[논문]계혈등 에탄올 추출물의 RANKL 처리 RAW264.7 세포의 분화와 염증성 골 손실에 미치는 영향

이대중; 황종현; 박도휘; 강기성; 전찬용; 황귀서

doi:10.22246/jikm.2022.43.6.1134

계혈등 에탄올 추출물의 RANKL 처리 RAW264.7 세포의 분화와 염증성 골 손실에 미치는 영향
Effect of Spatholobus Suberectus Extract (SSE) on RANKL-treated RAW264.7 and LPS-induced Bone Loss 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.43 no.6, 2022년, pp.1134 - 1148

이대중 (가천대학교 한의과대학 예방의학교실) , 황종현 (가천대학교 한의과대학 예방의학교실) , 박도휘 (가천대학교 한의과대학 예방의학교실) , 강기성 (가천대학교 한의과대학 예방의학교실) , 전찬용 (가천대학교 한의과대학 내과학교실) , 황귀서 (가천대학교 한의과대학 예방의학교실)

Abstract ▼ AI-Helper

Purpose: We evaluated whether Spatholobus suberectus extract (SSE) can be used as a means of preventing and treating osteoporosis by measuring its effect on osteoclast differentiation, gene expression, and bone resorption. Methods: SSE was used to examine the effect on RAW 264.7 cells stimulated with RANKL to induce bone resorption. The inhibitory effect of TRAP formation and the expression of the bone resorption factors TRAP, cathepsin K, and MMP-9 during differentiation were measured. The effects on the differentiation-related factors NFATc and TRAIL and on the expression of OC-STAMP, DC-STAMP, ATP6v0d2, MITF, c-Fos, and inflammation-related factors were also evaluated. The effect on bone resorption was evaluated by culturing RANKL-treated osteoclasts on artificial bone fragments and observing the resulting resorption traces. The effect on bone damage in experimental animals was also measured. Results: SSE inhibited the differentiation of RANKL-stimulated osteoclasts into osteoclasts and suppressed the expression of cathepsin K, TRAP, MMP-9, NFATc1, TRAIL, MITF, OC-STAMP, DC-STAMP, ATP6v0d2, and c-Fos genes. Bone pore formation due to osteoclast action was also inhibited, and LPS-induced bone loss was suppressed in animal experiments. Conclusions: SSE could be useful for the prevention or treatment of osteoporosis by inhibiting osteoclast differentiation and bone resorption and suppressing bone loss induced in experimental animals. However, studies of larger populations are required.

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표/그림 (16)

표 Table 1. Primers
그림 Fig. 1. Effects of SSE on cell viability of osteoclast. 0 (Control) : vehicle 9.375 : 9.375 ug/ml of SSE 18.75 : 18.75 ug/ml of SSE 37.5 : 37.5 ug/ml of SSE 75 : 75 ug/ml of SSE 150 : 150 ug/ml of SSE 300 : 300 ug/ml of SSE ## : p<0.01 vs Control
그림 Fig. 2. Effects of SSE on osteoclast differentiationin RANKL in duced RAW 264.7 cell.
그림 Fig. 3. Effects of SSE on the formation of TRAP(+) MNCs in RANKL induced osteoclast. 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ## : p<0.01 vs Control # : p<0.05 vs Control
그림 Fig. 4. Effects of SSE on the mRNA expression of cathepsin K in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 5. Effects of SSE on the mRNA expression of MMP-9 in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 6. Effects of SSE on the mRNA expression of TRAP in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 7. Effects of SSE on the mRNA expression of NFATc1 in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 8. Effects of SSE on the mRNA expression ofTRAIL in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 9. Effects of SSE on the mRNA expression of OC-STAMP in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 10. Effects of SSE on the mRNA expression of DC-STAMP in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 11. Effects of SSE on the mRNA expression of ATP6v0d2 in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 12. Effects of SSE on the mRNA expression of MITF in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control # : p<0.05 vs Control
그림 Fig. 14. Effects of SSE on bone pit formation by RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 30 : 30 ug/ml of SSE+100 ng/ml of RANKL 60 : 60 ug/ml of SSE+100 ng/ml of RANKL
그림 Fig. 13. Effects of SSE on the mRNA expression of c-FOS in RANKL induced osteoclast. NC : vehicle 0 (Control) : 100 ng/ml of RANKL 15 : 15 ug/ml of SSE+100 ng/ml of RANKL 35 : 35 ug/ml of SSE+100 ng/ml of RANKL 75 : 75 ug/ml of SSE+100 ng/ml of RANKL ** : p<0.01 vs NC ## : p<0.01 vs Control
그림 Fig. 15. In Vivo effect of SSE on LPS-induced bone degradation. NC : normal control CON : LPS only SSE : LPS+SSE (500 mg/kg)

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동의어: Packet Collision Rate

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