[논문]정향(丁香) 추출물이 골관절염 흰쥐에 미치는 효과 및 기전 연구

이진아; 김민주; 서성욱; 신미래

doi:10.22246/jikm.2022.43.6.1089

정향(丁香) 추출물이 골관절염 흰쥐에 미치는 효과 및 기전 연구
Study of the Effect and Underlying Mechanism of Clove Extract on Monosodium Iodoacetate-Induced Osteoarthritis in Rats 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.43 no.6, 2022년, pp.1089 - 1104

이진아 (대구한의대학교 한의과대학 본초약리학교실) , 김민주 (대구한의대학교 한의과대학 본초약리학교실) , 서성욱 (대구대학교 대학원 재활과학과) , 신미래 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective: The aim of this study was to identify the efficacy and underlying mechanism of cloves as an osteoarthritis (OA) treatment in a monosodium iodoacetate (MIA)-induced rat OA model. Osteoarthritis (OA) is nowadays one of the most prevalent degenerative joint diseases. Methods: Sprague-Dawley rats treated with MIA (50 μL; 80 mg/mL) were used as in vivo OA models. Cloves (100 and 200 mg/kg b.w.) were administered orally once daily for 2 weeks from 7 days after MIA injection. Changes in hindpaw weight distribution (HWD) were measured as a joint discomfort index. Activation markers related to inflammatory responses and cartilage degeneration in the right knee joints were evaluated by serum analysis and western blotting. Results: HWD decreased in the MIA control group but showed a dose-dependent elevation after clove treatment. Clove treatment inhibited inflammatory factors by PI3K/Akt/NF-κB signaling pathways, while also activating antioxidant factors through Sirt1/AMPK signaling pathways. Clove treatment also suppressed matrix metalloproteinase (MMP) overexpression and significantly increased the levels of tissue inhibitors of metalloproteinases (TIMPs). Conclusions: Treatment with cloves effectively reversed MIA-induced effects. Therefore, clove treatment could have the potential to protect against or treat OA.

주제어

표/그림 (10)

표 Table 1. The Changes of Body Weight and Food Intake
그림 Fig. 1. The changes in hind paw weight distribution in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body
그림 Fig. 2. Levels of serum ROS, ONOO-, and TBARS in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight. All data are expressed means±SEM (n=8). Significance : ^###p<0.001 vs. Normal group and *p<0.05, **p<0.01, ***p<0.001 vs. Control group
그림 Fig. 3. Expressions of NADPH oxidases in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight. All data are expressed means±SEM (n=8). Significance : ^#p<0.05, ^###p<0.001 vs. Normal group and *p<0.05, *p<0.01 vs. Control group
그림 Fig. 4. Expressions of PI3K and AKT in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight. All data are expressed means±SEM (n=8). Significance : ^##p<0.01, ^###p<0.001 vs. Normal group and *p<0.05, **p<0.01 vs. Control group
그림 Fig. 5. Expressions of NF-kBp65 and p-IkBɑ in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight. All data are expressed means±SEM (n=8). Significance : ^##p<0.01, ^###p<0.001 vs. Normal group and *p<0.05, **p<0.01 vs. Control group
그림 Fig. 6. Expression of inflammatory proteins in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight. All data are expressed means±SEM (n=8). Significance : ^##p<0.01, ^###p<0.001 vs. Normal group and *p<0.05, **p<0.01 vs. Control group
그림 Fig. 7. Expressions of anti-inflammatory proteins in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight. All data are expressed means±SEM (n=8). Significance : ^#p<0.05, ^##p<0.01 vs. Normal group and *p<0.05, **p<0.01 vs. Control group
그림 Fig. 8. Expressions of MMPs and TIMPs in MIA-induced osteoarthritis rats. Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight. All data are expressed means±SEM (n=8). Significance : #p<0.05, ^##p<0.01, ^###p<0.001 vs. Normal group and *p<0.05, **p<0.01, ***p<0.001 vs. Control group
그림 Fig. 9. Histopathological analysis in MIA-induced osteoarthritis rats. The representative images. Safranin O staining was performed. (magnification, ×50, ×100). Normal : normal rats, Control : MIA-induced osteoarthritis rats, Indo : MIA-induced osteoarthritis rats treated with indomethacin 5 mg/kg body weight, SA100 : MIA-induced osteoarthritis rats treated with SA 100 mg/kg body weight, SA200 : MIA-induced osteoarthritis rats treated with SA 200 mg/kg body weight.

참고문헌 (26)

Glyn-Jones S, Palmer AJ, Agricola R, Price AJ,？Vincent TL, Weinans H, et al. Osteoarthritis.？Lancet 2015;386(9991):376-87.

상세보기
He L, Pan Y, Yu J, Wang B, Dai G, Ying X.？Decursin alleviates the aggravation of osteoarthritis？via inhibiting PI3K-Akt and NF-kB signal pathway.？Int Immunopharmacol 2021;97:107657.

상세보기
Frasca D, Blomberg BB, Paganelli R. Aging,？Obesity, and Inflammatory Age-Related Diseases. Front Immunol 2017;8:1745.
Wang T, He C. Pro-inflammatory cytokines:？The link between obesity and osteoarthritis.？Cytokine Growth Factor Rev 2018;44:38-50.

