[논문]국내 시판 숙취해소제 4종의 혈중 알코올 및 아세탈데히드 농도 감소 효능 비교

한민지; 진유정; 정세영

doi:10.5352/jls.2022.32.2.79

국내 시판 숙취해소제 4종의 혈중 알코올 및 아세탈데히드 농도 감소 효능 비교
Efficacy Verification of Four Hangover Cure Products for Reducing Blood Alcohol and Acetaldehyde Concentrations in Sprague - Dawley Rats 원문보기

생명과학회지 = Journal of life science, v.32 no.2, 2022년, pp.79 - 85

한민지 (경희대학교 일반대학원 나노의약생명과학과) , 진유정 (경희대학교 일반대학원 나노의약생명과학과) , 정세영 (경희대학교 일반대학원 나노의약생명과학과)

초록
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최근 알코올성 간손상이나 간건강에 효과가 있는 천연물 원료를 포함하는 숙취해소제들이 시판되고 있다. 하지만 간손상이나 간건강에 대한 기능성은 숙취해소와는 다르기 때문에 숙취해소제들의 숙취해소 효능 검증이 필요하다. 본 연구는 국내의 4가지 숙취해소제의 효능을 알코올에 의한 숙취유도 흰쥐 모델에서 비교 조사하고자 하였다. 4가지 샘플들은 각각 C, M, R, S로 명명되었다. 동물실험에서 숙취 완화 효능은 혈중 알코올 및 아세탈데히드 농도로 평가되기 때문에 각 샘플들의 효과를 혈중 알코올과 아세탈데히드의 농도와 AUC (Area Under the Curve)로 평가하였다. 알코올 투여는 유의적으로 흰쥐의 혈중 알코올과 아세탈데히드 농도를 증가시켰다. 샘플 C, M, R, S순으로 에탄올의 Cmax와 AUC 감소율은 각각 5.9%, 3.1%, 8.4%, 11.7%와 8.9%, 2.2%, 12.1%, 19.6%였다. 아세탈데히드의 경우, 같은 샘플 순서로 Cmax는 14.2%, 15.2%, 28.2%, 35.0% 감소하였으며, AUC는 21.6%, 7.5%, 22.4%, 29.9%의 감소율을 보였다. 결론적으로, 샘플 S, R, C, M순으로 4개의 샘플 모두 EtOH군에 대비하여 숙취가 해소되는 경향을 보였으나, 샘플 S만이 에탄올과 아세탈데히드의 혈중 농도와 AUC 모두에서 통계적으로 유의적인 감소율을 보였다. 따라서 이러한 실험결과는 시중에 판매되는 숙취해소제의 객관적인 효능 검증이 부족하다는 것을 시사한다.

Abstract ▼ AI-Helper

Recently, many hangover cure products containing natural ingredients have been made available in the market that are effective for alcohol-related liver damage or for improved liver function. However, the cure for of liver damage or medication for improved liver function are different from hangover cure. Therefore, the efficacy hangover cure products needs to be verified. In this study, we investigated and compared the ameliorating effect of four commercially available hangover cure products on acute ethanol-induced hangover in Sprague - Dawley rats. The four samples were labeled as C, M, R, and S. The efficacy of the samples was evaluated based on the serum concentration and area under the curve (AUC) of blood ethanol and acetaldehyde concentrations to quantitatively assess the hangover cure effect. Ethanol administration to the rats significantly raised the serum alcohol and acetaldehyde levels. The Cmax reduction rates of ethanol for the samples C, M, R, and S were 5.9%, 3.1%, 8.4%, and 11.7%, and the AUC were 8.9%, 2.2%, 12.1%, and 19.6%, respectively, whereas the Cmax reduction rates of acetaldehyde were 14.2%, 15.2%, 28.2%, and 35.0%, and the AUC were 21.6%, 7.5%, 22.4%, and 29.9%, respectively. In conclusion, all samples showed a tendency to relieve hangover in the order of S, R, C, and M in terms of the ethanol concentration, but only sample S showed a statistically significant decrease in both Cmax and AUC for ethanol and acetaldehyde. These results suggest that an objective method for verifying the efficacy of hangover cure products is lacking.

