[논문]자소엽 추출물의 항균 및 항산화 효과와 성분분석

정효진; 현송화; 송바름; 이상래; 이윤주; 박수남

doi:10.14478/ace.2018.1077

자소엽 추출물의 항균 및 항산화 효과와 성분분석
Antimicrobial and Antioxidant Activities of Perilla frutescens var. acuta Extract and Its Fraction and Their Component Analyses 원문보기

공업화학 = Applied chemistry for engineering, v.29 no.6, 2018년, pp.716 - 725

정효진 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) , 현송화 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) , 송바름 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) , 이상래 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) , 이윤주 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) , 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소)

초록
AI-Helper

본 연구에서는 자소엽의 50% 에탄올 추출물 및 에틸아세테이트 분획을 제조하여 항균 및 항산화 활성을 평가하고 성분 분석을 진행하였다. 피부 상재균에 대한 항균 활성을 측정한 결과, S. aureus 및 P. aeruginosa에 대해서 에틸아세테이트 분획의 최소저해농도(minimum inhibitory concentration, MIC) 값은 모두 $78{\mu}g/mL$로 나타나 항균 활성이 매우 우수함을 확인하였다. 2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거 활성($FSC_{50}$)과 $Fe^{3+}-EDTA/H_2O_2$계에서의 활성산소 소거 활성($OSC_{50}$) 측정 결과 50% 에탄올 추출물 보다 에틸아세테이트 분획에서 높은 활성을 보였고 각각 25.90, $1.40{\mu}g/mL$로 나타났다. HaCaT 세포에서 세포 내 ROS 억제 효능은 최고 농도인 $32{\mu}g/mL$에서 50% 에탄올 추출물과 에틸아세테이트 분획 각각 PC군 대비 28.6, 40.7%의 감소율을 보였고 UVB에 대한 세포보호효과는 에틸아세테이트 분획의 $2.0-16.0{\mu}g/mL$ 농도에서 농도 의존적으로 나타났다. 자소엽 추출물의 에틸아세테이트 분획을 이용한 성분 분석 결과 rosmarinic acid, luteolin, apigenin, caffeic acid 및 ethyl caffeate가 있음을 확인하였다. 이상의 결과들은 자소엽 추출물 및 분획이 항균 및 항산화 효과를 가짐을 보여주며 외부 환경으로부터 피부를 보호할 수 있는 천연 소재로써 화장품 분야에서 응용 가능성이 있음을 시사한다.

Abstract ▼ AI-Helper

In this study, antimicrobial and antioxidative activities of Perilla frutescens var. acuta were investigated with 50% ethanol and the ethyl acetate fraction and also the components were analyzed. The minimum inhibitory concentration (MIC) of the ethyl acetate fraction for both Staphylococcus aureus and Pseudomonas aeruginosa were $78{\mu}g/mL$, indicating high antimicrobial effects. The free radical scavenging activity ($FSC_{50}$) and the reactive oxygen species (ROS) scavenging activity ($OSC_{50}$) in $Fe^{3+}-EDTA/H_2O_2$ system values of the ethyl acetate fraction were $25.90{\mu}g/mL$ and $1.40{\mu}g/mL$, respectively. After the cell damage induced by $400mJ/cm^2$ UVB irradiation, the cytoprotective effect of the ethyl acetate fraction of P. frutescens var. acuta showed the concentration dependent manner ranging from 2.0 to $16.0{\mu}g/mL$. The intracellular ROS inhibitory activity in HaCaT cells decreased to 28.6% and 40.7% for the 50% ethanol extract and ethyl acetate fraction, respectively at the concentration of $32{\mu}g/mL$. Components of rosmarinic acid, luteolin, apigenin, caffeic acid and ethyl caffeate were identified in the ethyl acetate fraction. These results suggest that the extract and fraction of P. frutescens var. acuta may be applied to the field of cosmetics as a natural material that protects the skin from an external environment by having antimicrobial and antioxidative activities.

