[논문]네트워크 약리학적 분석에 의한 소세포폐암에 대한 청대의 항암기전 연구

김영훈; 정우진; 정광희; 김윤숙; 안원근

doi:10.15188/kjopp.2022.12.36.6.229

네트워크 약리학적 분석에 의한 소세포폐암에 대한 청대의 항암기전 연구
Identifying the Anti-Cancer Effect of Indigo Naturalis in Small Cell Lung Cancer Based on Network Pharmacological Analysis 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.36 no.6, 2022년, pp.229 - 234

김영훈 (부산대학교 한의학전문대학원) , 정우진 (부산대학교 한의학전문대학원) , 정광희 (부산대학교 한의학전문대학원) , 김윤숙 (부산대학교 장수웰빙연구소) , 안원근 (부산대학교 한의학전문대학원)

Abstract ▼ AI-Helper

Lung cancer is the leading cause of cancer-related deaths worldwide. Indigo Naturalis (IN) is a dark blue powder obtained by processing leaves or stems of indigo plants, its anticancer effects have been reported in several studies. However, the pharmacological mechanism of IN in small cell lung cancer (SCLC) is not elucidated. In this study, to investigate the anticancer efficacy of IN for SCLC, we presented potential active ingredients, SCLC-related targets, and pharmacological mechanisms of IN that are expected to have anticancer activity for SCLC using a network pharmacological analysis. The phytochemical compounds of IN have been collected through TCMSP, SymMap, or HPLC documents. The active ingredients of IN such as indirubin, indican, isatin, and tryptanthrin were selected through ADME parameters or literature investigations for each compound. Using the Compounds, Disease-Target associations Databases, 124 common targets of IN and SCLC were obtained. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway enrichment analysis was carried out. GO biological processes are associated with response to xenobiotic stimulus, positive regulation of protein phosphorylation, regulation of mitotic cell cycle, and regulation of apoptotic signaling pathway. KEGG disease pathways included Gastric cancer, Bladder cancer, SCLC, and Melanoma. The main anticancer targets of the IN for SCLC were analyzed in 14 targets, including BCL2, MYC, and TP53. In conclusion, the results of this study based on the network pharmacology of IN can provide important data for the effective prevention and treatment of SCLC.

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표/그림 (7)

표 Table 1. Active Compounds of Indigo Naturalis
표 Table 2. Targets Related to SCLC and Active Compounds of Indigo Naturalis
그림 Fig. 1. Enrichment Analysis of GO and KEGG Pathway for 124 Common Targets. (A) GO enrichment analysis. (B) KEGG enrichment analysis.
그림 Fig. 2. C-T-BP Network. Active compound; Azure triangle with 16 nodes, Target; Pink circle with 124 nodes, Biological process; light green rhombus with 20 nodes.
그림 Fig. 3. PPI Network of 124 Common Targets. PPI network of IN-SCLC common targets: each node size is proportional to the degree and arranged in a circular shape from the inside according to the size, Total network was constructed with 124 nodes and 478 edges (confidence cutoff = 0.9).
표 Table 3. Active Compounds in Indigo Naturalis by Core Targets Involving in SCLC Pathway
그림 Fig. 4. Potential Pharmacological Mechanism of Indigo Naturalis for SCLC.

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