[논문]Optimization of Medium for Lipase Production from Zygosaccharomyces mellis SG1.2 Using Statistical Experiment Design

Pramitasari, Marisa Dian; Ilmi, Miftahul

doi:10.48022/mbl.2106.06016

[국내논문] Optimization of Medium for Lipase Production from Zygosaccharomyces mellis SG1.2 Using Statistical Experiment Design 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.3, 2021년, pp.337 - 345

Pramitasari, Marisa Dian (Faculty of Biology, Universitas Gadjah Mada) , Ilmi, Miftahul (Faculty of Biology, Universitas Gadjah Mada)

Abstract ▼ AI-Helper

Lipase (triacylglycerol lipase, EC 3.1.1.3) is an enzyme capable of hydrolyzing triacylglycerol, to produce fatty acids and glycerol and reverse the reaction of triacylglycerol synthesis from fatty acids and glycerol through transesterification. Applications of lipase are quite widespread in the industrial sector, including in the detergent, paper, dairy, and food industries, as well as for biodiesel synthesis. Lipases by yeasts have attracted industrial attention because of their fast production times and high stability. In a previous study, a lipase-producing yeast isolate was identified as Zygosaccharomyces mellis SG1.2 and had a productivity of 24.56 U/mg of biomass. This productivity value has the potential to be a new source of lipase, besides Yarrowia lypolitica which has been known as a lipase producer with a productivity of 0.758 U/mg. Lipase production by Z. mellis SG1.2 needs to be increased by optimizing the production medium. The aims of this study were to determine the significant component of the medium for lipase production and methods to increase lipase production using the optimum medium. The two methods used for the statistical optimization of production medium were Taguchi and RSM (Response Surface Methodology). The data obtained were analyzed using Minitab 18 and SPSS 23 software. The most significant factors which affected lipase productivity were olive oil and peptones. The optimum medium composition consisted of 1.02% olive oil, 2.19% peptone, 0.05% MgSO4·7H2O, 0.05% KCl, and 0.2% K2HPO4. The optimum medium was able to increase the lipase productivity of Z. mellis SG1.2 to 1.8-fold times the productivity before optimization.

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표/그림 (11)

표 Table 1. Factors and levels on experimental design Taguchi.
표 Table 2. Experimental design of RSM method.
그림 Fig. 1. Mean Plot for SNR (Signal to Noise Ratios) of lipase production, (A) olive oil, (B) peptone, (C) MgSO₄.7H₂O, (D) KCl, and (E) K₂HPO₄.
그림 Fig. 2. Normal probability plot for lipase production (R-squared = 92.6%).
표 Table 3. ANOVA of Mean Plot for SNR.
그림 Fig. 3. Contour plot of combination of olive oil and peptone on lipase production.
그림 Fig. 4. Surface plot of combination of olive oil and peptone on lipase production.
그림 Fig. 5. Graph of lipase productivity predictions using response optimizer.
그림 Fig. 6. Lipase productivity profile from Z. mellis SG1.2 on basal and optimum medium.
표 Table 4. ANOVA of polynomial equation model.
표 Table 5. Comparison between the predicted and experimental productivity values.

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