제주 정수장에서 출현한 깔따구과 유충의 형태 및 유전학적 분석 Morphological and Genetic Species Identification in the Chironomidae Larvae Found in Tap Water Purification Plants in Jeju원문보기
깔따구(Diptera: Chironomidae)는 저서성 대형무척추동물로 환경 및 수질 변화에 민감한 영향을 받는 중요한 환경지표생물이다. 이러한 깔따구 유충이 정수장에서 본 연구에서는 제주 정수처리장에서 발견된 깔따구 유충의 종을 분류하기 위해 형태 사진 및 COI (cytochrome coxidase subunit I) Primer로 증폭시킨 DNA의 염기서열을 계통수 분석을 통해 분석을 실시하였다. 정수장 내 수도꼭지와 소화관 등에서 채집된 17개체는 둥근깃깔따구(O. tamarutilus) 14개체, 타마긴털깔따구(P. tammaater) 3개체 총 2종으로 확인되었다. 각 깔따구 종의 형태적 특징은 두부, 하순기절, 대악, 안테나, 발톱의 형태적 특징의 분류기로 종 동정하였다. NCBI Genbank에 등록된 깔따구 19종 COI 염기서열을 기반으로 본 연구에서 조사된 17개체의 계통진화적 분석 결과 채집된 깔따구 COI 염기서열이 둥근깃깔따구(O. tamarutilus)와 타마긴털깔따구(P. tammaater) 2종으로 각각 계통군(clade)를 이루는 것이 확인되었다. 이러한 결과는 정수장 환경별 발견되는 깔따구 유충의 다양성의 확인과 형태적-유전적 종 동정 분류정보를 바탕으로 수환경 관리 및 평가를 위한 기반 정보로 활용될 것이다.
깔따구(Diptera: Chironomidae)는 저서성 대형무척추동물로 환경 및 수질 변화에 민감한 영향을 받는 중요한 환경지표생물이다. 이러한 깔따구 유충이 정수장에서 본 연구에서는 제주 정수처리장에서 발견된 깔따구 유충의 종을 분류하기 위해 형태 사진 및 COI (cytochrome c oxidase subunit I) Primer로 증폭시킨 DNA의 염기서열을 계통수 분석을 통해 분석을 실시하였다. 정수장 내 수도꼭지와 소화관 등에서 채집된 17개체는 둥근깃깔따구(O. tamarutilus) 14개체, 타마긴털깔따구(P. tammaater) 3개체 총 2종으로 확인되었다. 각 깔따구 종의 형태적 특징은 두부, 하순기절, 대악, 안테나, 발톱의 형태적 특징의 분류기로 종 동정하였다. NCBI Genbank에 등록된 깔따구 19종 COI 염기서열을 기반으로 본 연구에서 조사된 17개체의 계통진화적 분석 결과 채집된 깔따구 COI 염기서열이 둥근깃깔따구(O. tamarutilus)와 타마긴털깔따구(P. tammaater) 2종으로 각각 계통군(clade)를 이루는 것이 확인되었다. 이러한 결과는 정수장 환경별 발견되는 깔따구 유충의 다양성의 확인과 형태적-유전적 종 동정 분류정보를 바탕으로 수환경 관리 및 평가를 위한 기반 정보로 활용될 것이다.
The Chironomidae is a benthic macroinvertebrate commonly found in freshwater ecosystems, along with Ephemeroptera and Trichoptera, which can be used for environmental health assessments. There are approximately 15,000 species of Chironomidae worldwide, but there are limited studies on species identi...
The Chironomidae is a benthic macroinvertebrate commonly found in freshwater ecosystems, along with Ephemeroptera and Trichoptera, which can be used for environmental health assessments. There are approximately 15,000 species of Chironomidae worldwide, but there are limited studies on species identification of domestic Chironomidae larvae. In the present study, we carried out species classification of the Chironomidae larvae that found in Jeju's tap water purification plants using morphological characteristics and genetic identification based on cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA. Body shape, mentum, antenna, mandible in the head capsule, and claws were observed in the larvae for morphological classification. Analysis of 17 larvae collected from faucets and fire hydrants of domestic tap water purification plants revealed the presence of two species, including 14 Orthocladius tamarutilus and 3 Paratrichocladius tammaater. These results will aid the use of the criteria information about species classification of the Chironomidae for water quality management in water purification plants and diversity monitoring of freshwater environments.
The Chironomidae is a benthic macroinvertebrate commonly found in freshwater ecosystems, along with Ephemeroptera and Trichoptera, which can be used for environmental health assessments. There are approximately 15,000 species of Chironomidae worldwide, but there are limited studies on species identification of domestic Chironomidae larvae. In the present study, we carried out species classification of the Chironomidae larvae that found in Jeju's tap water purification plants using morphological characteristics and genetic identification based on cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA. Body shape, mentum, antenna, mandible in the head capsule, and claws were observed in the larvae for morphological classification. Analysis of 17 larvae collected from faucets and fire hydrants of domestic tap water purification plants revealed the presence of two species, including 14 Orthocladius tamarutilus and 3 Paratrichocladius tammaater. These results will aid the use of the criteria information about species classification of the Chironomidae for water quality management in water purification plants and diversity monitoring of freshwater environments.
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