[논문]녹두 추출물이 생쥐에 유발된 접촉피부염의 피부 증상과 조직병리학적 소견에 미치는 영향

장선경; 강윤형; 오승엽; 강윤태; 김소연; 류지효; 김형우

doi:10.6116/kjh.2022.37.1.11.

녹두 추출물이 생쥐에 유발된 접촉피부염의 피부 증상과 조직병리학적 소견에 미치는 영향
Effects of Green Gram Extract on Skin Lesion and Histopathological Changes in Mice with Contact Dermatitis 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.1, 2022년, pp.11 - 18

장선경 (부산대학교 한의학전문대학원) , 강윤형 (부산대학교 한의학전문대학원) , 오승엽 (부산대학교 한의학전문대학원) , 강윤태 (부산대학교 한의학전문대학원) , 김소연 (부산대학교 한의학전문대학원) , 류지효 (부산대학교 한의학전문대학원) , 김형우 (부산대학교 한의학전문대학원)

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Objectives : Green gram (mung bean) has a cold nature and has been known to detoxify against various side effects that belong to hot in nature. In particular, since it has the effect of detoxifying fever and detoxification to treat swelling, it was also used externally to treat febrile dermatological diseases such as erysipelas and rubella. This study was designed to determine whether green gram exhibits anti-inflammatory effects on contact dermatitis in mice. Methods : We investigated the effects of green gram extract (70% ethanol extract) on skin lesion, skin thickness and weights, melanin and erythema index and spleen body weight ratio in mice with contact dermatitis induced by repeated application of 1-Fluoro-2,4-dinitrobenzene. Results : Topical application of green gram extract ameliorates skin lesions of contact dermatitis such as scale and roughness induces by 1-Fluoro-2,4-dinitrobenzene. green gram extract also suppressed enlargement of skin thicknesses and weights significantly. In addition, green gram extract treatment also lowered erythema index significantly compared to those in the control group. In the histopathological observation, green gram extract prevented epidermal hyperplasia and hyperkeratosis in inflamed tissues. Finally, green gram extract did not affect changes in body weights and the spleen body weight ratio, unlike dexamethasone, which significantly prevented body weight gain and lowered the spleen body weight ratio. Conclusions : These results imply that green gram, which is known to have a detoxifying effect in Korean medicine, can be used in the treatment of contact dermatitis.

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그림 Fig. 1. Experimental schedule The experimental groups except normal group, were sensitized by painting DNFB on days 1, 2 and 3, then challenged on days 8, 10, 12 and 14. GGE and DEX were topically treated for 6 days. The normal and control groups were treated with vehicles. All animals were sacrificed on day 15. GGE means green gram 70% ethanol extract. DNFB means 1-Fluoro-2, 4-dinitrofluorobenzene. S means sacrifice.
그림 Fig. 2. Effects of GGE on skin lesion in CD mice. Skin lesions of CD mice were observed using digital camera on day 15. A, normal; B, control; C, green gram extract; D, dexamethasone.
그림 Fig. 3. Effects of GGE on skin thicknesses and weights in CD mice. Effects on skin thicknesses and weights by GGE were analyzed on day 15. NOR, non-treated normal mice; CTL, CD induced control mice; GGE, 10 mg/ml of green gram extract treated CD mice; DEX, 2.5 mg/ml of dexamethasone treated CD mice. Values are represented as mean ± SD. ^###P < 0.001 vs. NOR, *P < 0.05, **P < 0.01 and ***P < 0.001 vs. CTL (n=8).
그림 Fig. 4. Effects of GGE on erythema and melanin index in CD mice. Erythema and melanin index were measured using dermospectrophotometer on day 15. NOR, non-treated normal mice; CTL, CD induced control mice; GGE, 10 mg/ml of green gram extract treated CD mice; DEX, 2.5 mg/ml of dexamethasone treated CD mice. Values are represented as mean ± SD. ^###P < 0.001 vs. NOR, *P < 0.05 vs. CTL (n=8). A.U. means arbitrary unit.
그림 Fig. 5. Effects of GGE on histopathological changes in inflamed tissues. Skin tissues were stained with hematoxylin and eosin then, observed by photo-microscope (100x). A, NOR; B, CTL; C, GGE; D, DEX. CTL means control. GGE means green gram extract, DEX means dexamethasone. Yellow bars mean thicknesses of epithelia. Filled arrows mean area of hyperkeratosis.
그림 Fig. 6. Effects of GGE on changes in body weights and spleen/body weight ratio in CD mice. Body weights and Spleen/body weight ratio were measured using electronic scales. Changes in body weights were based on the average weights on day 15 and expressed as percentages of weight on day 1 (A). Spleen weights were also measured on day 15, at which time the spleen/body weight ratio was calculated (B). NOR, non-treated normal mice; CTL, CD induced control mice; GGE, 10 mg/ml of green gram extract treated CD mice; DEX, 2.5 mg/ml of dexamethasone treated CD mice. Values are represented as mean ± SD. ^##P < 0.01 and ^###P < 0.001 vs. NOR, ***P < 0.001 vs. CTL (n=8).

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녹두 추출물이 생쥐에 유발된 접촉피부염의 피부 증상과 조직병리학적 소견에 미치는 영향
Effects of Green Gram Extract on Skin Lesion and Histopathological Changes in Mice with Contact Dermatitis 원문보기

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녹두 추출물이 생쥐에 유발된 접촉피부염의 피부 증상과 조직병리학적 소견에 미치는 영향 Effects of Green Gram Extract on Skin Lesion and Histopathological Changes in Mice with Contact Dermatitis 원문보기

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녹두 추출물이 생쥐에 유발된 접촉피부염의 피부 증상과 조직병리학적 소견에 미치는 영향
Effects of Green Gram Extract on Skin Lesion and Histopathological Changes in Mice with Contact Dermatitis 원문보기

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