주제어

표/그림 (14)

$Figure 1. Fractionation scheme of P. frutescens var. acuta 50% EtOH extract and ethyl acetate fraction.$ 그림 Figure 1. Fractionation scheme of P. frutescens var. acuta 50% EtOH extract and ethyl acetate fraction.
표 Table 1. HPLC Condition for Separation of 50% EtOH Extract and EtOAc Fraction of P. frutescens var. acuta Extract
$Figure 2. Free radical scavenging activities of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta and (+)-α-tocopherol. Data are presented as mean ± S.D. *p < 0.05 compared with (+)-α- tocopherol.$ 그림 Figure 2. Free radical scavenging activities of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta and (+)-α-tocopherol. Data are presented as mean ± S.D. *p < 0.05 compared with (+)-α- tocopherol.
표 Table 2. Minimum Inhibitory Concentration (MIC, μg/mL) and Minimum Bactericidal Concentration (MBC, μg/mL) of 50% EtOH Extract and Ethyl Acetate Fraction from P. frutescens var. acuta and Reference
표 Table 3. DPPH Radical Scavenging Activity of 50% EtOH Extract and Ethyl Acetate Fraction from P. frutescens var. acuta and Reference
$Figure 3. ROS scavenging activities of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta and L-ascorbic acid in Fe3+-EDTA/H2O2 system by luminol-dependent chemiluminescence assay. Data are presented as mean ± S.D. *p < 0.05 and **p < 0.01 compared with L-ascorbic acid.$ 그림 Figure 3. ROS scavenging activities of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta and L-ascorbic acid in Fe³⁺-EDTA/H₂O₂ system by luminol-dependent chemiluminescence assay. Data are presented as mean ± S.D. *p < 0.05 and **p < 0.01 compared with L-ascorbic acid.
$Figure 4. Effects of 50% EtOH extract and EtoAc fraction from P. frutescens var. acuta on HaCaT cells viability. HaCaT cells were treated with different concentration of samples for 24 h, and cytotoxicity was then determined by the MTT assay. Data are presented as mean ± S.D.$ 그림 Figure 4. Effects of 50% EtOH extract and EtoAc fraction from P. frutescens var. acuta on HaCaT cells viability. HaCaT cells were treated with different concentration of samples for 24 h, and cytotoxicity was then determined by the MTT assay. Data are presented as mean ± S.D.
표 Table 4. Reactive Oxygen Species Scavenging Activity of 50% EtOH Extract and Ethyl Acetate Fraction from P. frutescens var. acuta and Reference in Fe³⁺-EDTA/H₂O₂ System by Luminol-Dependent Chemiluminescence Assay
$Figure 5. Effects of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta on UVB-induced ROS generation in HaCaT cells. HaCaT cells were treated with different concentration of 50% EtOH extract and EtOAC fraction for 24 h, after being stimulated with UVB. ROS generation was measured by the fluorescence intensity of H2DCF-DA. Data are presented as mean ± S.D. *p < 0.05 compared with UVB treated control in 50% EtOH extract dose-treated group, #p < 0.05 compared with UVB treated control in EtOAc fraction dose-treated groups.$ 그림 Figure 5. Effects of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta on UVB-induced ROS generation in HaCaT cells. HaCaT cells were treated with different concentration of 50% EtOH extract and EtOAC fraction for 24 h, after being stimulated with UVB. ROS generation was measured by the fluorescence intensity of H₂DCF-DA. Data are presented as mean ± S.D. ^*p < 0.05 compared with UVB treated control in 50% EtOH extract dose-treated group, ^#p < 0.05 compared with UVB treated control in EtOAc fraction dose-treated groups.
$Figure 6. 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta treatment protect HaCaT cells against UVB-mediated decreased cell viability. HaCaT cells were treated with different concentration of samples for 24 h after being exposed to oxidative stress. Data are presented as mean ± S.D. *p < 0.05 compared with untreated control in 50% EtOH extract dose-treated group, #p < 0.05 compared with untreated control in EtOAc fraction dose-treated groups.$ 그림 Figure 6. 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta treatment protect HaCaT cells against UVB-mediated decreased cell viability. HaCaT cells were treated with different concentration of samples for 24 h after being exposed to oxidative stress. Data are presented as mean ± S.D. ^*p < 0.05 compared with untreated control in 50% EtOH extract dose-treated group, ^#p < 0.05 compared with untreated control in EtOAc fraction dose-treated groups.
표 Table 5. Bioactive Compounds Contents of P. frutescens var. acuta 50% EtOH Extract and Ethyl Acetate Fraction
$Figure 7. TLC chromatogram of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta and references. Eluent system; hexane : ethyl acetate : acetic acid = 24 : 14 : 5 (v/v), ① rosmarinic acid ② luteolin ③ 50% ethanol extract ④ ethyl acetate fraction ⑤ caffeic acid ⑥ apigenin ⑦ ethyl caffeate.$ 그림 Figure 7. TLC chromatogram of 50% EtOH extract and EtOAc fraction from P. frutescens var. acuta and references. Eluent system; hexane : ethyl acetate : acetic acid = 24 : 14 : 5 (v/v), ① rosmarinic acid ② luteolin ③ 50% ethanol extract ④ ethyl acetate fraction ⑤ caffeic acid ⑥ apigenin ⑦ ethyl caffeate.
$Figure 8. (a) Chromatogram of standards, (b) 50% EtOH extract, (c) EtOAc fraction from P. frutescens var. acuta extract at 254-400 nm. Peak 1, caffeic acid; peak 2, rosmarinic acid; peak 3, ethyl caffeate; peak 4, luteolin; peak 5, apigenin.$ 그림 Figure 8. (a) Chromatogram of standards, (b) 50% EtOH extract, (c) EtOAc fraction from P. frutescens var. acuta extract at 254-400 nm. Peak 1, caffeic acid; peak 2, rosmarinic acid; peak 3, ethyl caffeate; peak 4, luteolin; peak 5, apigenin.
표 Table 6. LC/ESI-MS and UV Spectrum Characteristics of Ethyl Acetate Fraction from P. frutescens var. acuta Extract

