[논문]제주 자생 우슬(Achyranthes japonica Nakai)의 항산화, 항염증과 항주름 평가

엄미선

doi:10.12925/jkocs.2021.38.5.1209

[국내논문] 제주 자생 우슬(Achyranthes japonica Nakai)의 항산화, 항염증과 항주름 평가
The evaluation of Anti-oxidation, Anti-inflammation and Anti-wrinkle activity of Jeju Native Achyranthes japonica Nakai 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.38 no.5, 2021년, pp.1209 - 1218

엄미선 (장안대학교 뷰티케어과)

초록
AI-Helper

본 연구는 화장품 소재로서 우슬(Achyranthes japonica Nakai)의 가능성을 확인하기 위한 것이다. 우슬 추출물이 가지고 있는 항산화, 항염증, 항주름 효과를 측정하였다. 각각의 식물 재료는 70% 에탄올을 이용하여 우슬 뿌리(Achyranthes japonica Nakai roots, AJNR)와 우슬 줄기(Achyranthes japonica Nakai stalks, AJNS)로부터 추출하였다. RAW 264.7 세포를 배양하여 추출물의 Nitric oxide assay를 진행하였고, 섬유아세포 CCD-986sk를 배양하여 추출물의 MMP-1 assay, Type I procollagen synthesis assay를 실시하였다. 이 연구의 결과, 항산화 활성은 우슬 뿌리와 우슬 줄기 모두 우수하였고, 뿌리는 함염증 효과가 월등하게 우수하였으며 줄기는 뿌리에 비해 MMP-1 저해 활성과 Type I procollagen 합성 효과가 조금 더 높았다. 이 연구의 결과, 우슬 뿌리와 우슬 줄기의 항산화 활성은 유사한 수준으로 우수하였고, 뿌리의 항염 활성은 줄기보다 월등하게 높았다. MMP-1 저해 활성과 Type I procollagen 합성 효과는 대체적으로 우수하였는데 줄기가 뿌리에 비해 가 조금 더 뛰어났다. 그러므로 우슬은 항산화, 항염증, 항주름의 활성을 갖는 기능성 화장품 소재로서 활용이 가능할 것으로 판단된다.

Abstract ▼ AI-Helper

The aim of this study was to assure the possiblility of Achyranthes japonica Nakai for a cosmetic material. For this we examined the Anti-oxidation, Anti-inflammation, Anti-wrinkle effects of Achyranthes japonica Nakai extract. Each material is extracted from Achyranthes japonica Nakai roots(AJNR) and Achyranthes japonica Nakai stalks(AJNS) using 70% ethanol. We carried out Nitric oxide assay of the extract with cultured RAW 264.7 macrophage cells, And we conducted MMP-1 assay, Type I procollagen synthesis assay of the extract with cultured CCD-986sk fibroblast cells. After we measured ABTS+ assay and SOD assay of AJNR and AJNS, we knew that effect of anti-oxidant increased as their concentration get higher. The result of this study was that the antioxidant activity of AJNR and AJNS was similarly excellent and the anti-inflammatory activity of AJNR was significantly higher than AJNS. The MMP-1 inhibitory activity and Type I procollagen synthesis of AJNR and AJNS was generally excellent, AJNS had slightly better than AJNR. Therefore I thought it has the chance to play an imfortant role as a functional cosmetic material for anti-oxidant, anti-inflammation and wrinkle reduction effects.

주제어

표/그림 (7)

그림 Fig. 1. ABTS+ cation radical scavenging activity(%) of AJNR(Achyranthes japonica Nakai root) and AJNS(Achyranthes japonica Nakai stalks) depending on concentration.. AJNS was higher in Anti-oxident effect than AJNR. Results are expressed as mean±SD of triplicate experiments.
그림 Fig. 3-2. Cell viability of depending onconcentration of AJNR and AJNSusing MTT assay(CCD-986sk). Thedata illustrate the mean±SD of threeseparate experiments.
그림 Fig. 2. SOD like activity(%) of AJNR and AJNS depending on concentration. Exceptionally AJNR was higher on 1000 ㎍/㎖ than AJNS. Results are expressed as mean±SD of triplicate experiments.
그림 Fig. 3-1. Cell viability of depending on concentration of AJNR and AJNS using MTT assay(Raw 264.7). The data illustrate the mean±SD of three separate experiments.
그림 Fig. 4. NO Inhibitory effects of extracts of AJNR and AJNS on Raw 264.7 cells. The cells were incubated with LPS for 24 hours in the group with and without the extract to determine the NO level. Nor : LPS not influencd group, Cont : LPS influencd group. AJNR and AJNS extracted with 70% ethanol. The data show the mean±SD of three separate experiments. ^*p<0.05 compared with control.
그림 Fig. 5. MMP-1 inhibition rate of AJNR and AJNS on CCD-986sk fibroblasts. The cells were treated with various concentrations of AJNR and AJNS for 48 hours. The contents of MMP-1 in culture media was determined by the MMP-1 ELISA kit as detailed under the materials and methods. Nor : normal (not UVB), Cont : control (UVB, without the extract). The data showed the mean ± SD of three separate experiments. ^*p<0.05 compared with control.
그림 Fig. 6. Procollagen synthesis of AJNR and AJNS on CCD-986sk fibroblasts. The cells were incubated for 24 hours in DMEM medium containing 10% FBS, and they were treated with various concentrations of AJNR and AJNS for 48 hours. The supernatant was collected from each well and typeⅠprocollagen was determined by ELISA kit. Nor : normal (not UVB), Cont :control (UVB, without the extract). The data showed mean ± SEM of three different assays. ^*p <0.05 compared with control.

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