast cell. The results are presented as the mean ± SD of the experiment (N = 3), *p < 0.05, **p < 0.01, ***p < 0.005, compared to CTL.
그림 Figure 5. Heavy metal adsorption capacity test results in formulations. As a result of comparing the color intensity using the heavy metal solution, it can be known that cosmetic product containing 0.1% OSBO adsorbs heavy metal ions. (A) Comparison results before and after using cosmetic product containing 0.1% OSBO. (B) Comparison of Image J calculated values. The results are presented as the mean ± SD of the experiment (N=3), *p < 0.05, **p < 0.01, ***p < 0.005, compared to CTL.
그림 Figure 6. The stability of cosmetic product containing 0.1% of OSBO over time. Except for freezing at 4 ℃, it was stable up to 2 months as a result of observing mainly odor, discoloration and precipitation immediately after production, 1 week, 1 month and 2 months later. Due to the characteristics of the product, it is frozen under the condition of 4 ℃, but it returns to its original state when left at room temperature (RT) for 2 h.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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