질의응답

핵심어	질문	논문에서 추출한 답변
	피부의 항산화 방어망은 어떤 손상을 방지하기 위해 구축되었는가?	미생물 외에도 일상생활 중에 많이 노출되는 자외선은 피부에 산화적 스트레스를 유발하고 노화를 가속화시킨다. 자외선에 노출된 피부에서는 활성산소종(reactive oxygen species, ROS)이 생성되고 단백질의 산화, DNA 손상, 지질 과산화 반응을 개시시킨다. 더욱이 matrix metalloproteinases (MMPs)의 발현이 촉진되어 콜라겐, 엘라스틴 섬유의 절단 및 비정상적인 교차결합을 일으키게 된다[6-8]. 이러한 손상을 방지하기 위해 피부에는 효소적 항산화제나 비효소적 항산화제와 같은 항산화 방어망이 구축되어 있으나 피부의 항산화 방어 수준을 압도하는 과잉의 ROS는 체내 항산화 네트워크의 균형을 깨뜨려 결국 피부 노화 및 여러 질병을 유발하게 된다[9].
	천연 항산화제에 관한 연구가 활발한 이유는 무엇인가?	그러므로 자외선으로부터의 피부 세포 보호 및 광노화 예방을 위해서는 항산화제의 적절한 보충이 꼭 필요하다. 인위적으로 보충이 가능한 항산화제는 크게 합성 항산화제와 천연 항산화제로 나눌 수 있는데, butylated hydroxyanisole(BHA) 및 butylated hydroxytoluene (BHT)과 같은 합성 항산화제들은 가격이 저렴하고 항산화 효과가 뛰어나 널리 이용되고 있지만 암을 유발하는 등 안전성에 대한 우려가 있어 최근에는 비교적 부작용이 적은 천연물로부터 천연 항산화제를 찾아내려는 연구들이 활발히 이루어지고 있다[10-11]. 한편, 식물유래 천연 항산화제 중 페놀성 화합물, 특히 폴리페놀의 일종인 플라보노이드는 지질의 산화 및 ROS로 유발되는 산화적 스트레스를 억제하여 노화를 방지하며 암을 예방하는 것으로 알려져 많은 연구가 수행되고 있으며 식품, 의약품 및 화장품 등의 소재로도 다양하게 활용되고 있다[12-14].
	자소엽은 무엇인가?	자소엽(Perilla frutescens var. acuta)은 차조기 또는 주름소엽이라불리는 인도, 중국, 일본, 베트남 및 한국과 같은 아시아 국가에서 주로 재배되는 꿀풀과의 한해살이풀로서, 예로부터 한방에서는 천식, 기침, 가래, 인후염, 소화불량, 요통, 불면증, 당뇨병 등의 처방에 이용되어 왔다[15-17]. 전통약용 식물로서 광범위한 분야에서 효능을 나타내는 자소엽은 항산화, 항염증 및 항노화 등의 효과가 나타나는 것으로 보고되었다[18-21].

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
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