[논문]Comparison of clinical diagnostic performance between commercial RRT-LAMP and RT-qPCR assays for SARS-CoV-2 detection

Kim, Hye-Ryung; Park, Jonghyun; Han, Hyung-Soo; Kim, Yu-Kyung; Jeon, Hyo-Sung; Park, Seung-Chun; Park, Choi-Kyu

doi:10.7853/kjvs.2021.44.3.163

Comparison of clinical diagnostic performance between commercial RRT-LAMP and RT-qPCR assays for SARS-CoV-2 detection 원문보기

韓國家畜衛生學會誌 = Korean journal of veterinary service, v.44 no.3, 2021년, pp.163 - 168

Kim, Hye-Ryung (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) , Park, Jonghyun (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) , Han, Hyung-Soo (Department of Physiology, School of Medicine, Kyungpook National University) , Kim, Yu-Kyung (Department of Clinical Pathology, School of Medicine, Kyungpook National University) , Jeon, Hyo-Sung (M Monitor Incorporation) , Park, Seung-Chun (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) , Park, Choi-Kyu (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University)

Abstract ▼ AI-Helper

The rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in isolating infected patients and preventing further viral transmission. In this study, we evaluated the clinical diagnostic performances of a commercial real-time reverse transcription loop-mediated isothermal amplification (RRT-LAMP) assay (Isopollo® COVID-2 assay, M-monitor, Daegu, Korea) using eighty COVID-19 suspected clinical samples and compared these with the results of a commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) assay (AllplexTM 2019-nCoV rRT-QPCR Assay, SeeGene, Seoul, Korea). The results of the RRT-LAMP assay targeting the N or RdRp gene of SARS-CoV-2 showed perfect agreement with the RT-qPCR assay results in terms of detection. Furthermore, the RRT-LAMP assay was completed in just within a 20-min reaction time, which is significantly faster than about the 2 h currently required for the RT-qPCR assay, thus enabling prompt decision making regarding the isolation of infected patients. The RRT-LAMP assay will be a valuable tool for rapid, sensitive, and specific detection of SARS-CoV-2 in human or unexpected animal clinical cases.

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표/그림 (2)

표 Table 1. Comparison of clinical performance between mRRT-LAMP and sgRT-qPCR for the detection of SARS-CoV-2
표 Table 2. Comparison of reaction times between mRRT-LAMP and sgRT-qPCR for the detection of SARS-CoV-2 genes from clinical samples

참고문헌 (11)

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Comparison of clinical diagnostic performance between commercial RRT-LAMP and RT-qPCR assays for SARS-CoV-2 detection 원문보기

Abstract ▼ AI-Helper

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