[논문]바이러스성출혈성패혈증 바이러스 감염에 대한 3-Methyl Catechol의 항바이러스성 활성

조세영; 민나래; 김영오; 김두운

doi:10.5657/kfas.2021.0644

바이러스성출혈성패혈증 바이러스 감염에 대한 3-Methyl Catechol의 항바이러스성 활성
Anti-Viral Hemorrhagic Septicemia Virus (VHSV) Activity of 3-Methyl Catechol 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.54 no.5, 2021년, pp.644 - 651

조세영 (전남대학교 식중독바이러스연구센터) , 민나래 (전남대학교 식품공학과) , 김영오 (전남대학교 식품공학과) , 김두운 (전남대학교 식중독바이러스연구센터)

Abstract ▼ AI-Helper

Viral hemorrhagic septicemia virus (VHSV) is a fish pathogen responsible for causing enormous economic loss to the aquaculture industry not only in Korea but worldwide. Thus, it is necessary to identify natural compounds that can be used to control the spread of VHSV. In this study, the anti-VHSV activities of five catechol derivatives, i.e., catechol, pyrogallol, 3-methyl catechol, veratrole, and 3-methyl veratrole-extracted from green tea-were assessed. The antiviral activities of these derivatives were found to be dependent on their structure, i.e., the hydroxyl or methoxyl group and their substituent groups-on the benzene ring. Catechol, pyrogallol, and 3-methyl catechol exhibited relatively high 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities than veratrole, and 3-methyl veratrole. Moreover, 3-methyl catechol harboring a methyl substituent group increased the viability of the virus-infected cells and resulted in a 2.86 log reduction in the gene copies of VHSV N (per mL) in real-time PCR analysis. In conclusion, the catechol derivatives harboring hydroxyl groups in their benzene ring exhibited higher antioxidant activities than those harboring the methoxyl groups. However, catechol derivatives with a methyl group at the 3'-position of the benzene ring exhibited higher antiviral activity than those harboring a hydroxyl group. To our knowledge, this is the first study to evaluate the relationship between the structure and the anti-VHSV activity of catechol derivatives.

주제어

표/그림 (6)

그림 Fig. 1. Basic structure of flavonoids in polyphenols (with B-ring, A), catechol (B), pyrogallol (C), 3-methyl catechol (D), veratrole (E) and 3-methyl veratrole (F).
그림 Fig. 2. Antioxidant activity measurement using DPPH radicalscavenging assay. 0.2 mM of DPPH and 10 μM of each phenolic compound dissolved in methanol were mixed. The mixture reacted for 30 min and the absorbance at 517 nm using a spectrophotometer. Alpha-tocopherol (α-Toc) used as a standard reference. Different letters in a column are significantly different (P<0.05).
그림 Fig. 3. Evaluation of cell viability against VHSV infection in FHM cells treated with 100 μM of 5 catechol derivatives using CCK-8 assay. 0.1% of DMSO used as a control. Bars with different lowercase letters (a, b, and c) refer to significant differences (one-way ANOVA, Tukey's multiple comparison test, P<0.05) among the treatments, where the same letter indicates no significant difference.
그림 Fig. 4. DCFH-DA assay for detection of intracellular ROS produced in response to VHSV infection in FHM cell line. Cells were pre-treated with 100 μM of 3-methyl catechol (3-MC) and then infected with VHSV for 2 h. White bars refer to DMSO-treated cells as a control. Values are expressed as fluorescence intensity compared with a Mock-infected cell with non-treated 3-methyl catechol. Asterisk (*) indicates a significant value as determined by Dunnett's multiple comparison test (P<0.01).
그림 Fig. 5. Absolute viral (VHSV N gene) copy numbers were determined by RT-qPCR. The 3-methyl catechol (3-MC) was pretreated for 12 h prior to viral infection. The FHM cells were infected with VHSV at on MOI of 0.2 for 42 h. 0.1% of DMSO used as a control. Bars with different lower-case letters (a, b, and c) refer to significant differences (one-way ANOVA, Tukey's multiple comparison test, P<0.05) among the treatments, where the same letter indicates no significant difference.
표 Table 1. Cytotoxicity and protective effect of 3-methyl catechol against VHSV

AI 본문요약
AI-Helper

문제 정의

따라서, 본 연구는 VHSV 감염에 따른 양식어류의 대량폐사 저감화를 위하여 항산화 활성이 우수한 catechol 유도체들의 VHSV 증식 억제 여부를 평가하였다. 특히, 3-methyl catechol은 우수한 ROS 제거능과 함께 VHSV 감염에 따른 세포사멸을 억제함으로써 항바이러스 효과를 규명하였다.

제안 방법

따라서, 본 연구는 VHSV 감염에 따른 양식어류의 대량폐사 저감화를 위하여 항산화 활성이 우수한 catechol 유도체들의 VHSV 증식 억제 여부를 평가하였다. 특히, 3-methyl catechol은 우수한 ROS 제거능과 함께 VHSV 감염에 따른 세포사멸을 억제함으로써 항바이러스 효과를 규명하였다. 이는 양식어류에서 VHSV 감염을 제어하기 위한 사료첨가제로 활용될 것으로 사료된다.

데이터처리

모든 분석결과는 GraphPad Prism 프로그램(Ver. 5.01; Cary, NC, USA)을 이용하여 평균과 표준편차로 나타내었다. 시료 간의 유의성은 one-way ANOVA와 사후검정(Tukey 및 Dunnet multiple range test)을 실시하였으며, P<0.
모든 분석결과는 GraphPad Prism 프로그램(Ver. 5.
시료 간의 유의성은 one-way ANOVA와 사후검정(Tukey 및 Dunnet multiple range test)을 실시하였으며, P<0.05 수준에서 유의성을 평가하였다.

이론/모형

1). DPPH radical scavenging assay를 실시하여 catechol 유도체의 구조적 특성에 따른 항산화 활성을 검토하였다 (Bortolomeazzi et al., 2007).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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