상세보기
Vallabhapurapu S, Karin M. Regulation and？function of NF-kappaB transcription factors in？the immune system. Annu Rev Immunol 2009;27？:693-733.

상세보기
Yan W, Fan W, Chen C, Wu Y, Fan Z, Chen J,？et al. IL-15 up-regulates the MMP-9 expression？levels and induces inflammatory infiltration of？macrophages in polymyositis through regulating？the NF-kB pathway. Gene 2016;591(1):137-47.

상세보기
Hyeong K, Won JH, Woo CH. Anti-oxidant？and Anti-inflammatory Effects of Chulbu-tang.？J Korean Acad Rehabil Med 2020;30(3):71-87.
Chniguir A, Zioud F, Marzaioli V, El-Benna J,？Bachoual R. Syzygium aromaticum aqueous？extract inhibits human neutrophils myeloperoxidase？and protects mice from LPS-induced lung？inflammation. Pharm Biol 2019;57(1):56-64.

상세보기
Nunez L, Aquino MD. Microbicide activity of？clove essential oil (Eugenia caryophyllata). Braz？J Microbiol 2012;43(4):1255-60.
Ahmad T, Shinkafi TS, Routray I, Mahmood A,？Ali S. Aqueous Extract of Dried Flower Buds？of Syzygium aromaticum Inhibits Inflammation？and Oxidative Stress. J Basic Clin Pharm 2012;？3(3):323-7.

상세보기
Shin MR, Lee JA, Kim MJ, Park HJ, Park BW,？Seo SB, et al. Protective Effects of Phellinus？linteus Mycelium on the Development of Osteoarthritis？after Monosodium Iodoacetate Injection. Evid？Based Complement Alternat Med 2020;2020:7240858.
Ali SF, LeBel CP, Bondy SC. Reactive oxygen？species formation as a biomarker of methylmercury？and trimethyltin neurotoxicity. Neurotoxicology？1992;13(3):637-48.

상세보기
Kooy NW, Royall JA, Ischiropoulos H, Beckman JS.？Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med 1994;16(2):149-56.

상세보기
Mihara M, Uchiyama M. Determination of malonaldehyde？precursor in tissues by thiobarbituric acid test.？Anal Biochem 1978;86(1):271-8.

상세보기
Johnson VL, Hunter DJ. The epidemiology of？osteoarthritis. Best Pract Res Clin Rheumatol？2014;28(1):5-15.
Fei J, Liang B, Jiang C, Ni H, Wang L. Luteolin？inhibits IL-1β-induced inflammation in rat？chondrocytes and attenuates osteoarthritis progression？in a rat model. Biomed Pharmacother 2019;109？:1586-92.
Philpott HT, O'Brien M, McDougall JJ. Attenuation？of early phase inflammation by cannabidiol prevents？pain and nerve damage in rat osteoarthritis.？Pain 2017;158(12):2442-51.

상세보기
Xie Y, Shi X, Sheng K, Han G, Li W, Zhao Q,？et al. PI3K/Akt signaling transduction pathway,？erythropoiesis and glycolysis in hypoxia (Review).？Mol Med Rep 2019;19(2):783-91.
Zhu W, Hu X, Xu J, Cheng Y, Shao Y, Peng？Y. Effect of PI3K/Akt Signaling Pathway on？the Process of Prostate Cancer Metastasis to？Bone. Cell Biochem Biophys 2015;72(1):171-7.

상세보기
Kalbhen DA, Blum U. Theoretisches Konzept？und experimentell Bestatigung fur ein neues？Arthrose-Modell am Versuchstier [Hypothesis and？experimental confirmation of a new pharmacological？model of osteoarthrosis (author's transl)].？Arzneimittelforschung 1977;27(3):527-31.
Pitcher T, Sousa-Valente J, Malcangio M. The？Monoiodoacetate Model of Osteoarthritis Pain？in the Mouse. J Vis Exp 2016;(111):53746.
Woo YJ, Joo YB, Jung YO, Ju JH, Cho ML, Oh HJ, et al. Grape seed proanthocyanidin extract？ameliorates monosodium iodoacetate-induced？osteoarthritis. Exp Mol Med 2011;43(10):561-70.

원문보기 상세보기
Fernandes E, Toste SA, Lima JL, Reis S. The？metabolism of sulindac enhances its scavenging？activity against reactive oxygen and nitrogen？species. Free Radic Biol Med 2003;35(9):1008-17.

상세보기
Maneesh M, Jayalekshmi H, Suma T, Chatterjee？S, Chakrabarti A, Singh TA. Evidence for？oxidative stress in osteoarthritis. Indian J Clin？Biochem 2005;20(1):129-30.
van Dalen SCM, Kruisbergen NNL, Walgreen？B, Helsen MMA, Sloetjes AW, Cremers NAJ,？et al. The role of NOX2-derived reactive oxygen？species in collagenase-induced osteoarthritis.？Osteoarthritis Cartilage 2018;26(12):1722-32.

상세보기
Mehana EE, Khafaga AF, El-Blehi SS. The？role of matrix metalloproteinases in osteoarthritis？pathogenesis: An updated review. Life Sci 2019;？234:116786.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증