주제어

표/그림 (6)

표 Table 1. Detail information of hangover cure products
표 Table 2. Experimental group design
그림 Fig. 1. Effect of four hangover cure products on ethanol level. (A) Etanol concentration. (B) Ethanol area under the curve. Normal (Saline + 0.5% CMC); EtOH (Ethanol + 0.5% CMC); Sample S (Ethanol + Sample S (312.5 mg/kg body weight)); Sample C (Ethanol + Sample C (312.5 mg/ kg body weight)); Sample R (Ethanol + Sample R (1,250 mg/kg body weight)); Sample M (Ethanol + Sample M (1,527.3 mg/kg body weight)). All data were expressed as the mean ± SD (n=12). ### p<0.001 versus Normal, *p< 0.05 and ***p<0.001 versus EtOH.
그림 Fig. 2. Effect of four hangover cure products on acetaldehyde level. (A) Acetaldehyde concentration (B) Acetaldehyde area under the curve. Normal (Saline + 0.5% CMC); EtOH (Ethanol + 0.5% CMC); Sample S (Ethanol + Sample S (312.5 mg/kg body weight)); Sample C (Ethanol + Sample C (312.5 mg/kg body weight)); Sample R (Ethanol + Sample R (1,250 mg/kg body weight)); Sample M (Ethanol + Sample M (1,527.3 mg/kg body weight)). All data were expressed as the mean ± SD (n=12). ^###p<0.001 versus Normal, **p<0.01 versus EtOH.
표 Table 3. Cmax and Tmax of etanol
표 Table 4. Cmax and Tmax of acetaldehyde

AI 본문요약
AI-Helper

문제 정의

In addition, there are no individually recognized materials for hanover cure or hanover relieving from KFDA. Therefore, in this study, we aimed to objectively confirm whether these hangover cure products are actually effective on lowering blood ethanol and acetaldehyde concentration in acute alcohol-induced hangover animal model.

제안 방법

Alcohol administration was conducted 30 minutes after administering each sample. Blood was collected from tail vein at zero hour, one hour, three hours, five hours and eight hours after ethanol administration. Blood collected by time was placed in tubes and centrifuged at 2,259× g for 15 minutes at 4℃ to obtain serum.
Four hangover cure products used in this study are commercially available, and the single intake amount of each product was 3 g, 100 ml, 12 g, and 3 g in the order of sample C, M, R, and S. In order to find out the hangover-relieving effect according to one serving amount of each product intake, the administration dose of the rat was converted from the one serving amount of human based on a body surface area coefficient of rats and humans.
However, there is no objective verification of its efficacy. In this study, we attempted to compare commercially available four hangover cure products by confirming the ability to reduce the blood ethanol concentration and the blood acetaldehyde concentration, which are the causative materials of hangover. As a result, we figured out all four samples showed a tendency to relieve hangover in the order of sample S > R > C > M.
This experiment was conducted to investigate the effects of commercially available four hangover cure products on blood ethanol concentration. Fig.

대상 데이터

In this experiment, 250 to 300 g of eight weeks old SD rats (Sprague-Dawley, male) were obtained from Raonbio Co., Ltd. (Yongin, Korea).
After adapting to the breeding environment for a week, the experiment was conducted. The experimental animals in this study were bred with the approval of the Institutional Animal Care and Use Committee of Kyung Hee University (KHUASP(SE)-20-023).

데이터처리

Since the normalization was satisfied by the normalization distribution test, it was judged to be statistically significant when p<0.05 after analysis by the Turkey test using One way ANOVA.

성능/효과

The difference in the efficacy of the three hangover cure products containing the Hovenia dulcis is also thought to be due to the different Hovenia dulcis extract content for each product and the additional effect of sub-ingredients. Although this experiment was conducted in an animal model rather than clinical trial, these experiment results suggested the need for objective efficacy verification of hangouver cure product.
Acetaldehyde is a toxic metabolite of alcohol and is known to cause headaches after drinking alcohol. As a result of this experiment, when each hangover cure product was taken thirty minutes before ethanol intake, all samples lowered the blood acetaldehyde Cmax but, as same as ethanol result, only sample S showed a significant decrease in both Cmax and AUC.
As a result of this experiment, when each hangover cure product was taken thirty minutes before ethanol intake, all samples lowered the blood ethanol Cmax and AUC, but only one sample out of the four samples showed a significant decrease in both Cmax and AUC.

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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