[논문]스마트팜과 노지에서 재배한 병풀의 생약학적 비교

박진홍; 이다희; 조성민; 최정환; 김남준; 김민수; 박영민; 이기만

doi:10.22889/kjp.2022.53.4.192

스마트팜과 노지에서 재배한 병풀의 생약학적 비교
Comparison of Bioactivities from Centella asiatica Cultivated in Smart Farm and Field 원문보기

생약학회지, v.53 no.4, 2022년, pp.192 - 201

박진홍 (록야 주식회사) , 이다희 (록야 주식회사) , 조성민 (록야 주식회사) , 최정환 (록야 주식회사) , 김남준 (록야 주식회사) , 김민수 (록야 주식회사) , 박영민 (록야 주식회사) , 이기만 (록야 주식회사)

Abstract ▼ AI-Helper

This study aimed to compare bioactivities of Centella asiatica (CA) cultivated in smart farms and fields. Component analysis, cell viability, anti-inflammatory activity, neuroprotection activity, and antioxidant activity were examined with 70% ethanol extracts of CA cultivated in smart farm (SEE) and field (FEE), respectively. Asiaticoside was analyzed by high performance liquid chromatography (HPLC) and as a result, SEE had more asiaticoside content than FEE. After treatment of RAW 264.7 cells with SEE and FEE, there was no cytotoxicity within the treated concentrations. SEE and FEE showed nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 inhibitory activities in a dose-dependent manner in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Moreover, SEE inhibited more NO, TNF-α, and IL-6 production levels than FEE. SEE and FEE reversed the H2O2-induced SH-SY5Y cell death. Especially, SEE was more effective in changing the H2O2-induced SH-SY5Y cell death than FEE. The antioxidant activity was confirmed by various methods such as total phenol content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and superoxide dismutase (SOD). As a result, SEE showed the most potent antioxidant activities about TPC, DPPH, and SOD methods. This study suggested that SEE has higher bioactivities such as effect of anti-inflammation, neuroprotection, and antioxidation than FEE.

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표/그림 (10)

그림 Fig. 1. Growth condition and morphological characteristics of Centella asiatica.
표 Table I. HPLC condition for determination of asiaticoside
표 Table II. Comparison of asiaticoside content by different cultivation methods in Centella asiatica
그림 Fig. 2. HPLC chromatograms of asiaticoside by different cultivation methods in Centella asiatica. Quantitative analysis of asiaticoside was performed using a Agilent 1260 HPLC system consisting of diode array detector(210 nm), quaternary pump, autosampler and Luna C₁₈ column(250 mm×4.6 mm, 5 μL). The gradient elution was performed by using water(solvent A) and acetonitrile (solvent B). (A) HPLC chromatogram of asiaticoside standard, (B) HPLC chromatogram of asiaticoside in SEE, (C) HPLC chromatogram of asiaticoside in FEE. SEE; 70% ethanol extract of Centella asiatica cultivated in a smart farm, FEE; 70% ethanol extract of Centella asiatica cultivated in a field.
그림 Fig. 3. Inhibitory effects of SEE and FEE on NO production in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were pretreated with SEE and FEE for an hour then stimulated 1 μg/mL of LPS for 24 hours. Results were the means±SD(n=3). p-values were calculated using the Student's t-test. *p<0.05, **p<0.01, ***p<0.001 compared to the LPS treated group. (A) SEE, (B) FEE.
그림 Fig. 4. Inhibitory effects of SEE and FEE on TNF-α production in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were pretreated with SEE and FEE for an hour then stimulated 1 μg/mL of LPS for 24 hours. TNF-α production was estimated by ELISA kit. Results were the means±SD(n=3). p-values were expressed by the Student's t-test. *p<0.05, **p<0.01 compared to the LPS treated group. (A) SEE, (B) FEE.
그림 Fig. 5. Inhibitory effects of SEE and FEE on IL-6 production in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were pretreated with SEE and FEE for an hour then stimulated 1 μg/mL of LPS for 24 hours. IL-6 production was estimated by ELISA kit. Results were the means±SD(n=3). p-values were expressed by the Student’s t-test. ***p<0.001 compared to the LPS treated group. (A) SEE, (B) FEE.
그림 Fig. 6. Effects of H₂O₂ on cell viability in SH-SY5Y cells. Cell viability was estimated by the MTT assay. Cells were treated with H₂O₂ for 24 hours. The results were shown as percentage of the untreated control. Data was the means±SD(n=3). p-values were determined using the Student's t-test. **p<0.01, ***p<0.001 compared to untreated control.
그림 Fig. 7. Effects of SEE and FEE in H₂O₂-treated SH-SY5Y cells. Cell viability was determined by the MTT assay. Cells were treated with H₂O₂ for 24 hours then treated with SEE and FEE. Data was expressed as percentage of the untreated control. The results were the means±SD(n=3). p-values were calculated by the Student's t-test. **p<0.01 compared with H₂O₂-treated group.
표 Table III. Antioxidant activities of SEE and FEE

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문제 정의

따라서 본 연구에서는 당사의 스마트팜과 노지에서 각각 재배된 병풀을 70% 에탄올로 추출한 후 지표성분인 asiaticoside 함량을 측정하고 항염증 및 항산화 등의 다양한 생리활성을 비교하였다. 이로써 병풀의 산업적 활용 가능성을 타진하고 스마트팜에서 재배된 병풀을 수입대체 방안으로 제시하고자 하였다.

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