[논문]간접수용복 시멘트 처리로 유발된 활성산소종에 의한 치주줄기세포 독성

박소영

doi:10.13065/jksdh.20210052

간접수용복 시멘트 처리로 유발된 활성산소종에 의한 치주줄기세포 독성
Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells 원문보기

JKSDH : Journal of Korean Society of Dental Hygiene = 한국치위생학회지, v.21 no.5, 2021년, pp.545 - 553

박소영 (동서대학교 치위생학과)

Abstract ▼ AI-Helper

Objectives: This study aimed to investigate the cytotoxicity of Nexus RMGIC, an indirect restorative cement, on cell survival rate and reactive oxygen species (ROS) production in periodontal stem cells (PDSCs). Methods: PDSCs were incubated with serially diluted Nexus RMGIC eluates with and without the addition of N-acetyl-cysteine (NAC). Cell survival was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ROS generation was determined by measuring the fluorescence intensity for 2',7'-dichlorofluorescin diacetate. Results: Nexus RMGIC exposure decreased cell proliferation and cell survival rate in a dose-dependent manner (1:8, 1:4, 1:2, 1:1) in PDSCs. The cytotoxicity of Nexus RMGIC was inhibited by treatment with 10-mM NAC. In addition, the production of ROS was detected by immunofluorescence after PDSCs were exposed to Nexus RMGIC. However, ROS generation was significantly suppressed in the NAC pretreatment compared with the Nexus RMGIC group. Conclusions: Nexus RMGIC increased the cytotoxicity and ROS generation. ROS was involved in Nexus RMGIC-induced cell toxicity.

주제어

표/그림 (3)

그림 Fig. 1. The cell viability of PDSCs exposed Nexus RMGIC. PDSCs were exposed to different eluates of Nexus RMGIC (1:1-1:8) for 24 h. Representative images are shown in (A). (B) Cell viability of PDSCs exposed to Nexus RMGIC eluates analyzed by WST assay. Nexus RMGIC decreased cell viability of PDSCs in a dose-dependent manner. Data indicated as percentage relative to unexposed control group (100%). *p<0.05, **p<0.01 compared with control group (only medium).
그림 Fig. 2. ROS were involved in Nexus RMGIC-caused cytotoxicity in PDSCs. Representative images are shown in (A). (B) The cell survival rate of Nexus RMGIC in the presence or absence of 10 mM NAC was analyzed by WST assay. Data indicated as percentage relative to unexposed control group (100%). *p<0.05, **p<0.01; N.S: not significant
그림 Fig. 3. Nexus RMGIC increased ROS generation in PDSCs. Representative images of fluorescence for DCF-DA (green), a ROS probe. The exposed cells to Nexus RMGIC (1:4, 1:8) alone (A) and Nexus RMGIC (1:4, 1:8) following 10 mM NAC showed different fluorescence intensity. The statistic number of fluorescence positive cells were counted using image J.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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