Gahee Ryu
(Department of Medical Biomaterials Engineering, College of Bioimedical Science, Kangwon National University)
,
Choong Je Ma
(Department of Medical Biomaterials Engineering, College of Bioimedical Science, Kangwon National University)
Lysimachia christinae Hance was commonly used in Oriental medicine for treating the hepatitis virus, cholecystitis and cholagogic efficiency. According to the previous study, it possesses high antioxidant and anti-inflammatory activity. Simultaneous determination analytical method of isolated eight ...
Lysimachia christinae Hance was commonly used in Oriental medicine for treating the hepatitis virus, cholecystitis and cholagogic efficiency. According to the previous study, it possesses high antioxidant and anti-inflammatory activity. Simultaneous determination analytical method of isolated eight compounds, cynaroside (1), 2-(3,4-dimethoxyphenyl) ethyl O-α-L-arabinopyranosyl-(1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→3)] β-D-glucopyranoside (2), stearylester ricinoleic acid (3), (E)-4-(3,4-dimethoxyphenyl) but-3-en-1-yl palmitate (4), 2-hydroxy-24-methoxy-4-tetracosenoic acid (5), 2-hydroxy-24-propoxy-4-tetracosenoic acid (6), β-sitosterol (7), and androst-16-ene-3,6-diol (8) were established by using HPLC-DAD. This HPLC analysis was detected on a Dionex C18 column (5 ㎛, 120 Å, 4.6 mm × 150 mm) at 25℃. The mobile phase consisted of 0.1% trifluoroacetic acid and acetonitrile at a flow rate of 1 mL/min. Validation of the method was assessed by linearity, precision and accuracy test. Calibration curve was good at r2 > 0.9998. Limits of detection (LOD) ranged from 0.19 to 8.18 g/ml and Limits of quantification (LOQ) ranged from 0.19 to 24.80 g/ml. The relative standard deviations (RSD) values of precision test, intra- and inter- day, were less than 0.99% and 1.0%. The accuracy test results ranged from 98.81% to 106.49% and RSD values were less than 0.95%. These results showed that the HPLC-DAD method was very reliable and accurate for the quantity analysis of eight compounds in L. christinae extract for quality control.
Lysimachia christinae Hance was commonly used in Oriental medicine for treating the hepatitis virus, cholecystitis and cholagogic efficiency. According to the previous study, it possesses high antioxidant and anti-inflammatory activity. Simultaneous determination analytical method of isolated eight compounds, cynaroside (1), 2-(3,4-dimethoxyphenyl) ethyl O-α-L-arabinopyranosyl-(1→2)-O-[6-deoxy-α-L-mannopyranosyl-(1→3)] β-D-glucopyranoside (2), stearylester ricinoleic acid (3), (E)-4-(3,4-dimethoxyphenyl) but-3-en-1-yl palmitate (4), 2-hydroxy-24-methoxy-4-tetracosenoic acid (5), 2-hydroxy-24-propoxy-4-tetracosenoic acid (6), β-sitosterol (7), and androst-16-ene-3,6-diol (8) were established by using HPLC-DAD. This HPLC analysis was detected on a Dionex C18 column (5 ㎛, 120 Å, 4.6 mm × 150 mm) at 25℃. The mobile phase consisted of 0.1% trifluoroacetic acid and acetonitrile at a flow rate of 1 mL/min. Validation of the method was assessed by linearity, precision and accuracy test. Calibration curve was good at r2 > 0.9998. Limits of detection (LOD) ranged from 0.19 to 8.18 g/ml and Limits of quantification (LOQ) ranged from 0.19 to 24.80 g/ml. The relative standard deviations (RSD) values of precision test, intra- and inter- day, were less than 0.99% and 1.0%. The accuracy test results ranged from 98.81% to 106.49% and RSD values were less than 0.95%. These results showed that the HPLC-DAD method was very reliable and accurate for the quantity analysis of eight compounds in L. christinae extract for quality control.
Hakeem, K. R.; Abdul, W. M.; Hussain, M. M.; Razvi, S. S. I. In Oral health and herbal Medicine: Traditional information about herbal medicine of oral activity. Springer Briefs in Public Health. Springer, Switzerland, 2018, pp 17-18.?
Ghahremanlo, A.; Boroumand, N.; Ghazvini, K.; Hashemy, S. I. Phytother. Res. 2019, 33, 288-293.?
Lu, A. P.; Jia, H. W.; Xiao, C.; Lu, Q. P. World J. Gastroenterol. 2004, 10, 1854.?
Tuzimski, T. J. Sep. Sci. 2008, 31, 3537-3542.?
Houghton, P. J.; Hylands, P. J.; Mensah, A. Y.; Hensel, A.; Deters, A. M. J. Ethnopharmacol. 2005, 100, 100-107.?
Verpoorte, R.; Choi, Y. H.; Kim, H. K. J. Ethnopharmacol. 2005, 100, 53-56.?
Wang, Y. J.; Sun, Q. S. Chin. J. Med. Chem. 2005, 15, 357.?
Yeh, T. H.; Krauland, L.; Singh, V.; Zou, B.; Devaraj, P.; Stolz, D. B.; Franks, J.; Monga, S. P. S. Sasatomi, E.; Behari, J. Hepatology 2010, 52, 1410-1419.?
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