[논문]콩 포장에서 어리팥나방과 팥나방(나비목: 잎말이나방과)의 동시 발생과 시토크롬 c 산화효소 1 유전자 염기서열의 다형성

정진교; 김은영; 한태만

doi:10.5656/ksae.2022.11.0.062

콩 포장에서 어리팥나방과 팥나방(나비목: 잎말이나방과)의 동시 발생과 시토크롬 c 산화효소 1 유전자 염기서열의 다형성
Co-occurrence of Matsumuraeses falcana and M. phaseoli (Lepidoptera: Tortricidae) in Soybean Fields, and Polymorphism of Cytochrome c Oxidase Subunit 1 Gene Nucleotide 원문보기

한국응용곤충학회지 = Korean journal of applied entomology, v.61 no.4, 2022년, pp.641 - 649

정진교 (국립식량과학원 재배환경과) , 김은영 (국립식량과학원 재배환경과) , 한태만 (국립공원연구원)

초록
AI-Helper

미토콘드리아 시토크롬 c 산화효소 1 (COX1) 유전자 염기서열(658 bp)을 사용하여, 콩 포장에서 채집된 어리팥나방(Matsumuraeses falcana)과 팥나방(Matsumuraeses phaseoli)의 종을 실험실 집단의 종들과 비교하여 동정하였다. COX1 염기서열 분석에서, 어리팥나방 47개체로부터 10개의 하플로타입이 발견되었고, 종내 유전적 거리는 0.15~0.46%이었다. 이중 하프로타입 A형이 약 70%로 우점형이었다. 팥나방의 30개체로부터는 모두 동일한 하나의 서열만이 확인되었고, 어리팥나방과의 종간 유전적 거리는 4.11~4.61%이었다. 두 종의 COX1 염기서열을 번역한 아미노산 서열은 모두 동일하여 동의적 염기서열 변이(동의치환, 同義置換, synonymous substitution)를 확인할 수 있었다. 포장 조사에서 두 종의 유충이 콩의 잎과 꼬투리를 가해하였고, 한 포장에서 동시에 발생하였다. 전체 포장에서 어리팥나방의 평균 밀도는 팥나방보다 약 1.5배 높았다. 이 결과는 콩이 두 종의 동일 기주임을 명백하게 제시하였다. 별도로 이 속의 유충 기생파리로서 Elodia flavipalpis (파리목: 기생파리과)가 발견되었고, COX1 서열로 동정되었다.

Abstract ▼ AI-Helper

Leaf-rolling moths were collected from soybean fields and identified as Matsumuraeses falcana and Matsumuraeses phaseoli by comparison with laboratory-reared species based on the nucleotide sequence (658 bp) of the mitochondrial cytochrome c oxidase 1 subunit gene (COX1). Ten haplotypes with 0.15-0.46% genetic distance from each other in COX1 were found in 47 samples of M. falcana, in which haplotype A was dominant (approximately 70%). Only one type of COX1 was found in 30 samples of M. phaseoli, and its sequence showed 4.11-4.61% genetic distance from those of M. falcana. Amino acid sequences translated from COX1 were identical in all samples of both species, and they showed synonymous substitutions. Larvae of both species caused damage to soybean leaves and pods and co-occurred simultaneously in the field. The average density of M. falcana was 1.5 times higher than that of M. phaseoli. The results clearly indicate that soybean was the host plant for both species. In addition, Elodia flavipalpis (Diptera: Tachinidae) was found to be a larval parasitoid of Matsumuraeses sp. through identification of the COX1 gene.

주제어

표/그림 (6)

그림 Fig. 1. Photographs of soybean plants injured by Matsumuraeses spp. larvae in the field. A and B: leaf damage, Baengnyeong; C and D: pod damage, Yeoncheon.
표 Table 1. Soybean damage ratios and densities of Matsumuraeses spp. observed in several soybean fields in the years 2020 and 2021
그림 Fig. 2. Polymorphism in the nucleotides (658 bp) of cytochrome oxidase subunit 1 gene of Matsumuraeses spp., and those translated amino acids and DNA codons. The two sequences of M. falcana haplotype A and M. phaseoli (MP) were identical to the sequences (GenBank accession No. OP373141 and OP380047) of respective laboratory populations. Only sequence positions (P.) with substituted nucleotides are presented, compared to the corresponding positions of haplotype A of M. falcana. The sequences of ten haplotypes in M. falcana and one type in M. phaseoli identified in this study were registered with GenBank as follows; M. falcana A (accession No. OP373142, OP373144, OP373151, OP373154), B (OP373143, OP373145, OP373152), C (OP373155), D (OP373146), E (OP373147), F (OP373148), G (OP373149), H (OP373156), I (OP373153), J (OP373150), M. phaseoli (MP) (OP380048-OP380050). Three sequences of α (KF402839), β (KF401056), and γ (MT578848) were collected from GenBank. The first eight nucleotides of the 5´ region in γ accession were removed due to a presumed error and replaced with the hypothetically-same sequence with those of other Matsumuraeses spp. In addition, the final 16 nucleotides of the 3´ region in γ accession were removed for alignment.
표 Table 2. Similarities and genetic distances between nucleotide sequences (658 bp) of mitochondrial cytochrome c oxidase subunit 1 gene in Matsumuraeses spp.
그림 Fig. 3. Neighbor-joining relationships among partial cytochrome c oxidase subunit 1 sequences (658 bp) of several species in the tribe Grapholitini (Tortricidae, Olethreutinae), including Matsumuraeses spp. GenBank accessions were collected randomly from the database only for species recorded in Korea. Adoxophyes orana (Tortricinae, Archinini) was selected as an outgroup. Bootstrap estimation of 2,000 replicates were conducted using the Kimura 2-parameter model. The numbers on bars indicate bootstrap confidence values.
표 Table 3. Number of Matsumuraeses falcana and M. phaseoli identified simultaneously in the same soybean fields based on mitochondrial cytochrome c oxidase subunit 1 gene (COX1) sequences

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