[논문]Biocontrol Activity of Aspergillus terreus ANU-301 against Two Distinct Plant Diseases, Tomato Fusarium Wilt and Potato Soft Rot

Choi, Hyong Woo; Ahsan, S.M.

doi:10.5423/ppj.oa.12.2021.0187

Biocontrol Activity of Aspergillus terreus ANU-301 against Two Distinct Plant Diseases, Tomato Fusarium Wilt and Potato Soft Rot 원문보기

The plant pathology journal, v.38 no.1, 2022년, pp.33 - 45

Choi, Hyong Woo (Department of Plant Medicals, College of Life Sciences and Biotechnology, Andong National University) , Ahsan, S.M. (Department of Plant Medicals, College of Life Sciences and Biotechnology, Andong National University)

Abstract ▼ AI-Helper

To screen antagonistic fungi against plant pathogens, dual culture assay (DCA) and culture filtrate assay (CFA) were performed with unknown soil-born fungi. Among the different fungi isolated and screened from the soil, fungal isolate ANU-301 successfully inhibited growth of different plant pathogenic fungi, Colletotrichum acutatum, Alternaria alternata, and Fusarium oxysporum, in DCA and CFA. Morphological characteristics and rDNA internal transcribed spacer sequence analysis identified ANU-301 as Aspergillus terreus. Inoculation of tomato plants with Fusarium oxysporum f. sp. lycopersici (FOL) induced severe wilting symptom; however, co-inoculation with ANU-301 significantly enhanced resistance of tomato plants against FOL. In addition, culture filtrate (CF) of ANU-301 not only showed bacterial growth inhibition activity against Dickeya chrysanthemi (Dc), but also demonstrated protective effect in potato tuber against soft rot disease. Gas chromatography-tandem mass spectrometry analysis of CF of ANU-301 identified 2,4-bis(1-methyl-1-phenylethyl)-phenol (MPP) as the most abundant compound. MPP inhibited growth of Dc, but not of FOL, in a dose-dependent manner, and protected potato tuber from the soft rot disease induced by Dc. In conclusion, Aspergillus terreus ANU-301 could be used and further tested as a potential biological control agent.

주제어

표/그림 (7)

그림 Fig. 1. Antifungal activity of ANU-3001. (A) Representative picture of DCA and CFA. (B) Growth inhibition percentage based on DCA and CFA.
그림 Fig. 2. Morphological and molecular identification of ANU-301 as Aspergillus terreus. (A-D) Photograph of colonial and conidiophore morphology on potato dextrose agar (PDA) medium. (A, B) ANU-301 showed white-colored mycelial growth on PDA medium. (A) Lower side. (B) Upper side. (C, D) Microscopic observation of conidiophore. Scale bars = 40 μm. (E) Internal transcribed spacer (ITS) amplification; M, marker 1 kb; 1, band of ITS (578 bp). (F) Phylogenetic tree based on ITS sequence of Aspergillus terreus ANU-301 (bold), its closely related Aspergillus species, and distantly related Penicillium spinulosum as outgroup.
그림 Fig. 3. Biocontrol activity of ANU-301 against tomato Fusarium wilt disease. (A) Representative picture of disease symptom developed on tomato plants inoculated with F. oxysporum f. sp. lycopersici (FOL) with or without ANU-301. (B) Fresh weight of tomato plants 7 dpi with FOL in the absence or presence of ANU-301. (C) Microscopic observation of mycelia of FOL during the dual culture assay with ANU-301. To show mycelial growth of FOL during DCA against ANU-301, part of Fig. 1A was enlarged. Scale bars = 20 μm.
그림 Fig. 4. Biocontrol activity of ANU-301 against potato soft rot disease. (A) Antibacterial activity of ANU-301 against Dickeya chrysanthemi (Dc) by agar plug diffusion assay. Diameter of zone of inhibition (ZOI) is shown below. (B) Growth inhibition of Dc by ANU-301 culture filtrate (CF). Plus (+) and minus (-) symbols mean presence and absence of 10% CF of ANU-301, respectively. (C, D) Biocontrol activity of CF of ANU-301 against potato soft rot disease caused by Dc. Representative disease symptoms (C) and severity (D) of potato tuber 2 weeks after inoculation with Dc in the presence or absence of CF of ANU-301. N/S, no symptom.
표 Table 1. List of 20 most abundant chemical compounds identified from CF of ANU-301 by GC-MS/MS analysis
그림 Fig. 5. Gas chromatography-tandem mass spectrometry chromatogram of culture filtrate of ANU-301. Chemical structure of the most abundant chemical compound 2,4-bis(1-methyl-1-phenylethyl)-phenol was shown above the peak (see Table 1 for the list of 20 most abundant chemical compound identified).
그림 Fig. 6. Biocontrol activity of 2,4-bis(1-methyl-1-phenylethyl)-phenol (MPP) against Dickeya chrysanthemi (Dc). (A) Antibacterial activity of culture filtrate and MPP (400 ppm) against Dc by disc diffusion assay. Diameter of zone of inhibition (ZOI) is shown below. (B) Concentration-dependent growth inhibition of MPP on Dc growth in nutrient broth (NB) media. Cell growth was measured by spectrophotometer at OD₆₀₀. Calculated IC₅₀ was 505.9 ppm. (C, D) Biocontrol activity of MPP against potato soft rot disease caused by Dc. Representative disease symptoms (C) and severity (D) of potato tuber 2 weeks after inoculation with Dc in the presence or absence of MPP. N/S, no symptom.

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저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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