[논문]목과(木瓜)추출물이 급성 간손상 흰쥐에 미치는 효과

이진아; 신미래; 이지혜; 노성수

doi:10.22246/jikm.2021.42.1.1

목과(木瓜)추출물이 급성 간손상 흰쥐에 미치는 효과
The Effect of Chaenomelis Fructus Extract on Acute Hepatic Injury in Rats 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.42 no.1, 2021년, pp.1 - 10

이진아 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 이지혜 (한국한의학연구원 한약자원연구센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

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Objective: This study was conducted to investigate the effect of Chaenomelis Fructus (CF) water extract on thioacetamide (TAA)-treated rats. Methods: Rats were divided into five groups: one normal group (n=8) and four with TAA-induced hepatic injury. These treatment groups were administered distilled water (n=8); silymarin 100 mg/kg (n=8); CF 100 mg/kg (n=8); and CF 200 mg/kg (n=8). In the TAA groups, the acute liver injury was induced via IP injection (200 mg/kg), and the silymarin and CF extract were then orally administered for three days. Subsequently, serum levels of GOT, GPT, and ammonia were confirmed as well as protein expressions using liver tissue. Results: In the liver injury-induced rats, CF administration reduced tissue damage and serum levels of GOT, GPT, and ammonia. In addition, CF increased the anti-oxidant proteins Nrf2, Keap1, HO-1, and catalase and significantly regulated matrix metalloproteinases (MMP-2 and MMP-9) and their tissue inhibitors (TIMP-1 and TIMP-2). Conclusions: In this animal model of liver injury induced by TAA, CF extract is determined to have a hepatoprotective effect by increasing anti-oxidant proteins that relieve damage and by regulating the expression of matrix metalloproteinases.

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표/그림 (6)

표 Table 1. Scavenging Activity of Chaenomelis Fructus on DPPH and ABTS Radical
그림 Fig. 1. Histopathological analysis. Liver tissues were stained with hematoxyling and Eosin (Original magnification×200). Normal group; Normal, TAA-induced with distilled water group; Control, TAA-induced with silymarin 100 mg/kg group; Sily, TAA-induced with Chaenomelis Fructus 100 mg/kg group; CFL, TAA-induced with Chaenomelis Fructus 200 mg/kg group; CFH
그림 Fig. 2. Body weight change and liver weight. Body weight change; (A), liver weight; (B). All date are expressed mean±standard deviation (n=8). Normal group; Normal, TAA-induced with distilled water group; Control, TAA-induced with silymarin 100 mg/kg group; Sily, TAA-induced with Chaenomelis Fructus 100 mg/kg group; CFL, TAA-induced with Chaenomelis Fructus 200 mg/kg group; CFH. Significance: ^###p<0.001 vs. Normal group, *p<0.05, **p<0.01 vs. Control group
그림 Fig. 3. Expression of antioxidant-related protein in liver tissue. Nuclear factor erythroid 2-related factor 2; Nrf2, kelch-like ECH-associated protein 1; Keap1, heme oxygenase-1; HO-1. All date are expressed mean±standard deviation (n=8). Normal group; Normal, TAA-induced with distilled water group; Control, TAA-induced with silymarin 100 mg/kg group; Sily, TAA-induced with Chaenomelis Fructus 100 mg/kg group; CFL, TAA-induced with Chaenomelis Fructus 200 mg/kg group; CFH. Significance: #p<0.05, ^##p<0.01, ^###p<0.001 vs. Normal group, *p<0.05, **p<0.01, ***p<0.001 vs. Control group.
표 Table 2. GOT, GPT, and Ammonia Level in Serum
그림 Fig. 4. Expression of MMPs and TIMPs protein in liver tissue. Matrix metalloproteinase-2; MMP-2, matrix metalloproteinase-9; MMP-9, tissue inhibitor matrix metalloproteinase-1; TIMP-1, tissue inhibitor matrix metalloproteinase-2; TIMP-2. All date are expressed mean±standard deviation (n=8). Normal group; Normal, TAA-induced with distilled water group; Control, TAA-induced with silymarin 100 mg/kg group; Sily, TAA-induced with Chaenomelis Fructus 100 mg/kg group; CFL, TAA-induced with Chaenomelis Fructus 200 mg/kg group; CFH. Significance: ^##p<0.01, ^###p<0.001 vs. Normal group, *p<0.05, **p<0.01, ***p<0.001 vs. Control group.

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목과(木瓜)추출물이 급성 간손상 흰쥐에 미치는 효과
The Effect of Chaenomelis Fructus Extract on Acute Hepatic Injury in Rats 원문보기

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목과(木瓜)추출물이 급성 간손상 흰쥐에 미치는 효과 The Effect of Chaenomelis Fructus Extract on Acute Hepatic Injury in Rats 원문보기

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목과(木瓜)추출물이 급성 간손상 흰쥐에 미치는 효과
The Effect of Chaenomelis Fructus Extract on Acute Hepatic Injury in Rats 원문보기

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