[논문]내소황련탕(內疎黃連湯)의 항노화 및 항산화 효능 연구

오솔라; 남상우; 송영채; 김희택; 김용민; 이천일

doi:10.6114/jkood.2021.34.4.012

내소황련탕(內疎黃連湯)의 항노화 및 항산화 효능 연구
Anti-aging and Anti-oxidative Effect of Naesohwangryun-tang(NSHRT) 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.34 no.4, 2021년, pp.12 - 23

오솔라 (세명대학교 화장품과학과) , 남상우 (세명대학교 화장품뷰티생명공학부) , 송영채 (세명대학교 한의과대학 한방안이비인후피부과) , 김희택 (세명대학교 한의과대학 한방안이비인후피부과) , 김용민 (세명대학교 화장품뷰티생명공학부) , 이천일 (세명대학교 화장품뷰티생명공학부)

Abstract ▼ AI-Helper

Objectives : This study is to investigate the anti-aging and antioxidant effects of Naesohwangryeon-tang(NSHRT) extract in Hs68 fibroblasts. Methods : The fibroblast cell line Hs68 cells were used and MTT analysis was performed to measure the cell viability of Naesohwangryeon-tang, and then MMP-1 was induced with the cytokine TNF-𝛼 to confirm the anti-aging effect through real-time PCR and ELISA. In addition, ROS was induced with the TNF-𝛼 to confirm the anti-oxidant effect through DCF-DA. The signaling pathway according to the antioxidant effect was performed by western blot. Results : Naesohwangryeon-tang did not show cytotoxicity at all concentrations(100-800㎍/㎖) treated on Hs68 cells, and the inhibitory effect of MMP-1 was also confirmed. In addition, it was confirmed that the intracellular ROS increased by TNF-α was decreased by Naesohwangryeon-tang, and it was confirmed that the expression of NQO1 was increased in a concentration-dependent manner of Naesohwangryeon-tang. Conclusions : These results suggest that Naesohwangryeon-tang can be used as an anti-aging material by demonstrating the anti-aging and antioxidant effects of Naesohwangryeon-tang.

주제어

표/그림 (8)

표 Table 1. Contents of NSHRT
표 Table 2. Gene Name and Assay ID Number in Realtime PCR Analysis
그림 Fig. 1. Effect of NSHRT on Cell Viability in Hs68 Cells Cells were treated Naesohwangryun-tang(100-800㎍/ ㎖). Cells were incubated with 100, 200, 400 and 800㎍ /㎖ of NSHRT for 24 hours. Cell viability was measured using MTT assay.
그림 Fig. 2. The Effects of NSHRT on the mRNA Expression and Secretion of MMP-1 in TNF-α-Induced Hs68 Cells (A) NSHRT inhibits TNF-α-induced MMP-1 mRNA expression. (B) NSHRT inhibits TNF-α-induced MMP-1 secretion. Hs68 cells were pre-treated with NSHRT(100-800㎍/㎖) for 45 min, and then stimulated with TNF-α(20ng/㎖) for 24 hours. Forskolin was positive control. *p<0.05 compared with the TNF-α.
그림 Fig. 3. Effect of NSHRT on MAPK Signaling Pathway Expression in Hs68 Cells. (A) protein expression level, (B-D) ERK, JNK and p38 protein level was validated by image J. Hs68 cells were pre-treated with NSHRT(100-800㎍/㎖) for 45 min, and then stimulated with TNF-α(20ng/㎖) for 15 min. Forskolin was positive control. *p < 0.05 indicate a significant different compared with the TNF-α group of indicated proteins.
그림 Fig. 4. Effect of NSHRT on AP-1 Expression in Hs68 Cells. (A) protein expression level, (B, C) p-c-fos, p-c-jun protein level was validated by image J. Hs68 cells were pre-treated with NSHRT(100-800㎍/㎖) for 45 min, and then stimulated with TNF-α(20ng/㎖) for 15 min. Forskolin was positive control. *p < 0.05 indicate a significant different compared with the TNF-α group of indicated proteins.
그림 Fig. 5. Effect of NSHRT on Keap1 and NQO1 Expression in Hs68 Cells. (A) protein expression level, (B, C) Keap1, NQO1 protein level was validated by image J. Hs68 cells were treated for 24 hours with NSHRT(100-800㎍/㎖). Control: untreated group. *p < 0.05 indicate a significant different compared with the control group of indicated proteins.
그림 Fig. 6. The Effects of NSHRT on Intracellular ROS Production in Hs68 Cells. Hs68 cells were pre-treated with NSHRT(100-800㎍/㎖) for 45 min, and then stimulated with TNF-α(20 ng/㎖) for 24 hours.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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